Rapid nanomolar detection of methamphetamine in biofluids via a reagentless electrochemical aptamer-based biosensor

Xie, Yu; Wu, Shenghong; Chen, Zhimin; Jiang, Jinzhi; Sun, Jian‐Jun

doi:10.1016/j.aca.2022.339742

Cited by 36 publications

(21 citation statements)

References 47 publications

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“…E-AB sensors fabricated with the TL aptamer are selective. As in many previous models, , blood serum was chosen as our complex matrix model. The tests were performed with blood serum at room temperature over a concentration range of 20–600 nM.…”

Section: Resultsmentioning

confidence: 99%

Development of an Electrochemical, Aptamer-Based Sensor for Dynamic Detection of Neuropeptide Y

Seibold,

Abeykoon,

Ross

et al. 2023

ACS Sens.

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Section: Resultsmentioning

confidence: 99%

Development of an Electrochemical, Aptamer-Based Sensor for Dynamic Detection of Neuropeptide Y

Seibold,

Abeykoon,

Ross

et al. 2023

ACS Sens.

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“…The remaining four aptamer candidates showed a lower binding affinity that was not significant, where the dissociation constants recorded were higher than 1 micromolar. Nevertheless, an aptamer can exert a higher binding affinity at the nanomolar to sub-nanomolar level against the target, according to previous reports [ 53 , 61 , 62 ]. Interestingly, negative enthalpy changes (ΔH) and entropy changes (ΔS) were recorded for both of the aptamer candidates ( Table 3 ), indicating that the Gibbs free energy may be positive or negative, depending on the temperature.…”

Section: Discussionmentioning

confidence: 81%

Computationally Designed Anti-LuxP DNA Aptamer Suppressed Flagellar Assembly- and Quorum Sensing-Related Gene Expression in Vibrio parahaemolyticus

Yusof

Razali

Padzil

et al. 2022

Biology

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(1) Background: Quorum sensing (QS) is the chemical communication between bacteria that sense chemical signals in the bacterial population to control phenotypic changes through the regulation of gene expression. The inhibition of QS has various potential applications, particularly in the prevention of bacterial infection. QS can be inhibited by targeting the LuxP, a periplasmic receptor protein that is involved in the sensing of the QS signaling molecule known as the autoinducer 2 (AI-2). The sensing of AI-2 by LuxP transduces the chemical information through the inner membrane sensor kinase LuxQ protein and activates the QS cascade. (2) Methods: An in silico approach was applied to design DNA aptamers against LuxP in this study. A method combining molecular docking and molecular dynamics simulations was used to select the oligonucleotides that bind to LuxP, which were then further characterized using isothermal titration calorimetry. Subsequently, the bioactivity of the selected aptamer was examined through comparative transcriptome analysis. (3) Results: Two aptamer candidates were identified from the ITC, which have the lowest dissociation constants (Kd) of 0.2 and 0.5 micromolar. The aptamer with the lowest Kd demonstrated QS suppression and down-regulated the flagellar-assembly-related gene expression. (4) Conclusions: This study developed an in silico approach to design an aptamer that possesses anti-QS properties.

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“…Of note, although overnight incubation in MCH is commonly employed in the sequential deposition fabrication of EAB sensors, 4,6,13,18 some authors have used shorter periods. 17,19 We find, however, that longer incubation in MCH leads to improved performance for sensors fabricated using the sequential deposition method (Figure S1). Given this, we used overnight MCH incubation as the sequential deposition "standard" against which we performed our comparisons.…”

Section: ■ Results and Discussionmentioning

confidence: 90%

“…Historically, this has been performed by sequentially soaking the gold surface in a solution of a thiol-modified aptamer followed by a second incubation in dilute mercaptohexanol (MCH), which serves to “backfill” the space between aptamers to form a continuous monolayer (Figure ). Since it was first reported for the fabrication of DNA-modified SAMS, − this “sequential” method has dominated prior reports of EAB sensor fabrication, which typically employed MCH deposition times ranging from a few hours to overnight. ,,,− In contrast to this sequential method, however, there have also been reports in which other, similarly DNA-decorated gold surfaces (e.g., refs − ) and, more recently, even EAB sensors , have been fabricated via a “codeposition” method that employs single-step incubation in a mixture of MCH and thiol-modified aptamer (Figure ). To date, however, we have not seen any comparison of the performance of EAB sensors fabricated using these two distinct approaches.…”

Section: Introductionmentioning

confidence: 99%

Codeposition Enhances the Performance of Electrochemical Aptamer-Based Sensors

Wu,

Shi,

Kippin

et al. 2024

Langmuir

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Electrochemical aptamer-based (EAB) sensors, a minimally invasive means of performing high-frequency, real-time measurement of drugs and biomarkers in situ in the body, have traditionally been fabricated by depositing their target-recognizing aptamer onto an interrogating gold electrode using a "sequential" two-step method involving deposition of the thiol-modified oligonucleotide (typically for 1 h) followed by incubation in mercaptohexanol solution (typically overnight) to complete the formation of a stable, self-assembled monolayer. Here we use EAB sensors targeting vancomycin, tryptophan, and phenylalanine to show that "codeposition", a less commonly employed EAB fabrication method in which the thiol-modified aptamer and the mercaptohexanol diluent are deposited on the electrode simultaneously and for as little as 1 h, improves the signal gain (relative change in signal upon the addition of high concentrations of the target) of the vancomycin and tryptophan sensors without significantly reducing their stability. In contrast, the gain of the phenylalanine sensor is effectively identical irrespective of the fabrication approach employed. This sensor, however, appears to employ binding-induced displacement of the redox reporter rather than binding-induced folding as its signal transduction mechanism, suggesting in turn a mechanism for the improvement observed for the other two sensors. Codeposition thus not only provides a more convenient means of fabricating EAB sensors but also can improve their performance.

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Rapid nanomolar detection of methamphetamine in biofluids via a reagentless electrochemical aptamer-based biosensor

Cited by 36 publications

References 47 publications

Development of an Electrochemical, Aptamer-Based Sensor for Dynamic Detection of Neuropeptide Y

Development of an Electrochemical, Aptamer-Based Sensor for Dynamic Detection of Neuropeptide Y

Computationally Designed Anti-LuxP DNA Aptamer Suppressed Flagellar Assembly- and Quorum Sensing-Related Gene Expression in Vibrio parahaemolyticus

Codeposition Enhances the Performance of Electrochemical Aptamer-Based Sensors

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