Recent studies have highlighted the high sensitivity of PET imaging with 68 Ga-labeled octreotide derivatives for the detection and staging of neuroendocrine tumors. A somatostatin receptor ligand that is easily radiolabeled with 18 F-fluoride could improve the availability of PET imaging of neuroendocrine tumors. We report an alkyltrifluoroborateoctreotate conjugate that is radiolabeled in a 1-step 18 F exchange reaction in high yield and with high specific activity. Methods: We conjugated a new alkyltrifluoroborate to octreotate to obtain AMBF 3 -TATE, which was stored in 50-nmol aliquots for radiolabeling. 18 F labeling was performed by 18 F-19 F isotope exchange with 18 F-fluoride, and the tracer was purified by C18 cartridge separation. The radiochemical yield was 20%-25%. PET imaging and biodistribution were performed on mice bearing AR42J tumor xenografts. Results: AMBF 3 -TATE bound the somatostatin receptor subtype 2 with high affinity (inhibition constant, 0.13 ± 0.03 nM). Starting with 29.6-37 GBq (0.8-1 Ci) of 18 F-fluoride, more than 7.4 GBq (.200 mCi) of 18 F-AMBF 3 -TATE were obtained in 25 min (n 5 5) with greater than 99% radiochemical purity at high specific activity (.111 GBq [3 Ci]/μmol). 18 F-AMBF 3 -TATE was stable in plasma. PET imaging and biodistribution showed rapid renal excretion with low liver activity. High tumor uptake (10.11% ± 1.67% injected dose/g, n 5 5) was detected at 60 min after injection. Bone uptake was negligible. Tumor-to-liver, tumor-to-blood, tumor-to-muscle, and tumor-to-bone ratios (at 60 min) were 26.2 ± 0.8, 25.1 ± 1.0, 89.0 ± 3.1, and 21.3 ± 3.6, respectively. Conclusion: 18 F-AMBF 3 -TATE was radiolabeled in high yield and at high specific activity, did not require high-performance liquid chromatography purification, exhibited unexpectedly high binding affinity to somatostatin receptor subtype 2, and showed excellent pharmacokinetic properties in vivo, with high tumor uptake and high contrast ratios.