Rapid Parlodion Embedding of Temporal Bones

Donahue, D. Joseph; Gussen, Ruth

doi:10.1001/archotol.1966.00760020030009

Cited by 9 publications

(1 citation statement)

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“…The resulting block, measuring about 20 mm 9 20 mm 9 18 mm corresponding to the modiolar spiral axis, was then removed from the container, trimmed, and mounted on a metal chunk, and stored in 70-80% alcohol. 3 The bone was then cut into a thickness of 20 lm horizontally on a sliding microtome. These thin slices of the temporal bone were stored in 80% alcohol.…”

Section: Histological Section Preparation Of Guinea Pig Temporal Bonementioning

confidence: 99%

Registration of Micro-Computed Tomography and Histological Images of the Guinea Pig Cochlea to Construct an Ear Model Using an Iterative Closest Point Algorithm

Lee

Wan

et al. 2010

Ann Biomed Eng

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We present a practical and systematic method to reconstruct accurate physical models of the guinea pig ear (n = 1). The method uses a semi-automatic technique to create three-dimensional (3-D) models of the guinea pig cochlea by registration of micro-computed tomography (CT) and histological images. An iterative closest point algorithm was employed to minimize the sum of square errors with respect to the closest histological model and corresponding micro-CT model. This allowed creation of an accurate geometric ear model including external ear canal, tympanic membrane, middle ear cavity, auditory ossicles, and the cochlea. The characteristic cross-sectional areas of scala tympani, scala vestibuli, and scala media were measured. The length, thickness, and apex width of the guinea pig's basilar membrane were compared to the data found in literature. Some shape parameters were also compared among different species. The results confirmed that the geometric model created by this method was accurate. This method provides an effective way to visualize the 3-D structure and the detailed information about ear geometry required for finite element and multibody dynamic analysis.

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Section: Histological Section Preparation Of Guinea Pig Temporal Bonementioning

confidence: 99%

Registration of Micro-Computed Tomography and Histological Images of the Guinea Pig Cochlea to Construct an Ear Model Using an Iterative Closest Point Algorithm

Lee

Wan

et al. 2010

Ann Biomed Eng

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Articular and internal remodeling in the human otic capsule

Gussen

1968

Am. J. Anat.

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A comprehensive histologic study of the human otic capsule is presented demonstrating the interrelations between cartilage, bone, blood vessels and soft tissue throughout life. Remodeling occurs through continual degeneration of chondrocytes within the uncalcified articular surfaces of the stapediovestibular joint and through continual degeneration of cartilage about the cochlea and semicircular canals. Degenerated chondrocytes are removed by macrophages and new endochondral bone forms from adjacent osteogenically active blood vessels. The cartilage surfaces of the footplate and oval window are constantly replenished by new cartilage formed by mesenchymal cells of the annular ligament. Within the otic capsule, new cartilage forms from mesenchymal cells lining the labyrinth. Cartilage foci continually undergo partial replacement by endochondral bone with remnants of uncalcified cartilage matrix remaining, forming globuli ossei and interglobular spaces. In addition to continual endochondral bone formation partially replacing the continually forming and degenerating cartilage, chondroid bone forms by direct transformation of some cartilage to bone. These chondroid bone areas are cellular in young age groups, but become pale and relatively acellular with age. These processes occur throughout life, regardless of age or sex.

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