2022
DOI: 10.3390/s22103768
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Rapid Optical Biosensing of SARS-CoV-2 Spike Proteins in Artificial Samples

Abstract: Tests for SARS-CoV-2 are crucial for the mass surveillance of the incidence of infection. The long waiting time for classic nucleic acid test results highlights the importance of developing alternative rapid biosensing methods. Herein, we propose a fiber-optic biolayer interferometry-based biosensor (FO-BLI) to detect SARS-CoV-2 spike proteins, extracellular domain (ECD), and receptor-binding domain (RBD) in artificial samples in 13 min. The FO-BLI biosensor utilized an antibody pair to capture and detect the … Show more

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Cited by 9 publications
(10 citation statements)
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“…The multiplexed FO-BLI biosensor to detect NAbs towards WT and Omicron subvariants in 100-fold diluted sera was summarized in Table S1. Compared to its previous version [6], this biosensor used a more environmentally and user-friendly biomaterial 3-Amino-9-ethylcarbazole (namely, AMEC) as signal enhancer (adopted from our previous study [12]) and the high-salt SD buffer for sample dilution (adopted from another study [13])). Using functionalized fibers, multiple samples or one sample towards multiple subvariants were detected within 6.5-7.5 min (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The multiplexed FO-BLI biosensor to detect NAbs towards WT and Omicron subvariants in 100-fold diluted sera was summarized in Table S1. Compared to its previous version [6], this biosensor used a more environmentally and user-friendly biomaterial 3-Amino-9-ethylcarbazole (namely, AMEC) as signal enhancer (adopted from our previous study [12]) and the high-salt SD buffer for sample dilution (adopted from another study [13])). Using functionalized fibers, multiple samples or one sample towards multiple subvariants were detected within 6.5-7.5 min (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Interferometric biosensors with bilayer configurations reflect light from different material interface interferences, and the interference pattern/light intensity changes due to viral target binding [ 10 , [37] , [38] , [39] ]. They are simple and robust for label-free detection [ 10 ].…”
Section: Optical Biosensors For Multi-virus Detectionmentioning
confidence: 99%
“…The independent detection units miniaturize cross-infection, and multiplexed detection is achieved using a multichannel apparatus. Currently, commercialized multi-channel detection platforms such as the Fortebio [ 38 ] and multichannel electrochemical workstations are available. However, these platforms can only detect several targets simultaneously because of their limited scanning speed and channel amount/space [ 24 , 115 ].…”
Section: Progress Challenges and Future Perspectivesmentioning
confidence: 99%
“…In recent years, some of the investigations have been focused on the design of optical biosensors for the efficient and rapid detection of the SARS-CoV-2 virus. The recognition elements of optical biosensors can be divided into aptamers, molecular imprint polymers (MIPs), and antibodies [ 49 ]. Wenjuan and his colleagues created the first unique microfluidic biosensor using Fresnel reflection for the detection of SARS-CoV-2 without a label that is quick, simple, and sensitive [ 50 ].…”
Section: Sars-cov-2 Biosensorsmentioning
confidence: 99%