Rapid protein separations in ultra-short microchannels: microchip sodium dodecyl sulfate–polyacrylamide gel electrophoresis and isoelectric focusing

Han, Jongyoon; Singh, Anup K.

doi:10.1016/j.chroma.2004.08.018

Cited by 43 publications

(46 citation statements)

References 25 publications

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“…However, a growing number of research groups perform CIEF on chips [81,146,147]. Recently, whole-column detection was introduced to CIEF [21,94,97,148,149], which allows monitoring dynamics of CIEF.…”

Section: Ciefmentioning

confidence: 99%

Capillary electrophoresis of proteins 2003–2005

Dolnı́k¹

2006

Electrophoresis

139

117

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This review article with 304 references describes recent developments in CE of proteins, and covers the two years since the previous review (Hutterer, K., Dolník, V., Electrophoresis 2003, 24, 3998-4012) through Spring 2005. It covers topics related to CE of proteins, including modeling of the electrophoretic migration of proteins, sample pretreatment, wall coatings, improving separation, various forms of detection, special electrophoretic techniques such as affinity CE, CIEF, and applications of CE to the analysis of proteins in real-world samples including human body fluids, food and agricultural samples, protein pharmaceuticals, and recombinant protein preparations.

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Section: Ciefmentioning

confidence: 99%

Capillary electrophoresis of proteins 2003–2005

Dolnı́k¹

2006

Electrophoresis

139

117

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“…Some attempt has been done to achieve this goal, Han et al 95) has introduced chip for SDS separation where the separation could be achieved in less than 2 mm within 30 s using polyacrylamide gel as a matrix for protein molecules ranging from 20 to 200 kDa. While IEF was achieved within tens of seconds in mm-length channel Li et al 96) has proposed a comprehensive chip-alternative for conventional 2DE separation.…”

Section: Coupling Ief With Sds On Chipmentioning

confidence: 99%

Low Viscous Separation Media for Genomics and Proteomics Analysis on Microchip Electrophoresis System

Jabasini

Murakami

Kaji

et al. 2006

Biological & Pharmaceutical Bulletin

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Microchip electrophoresis has widely grown during the past few years, and it has showed a significant result as a strong separation tool for genomic as well as proteomic researches. To enhance and expand the role of microchip electrophoresis, several studies have been proposed especially for the low viscous separation media, which is an important factor for the success of microchip with its narrow separation channels. In this paper we show an overview for the done researches in the field of low viscous media developed for the use in microchip electrophoresis. For genomic separation studies polyhydroxy additives have been used enhance the separation of DNA at low polymer concentration of HPMC (Hydroxypropylmethyl cellulose) which could keep the viscosity low. Mixtures of poly(ethylene oxide) as well as Hydroxyporpyl cellulose have been successfully introduced for chip separation. Furthermore high molecular mass polyacrylamides at low concentrations have been studied for DNA separation. A mixture of polymer nanoparticle with conventional polymers could show a better resolution for DNA at low concentration of the polymer. For the proteomic field isoelectric focusing on chip has been well overviewed since it is the most viscous separation media which is well used for the protein separation. The different types of isoelectric focusing such as the ampholyte-free type, the thermal type as well as the ampholyte-depended type have been introduced in this paper. Isoelectric focusing on chip with its combination with sodium dodecyl sulfate (SDS) page or free solution could give a better separation. Several application for this low viscous separation medias for either genomic or proteomic could clearly show the importance of this field.

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“…In conventional CIEF, however, the focused analytes must be transported toward a detection window, which can sometimes decrease the resolution due to a band broadening during transportation [5]. Recently, many IEF techniques using microfluidic devices (microfluidic IEF, MIEF) have been developed to overcome this drawback [6][7][8][9][10][11][12]. MIEF has many advantages compared to CIEF, including a short analysis time (within 5 min) due to a short separation channel (several cm) and the requirement of a minimal amount of reagents and samples (several L).…”

Section: Introductionmentioning

confidence: 99%

“…MIEF has many advantages compared to CIEF, including a short analysis time (within 5 min) due to a short separation channel (several cm) and the requirement of a minimal amount of reagents and samples (several L). In particular, a short separation length in MIEF makes whole-column imaging possible, which enables real-time observation of an IEF process over the whole length of the microchannel without the requirement of any of the transportation processes necessary for conventional CIEF [9][10][11][12]. More recently, a microfluidic device for two-dimensional electrophoresis was independently developed by Kim and Moon and Yang et al to obtain a higher resolution for analyzing a complicated mixture of biogenic samples [13,14].…”

Section: Introductionmentioning

confidence: 99%

A Simple and Easy-to-Use Capillary Isoelectric Focusing Technique Using Reagent-Release Hydrogels

et al. 2017

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Reagent-release hydrogels containing an acid or a base solution were prepared and employed as ion suppliers in isoelectric focusing using short capillaries. In conventional isoelectric focusing techniques using microfluidic channels, complicated liquid manipulations are required, which can decrease analytical performance such as the reproducibility of a focusing position. To develop a rapid and sensitive assay based on microfluidic isoelectric focusing, we previously prepared reagent-release capillaries retaining ampholytes by physical adsorption on the inner surface of a short capillary, which were then applied to isoelectric focusing. In this study, reagent-release hydrogels containing an acid or a base solution were developed for isoelectric focusing using short capillaries in place of the acid and base solutions used in conventional microfluidic isoelectric focusing. The prepared reagent-release hydrogels showed some advantages for isoelectric focusing, e.g., high durability for at least five weeks of storage, easy handling as compared to the conventional solutions, and suppression of hydrodynamic flow in capillaries, which often decreases the reproducibility in capillary isoelectric focusing. The migration behavior of ions from the hydrogels into the capillary and the formation of a pH gradient in isoelectric focusing using the reagent-release hydrogels were investigated, which confirmed that the prepared reagent-release hydrogels could form a similar pH gradient to that obtained with conventional capillary isoelectric focusing using the solutions.

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Rapid protein separations in ultra-short microchannels: microchip sodium dodecyl sulfate–polyacrylamide gel electrophoresis and isoelectric focusing

Cited by 43 publications

References 25 publications

Capillary electrophoresis of proteins 2003–2005

Capillary electrophoresis of proteins 2003–2005

Low Viscous Separation Media for Genomics and Proteomics Analysis on Microchip Electrophoresis System

A Simple and Easy-to-Use Capillary Isoelectric Focusing Technique Using Reagent-Release Hydrogels

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