Rapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum)

Somatic Embryogenesis on Plumule and Radicle Explants Obtained from Warm Water Hydroprimed Wheat (Triticum aestivum L.) cv. Kunduru and cv. Cakmak Seeds

“…A review of previous literature suggests variable success in tissue culture of different wheat species using callus of immature embryos (Becker et al 1994;Elena & Ginzo 1988;Maddock et al 1983;Weeks et al 1993) and mature embryos (Bi et al 2007;Patnaik et al 2006). Both plumule and radicle tissue initiated callus induction are affected by types of explant and plant species used in the study at all concentrations of 2,4-D. Callus weight and gain was cultivar and explant specific and largely inconsistent.…”

“…Development of transformation techniques has become one of the focal issues in wheat tissue culture and is becoming an important vehicle in wheat breeding programs throughout the world. It is making use of development of haploids, organogenesis, somatic embryogenesis, selection of somaclonal variants and production of transgenic plants for wheat improvement (Becker et al 1994;Bi et al 2007;Chu et al 1990;Elena & Ginzo 1988;Fellers et al 1995;Maddock et al 1983;Patnaik et al 2006;Rajyalakshmi et al 1991;Sarker & Biswas 2002;Weeks et al 1993). Previous reports suggested the effects of culture medium and its supplements (Mathias & Simpson 1986) tissue (Vasil 1994) and genotype (Hess & Carman 1998) on tissue culture of wheat.…”

Production of herbicide-resistant cowpea (Vigna unguiculata L.) transformed with the bar gene

“…The surviving plants further subjected to Basta spray revealed the presence of bar gene in transformed plants. Many researchers have reported the tolerance of crop plants to phosphinothricin after the successful transformation of the bar gene in crops like maize (Gordon-Kamm et al, 1990), rice (Cao et al, 1992), wheat (Weeks et al, 1993), sugarcane (Manickavasagam et al, 2004), and cowpea (Ilori et al, 2011).…”

“…Phosphinothricin N-acetyltransferase encodes phosphinothricin, the active ingredient of herbicides such as Basta®, by acetylation. The bar gene has been widely used as an effective selectable marker in many crop species like maize (Gordon-Kamm et al, 1990), rice (Cao et al, 1992), wheat (Weeks et al, 1993), sugarcane (Manickavasagam et al, 2004), and cowpea (Ilori et al, 2011).…”

TRANSGENIC EGYPTIAN WHEAT (Triticum aestivum L.) WITH Arabidopsis NPR1 GENE via BIOLISTIC BOMBARDMENT

“…Immature embryos with scutellum side up were cultured on callus induction medium (CIM) (Weeks et al, 1993) containing Murashige and Skoog salts (Murashige and Skoog, 1962) supplemented with 0.15 g of L-Asparagine, 0.1 g of myo-inositol, 20 g sucrose, 2.5 g Phytagel as a solidifying agent and 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) as an auxin source. Then embryos were incubated in dark for five days before bombardment with gold particles coated with DNA.…”