Rapid profiling of the infection of Bacillus anthracis on human macrophages using SELDI-TOF mass spectroscopy

Seo, Gwi-Moon; Kim, Seong-Joo; Chai, Young Gyu

doi:10.1016/j.bbrc.2004.10.146

Cited by 9 publications

(4 citation statements)

References 10 publications

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“…Macrophages are the immune cells in hosts encountering anthrax spores and germinating the bacteria [8][9][10][11] and thus there have been many studies about spore infection and LeTx toxicity in macrophages 5,[12][13][14][15][16] . We focused the present study on the gene expression patterns involved in host-pathogen interactions using genomic analysis 14 studied the gene expression profile of a murine macrophage cell line (Raw 264.7) treated with LeTx (2 μg/mL of PA, 2 μg/mL of LF) using the Atlas TM 1.5 membrane array system (1,185 genes).…”

Section: Abstract Anthrax Lethal Toxin Macrophages Pten Gsk-3βmentioning

confidence: 99%

Early response in macrophages by exposure to a low concentration of anthrax lethal toxin

Jung

Nam

Kim

et al. 2011

Mol. Cell. Toxicol.

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Section: Abstract Anthrax Lethal Toxin Macrophages Pten Gsk-3βmentioning

confidence: 99%

Early response in macrophages by exposure to a low concentration of anthrax lethal toxin

Jung

Nam

Kim

et al. 2011

Mol. Cell. Toxicol.

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“…It is an affinity-based MS method in which proteins are selectively adsorbed onto a chemically or biochemically modified surface. Seo, Kim, and Chai (2004) studied the protein-expression profile in human macrophages that were infected by B. anthracis spores with SELDI-TOFMS. Lundquist et al (2005) demonstrated that SELDI-TOFMS can generate unique and reproducible protein profiles for F. tularensis subspecies, to allow its subspecies to be distinguished from each other.…”

Section: Seldi-msmentioning

confidence: 99%

Advances in mass spectrometry for the identification of pathogens

Reddy

2011

Mass Spectrometry Reviews

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Mass spectrometry (MS) has become an important technique to identify microbial biomarkers. The rapid and accurate MS identification of microorganisms without any extensive pretreatment of samples is now possible. This review summarizes MS methods that are currently utilized in microbial analyses. Affinity methods are effective to clean, enrich, and investigate microorganisms from complex matrices. Functionalized magnetic nanoparticles might concentrate traces of target microorganisms from sample solutions. Therefore, nanoparticle-based techniques have a favorable detection limit. MS coupled with various chromatographic techniques, such as liquid chromatography and capillary electrophoresis, reduces the complexity of microbial biomarkers and yields reliable results. The direct analysis of whole pathogenic microbial cells with matrix-assisted laser desorption/ionization MS without sample separation reveals specific biomarkers for taxonomy, and has the advantages of simplicity, rapidity, and high-throughput measurements. The MS detection of polymerase chain reaction (PCR)-amplified microbial nucleic acids provides an alternative to biomarker analysis. This review will conclude with some current applications of MS in the identification of pathogens.

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“…MALDI-TOF-MS). This work experimentally focuses on real-time PCR [23] and MALDI-TOF-MS [24] for the analysis of bacterial spores after inactivation. In case of a biological attack with BWA, identification times are critical for the medical treatment of exposed people [25].…”

Section: Introductionmentioning

confidence: 99%

Effects of inactivation methods on the analysis of Bacillus atrophaeus endospores using real‐time PCR and MALDI‐TOF‐MS

Talbot

Russmann²,

Köhne³

et al. 2010

Engineering in Life Sciences

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A wide range of analytical methods are available for the detection and identification of biological warfare agents. These technologies are often hampered in their performance when the inactivated samples are analyzed. To work with pathogens outside of biosafety level 3 laboratories, a complete inactivation is mandatory when appropriate protection equipment is unavailable. When methods of inactivation are used, the detection of bacteria becomes more difficult. In contrast to measuring viable organisms, inactivation steps can have a massive impact on the intrinsic cellular information. This study examined the effects of autoclaving and chemical inactivation methods on Bacillus spores using biological warfare detection setups like real‐time PCR and MALDI‐TOF‐MS. Here, the inactivation of Bacillus atrophaeus spores with formaldehyde, which is a suggested model for biological warfare spore agents, was compared with other inactivation reagents like Wofasteril®E400, a commercially available decontaminant based on peroxyacetic acid. With Wofasteril®E400 the critical factor of inactivation time was reduced to about 15 min and a limit of detection of 8500 spores by PCR was still measurable using five‐times‐washed spores. It has also been shown that MALDI‐TOF‐MS peak information can be hampered by inactivation methods.

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Rapid profiling of the infection of Bacillus anthracis on human macrophages using SELDI-TOF mass spectroscopy

Cited by 9 publications

References 10 publications

Early response in macrophages by exposure to a low concentration of anthrax lethal toxin

Early response in macrophages by exposure to a low concentration of anthrax lethal toxin

Advances in mass spectrometry for the identification of pathogens

Effects of inactivation methods on the analysis of Bacillus atrophaeus endospores using real‐time PCR and MALDI‐TOF‐MS

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