Rapid paper disk test for identification of Helicobacter pylori in mixed cultures of gerbil gastric homogenates

Castillo‐Juárez, Israel; Rangel-Vega, Adrián; Romero, Irma

doi:10.1016/j.mimet.2010.07.007

Cited by 1 publication

(1 citation statement)

References 32 publications

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“…The potential H. pylori colonies were subcultured on fresh blood agar plates and incubated for two to four days to obtain monoclonal cultures which were then identified through assays for urease, 23 catalase, 24 and oxidase. 25 Subcultures with positive results for all three enzymatic tests were transferred to a single blood agar plate and incubated for another three to five days. They were then harvested for drug-susceptibility testing and genomic DNA extraction, followed by species confirmation using H. pylori-specific 16S rRNA gene fragment polymerase chain reaction (PCR).…”

Section: Gastric Samplesmentioning

confidence: 99%

Heterogeneity of Helicobacter pylori Strains Isolated from Patients with Gastric Disorders in Guiyang, China

Zhu

et al. 2021

IDR

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Chronic Helicobacter pylori infection causes peptic ulcers in a subpopulation of individuals and is a risk factor for the development of gastric cancer. Multiple infections and heteroresistant H. pylori contribute to poor treatment efficacy. Here, we investigated the extent of genetic diversity among H. pylori strains within a given host and its influence on the results of antibiotic (metronidazole, levofloxacin, clarithromycin, amoxicillin, and tetracycline) susceptibility testing. Materials and Methods: Gastric mucosa biopsy samples were obtained from patients with gastric disorders, including 48 H. pylori positive patients, who were never previously treated for H. pylori infection. Five potential H. pylori colonies isolated from each sample were subcultured for enrichment. Enriched H. pylori colonies were identified through Gram staining and assays for urease, oxidase, and catalase. For each H. pylori monoclonal colony, the antibiotic susceptibility was assessed, genomic DNA was sequenced, and the cytotoxinassociated gene A (cagA) genotype was verified. Co-infection with multiple H. pylori strains was determined using random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR). Results: Thirteen gastric mucosa biopsy samples were positive for H. pylori. Five monoclonal strains isolated from each of these 13 patients were identified as H. pylori. RAPD-PCR indicated that intra-patient monoclonal strains of H. pylori in 10 of the 13 samples exhibited heterogeneity. Among the 13 patients, intra-patient monoclonal strains isolated from 4 patients had identical cagA genotype, whereas intra-patient monoclonal strains isolated from the other 9 patients harbored more than one cagA genotype. The antibiotic susceptibility of five intra-patient monoclonal strains from seven patients was inconsistent. Conclusion: The existence of heterogeneous H. pylori strains with resistance to different drugs and virulence were common within the gastric mucosa of an individual patient.

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Section: Gastric Samplesmentioning

confidence: 99%

Heterogeneity of Helicobacter pylori Strains Isolated from Patients with Gastric Disorders in Guiyang, China

Zhu

et al. 2021

IDR

View full text Add to dashboard Cite

show abstract

Rapid paper disk test for identification of Helicobacter pylori in mixed cultures of gerbil gastric homogenates

Cited by 1 publication

References 32 publications

Heterogeneity of Helicobacter pylori Strains Isolated from Patients with Gastric Disorders in Guiyang, China

Heterogeneity of Helicobacter pylori Strains Isolated from Patients with Gastric Disorders in Guiyang, China

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