Rapid Multiplication of Basil (Ocimum Basilicum); Factors Affecting Callus Formation and Plant Regeneration

Lee, Ki-Jong; Yi, Bu-Young

doi:10.17660/actahortic.2003.625.31

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…‚Crispum', also popular called "Foglia di Lattuga" or "Lettuce Leaf" is recommended for culinary, ornamental, medicinal use, but also as a repellent (companion plant) for insects in organic farming, especially in tomato crops (DeBaggio, 1994;Bomford, 2009;Teodorescu et al, 2017). Approaches to basil multiplication through modern biotechnological in vitro multiplication techniques have been applied by researchers worldwide to both red basil varieties (Mathew, 2011;Saha et al, 2012;Ekmekci and Aasim, 2014) as well as those of green basil (Sahoo et al, 1997;Phippen and Simon, 2000;Begum et al, 2002;Dode et al, 2003;Lee and Yi, 2003;Gopi and Ponmurugan, 2006;Daniel et al, 2010;Mathew, 2011;Livadariu, 2011). Through this research, we developed organogenesis processes by in vitro cultures, using basil varieties of Romanian and international origin in the collection of improvement of the Research & Development Station for Vegetables Growing Buzau (SCDL Buzău), to analyze the morphogenetic response of explants (stems, leaves and cotyledonary node) detached from the young seedlings.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Organogenic Response to the Different Types of Explants From Romanian Varieties of Red and Green Basil

Dănăilă-Guidea¹,

Stan²,

Burnichi³

et al. 2020

CTNS

View full text Add to dashboard Cite

Basil is a plant known since ancient times for its properties (carminative, antiseptic), being considered a sacred plant. With the passage of time and with the diversification of assortments, basil was used for decorative and aromatic purposes, being considered a spicy plant. Red basil is also used in the perfume industry due to the pleasant aroma it possesses. Multiplication procedures were developed by in vitro cultures, using varieties of Romanian origin: 4 from red basil (lines: L9, L10, L11, L12) and 9 from green basil, characterized by special aromas and leaf shapes. When evaluating the germination yield, the percentages varied between 45-80% for aseptically inoculated red basil seeds on culture medium. And in the case of green basil varieties, the yield varied between 25 -100% after sowing in universal organic substrate. For the seeds of the green basil varieties, the sowing was performed in universal organic substrate, and the germination yield varied between 25 -100%. The types of explants taken from the resulting seedlings were represented by stems (fragments of hypocotyl and epicotyl), leaves and cotyledonary node. The induction of organogenesis was evaluated differently for each type of explant under the effect of phytohormones such as cytokinins (TDZ and BAP) added in concentrations of 0.5-2 mg / L to the basal culture medium MS / 1962. After 3 subcultures of the explants on the culture medium recipes (45 days), the organogenic yields expressed in vitro were compared

show abstract