Rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga through tissue culture

Abstract

An efficient protocol has been established for rapid multiplication and in vitro production of leaf biomass in Kaempferia galanga L, a rare medicinal plant. Different plant growth regulators like Benzyladenine (BA), Indoleacetic acid (IAA), Indolebutyric acid (IBA), Napthaleneacetic acid (NAA) and adenine sulphates (Ads) have been tried for induction of multiple shoots using lateral bud of rhizome as explants. The highest rate of shoot multiplication (11.5 ± 0.6) shoot/explant as well as leaf biomass production (7.4 ± 0.3) gram/explant was observed on Murashige and Skoog medium supplemented with Benzyladenine (1 mg/l) and Indoleacetic acid (0.5 mg/l). Data of shoot multiplication and leaf biomass production were statistically analysed. Upon excission of leaves after 2 months of culture under sterile condition, the base of each plantlet was transferred to fresh media which could produce the same leaf biomass within another 2 months in a 50 ml culture tube containing 20 ml and 250 ml conical flasks *Corresponding author containing 30 ml Murashige and Skoog medium. The rate of multiplication and leaf biomass production remained unchanged in subsequent subcultures. The regenerated plantlets were acclimatized in greenhouse and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 95 percent. Genetic fidelity of the regenerants was confirmed using random amplified polymorphic DNA (RAPD) marker. The protocol could be commercially utilized for large scale production of true-to-type plantlets and as an alternative method of leaf biomass production in Kaempferia galanga.Kaempferia galanga L. (K. galanga) is a rare Indian medicinal herb of family Zingiberaceae. It is distributed in the tropics and subtropics of Asia and Africa. The plant is extensively used in preparation of ayurvedic drugs, in perfumery, cosmetics and as spice ingredients (Rahman et al. 2004). The plant is used for treatment of diarrhea,

Influence of N6-Benzyladenine and Sucrose on In Vitro Direct Regeneration and Microrhizome Induction of Kaempferia parviflora Wall. Ex Baker, An Important Ethnomedicinal Herb of Asia

“…Pre-sterilisation technique and sterilisation using mercury chloride significantly reduced contamination in this study ( Table 1 ). In support of our observation, mercuric chloride (HgCl 2 ) was also reported to be beneficial in reducing contamination in in vitro culture of other Kaempferia species ( Shirin et al 2000 ; Chirangini et al 2005 ; Parida et al 2010 ). Mahmoud and Al-Ani (2016) found that HgCl 2 mainly has bactericidal action and is a more effective sterilant with better decontamination percentages compared to sodium hypochlorite.…”

Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

“…Comparatively, a favorable effect of IAA over other auxins in the present study is in consonance with the report in A. calcarata [13] and Curcuma haritha [29]. The inefficiency of NAA and IBA along with BAP in the present system was similar to the recent observations in Kaempferia galanga [30].…”

In vitropropagation ofHedychium coronariumKoen. through axillary bud proliferation

“…Shoot multiplication occurred by axillary bud proliferation maintains genetic stability in tissue cultures than by adventi tious bud formation (Balachandran et al 1990; Downloaded by [Laurentian University] at 19:07 09 October 2014 Shenoy & Vasil 1992;Joshi & Dhawan 2007). Molecular marker-based analysis of miropropagated and conventional grown K. galanga was reported by Mohanty et al (2010) and Parida et al (2010). ISSR markers are universal, quick to use, and easy to apply; they have the reproducibility of SSR markers due to the longer length of their primers (Bornet & Branchard 2001).…”