Rapid prototyping of multi-scale biomedical microdevices by combining additive manufacturing technologies

Abstract

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Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility

“…The use of ultrahigh precision additive manufacturing technologies, especially direct laser writing based on two-photon polymerization [50,51], may help to further reduce the size of the proposed microsystem down to 10 µm-wide channels and surface roughness of around 1-5 µm, as we have already verified [42]. Such size reduction may help with trying to obtain a single row of cells crawling along the channels to obtain a more ideal single cell behavior and to assess the effect of more subtle topographies on cell response.…”

“…to the other one, so as to promote or prevent cell movement from one chamber to another. The design presented here is inspired by previous microsystems aimed at interacting with cells [40][41][42], although using better suited scales for interacting at the single-cell level without using ultra-high precision manipulators. In short, the overall structure, which mainly comprises the different walls of the two pools and the six microchannels, has been designed using conventional 3D computer-aided design methods.…”

Microstructured Devices for Studying Cell Adhesion, Dynamics and Overall Mechanobiology

“…Multi-scale approaches are also possible, by combining different prototyping technologies, a more conventional 3D printer for the larger details and 2PP for the tiniest features (Hengsbach and Díaz Lantada 2014). …”

Monolithic 3D labs- and organs-on-chips obtained by lithography-based ceramic manufacture

“…This monolithic approach is of interest, as recently highlighted [4][5][6], especially for handling fluids, as leakage is prevented thanks to the promotion of device integration, to the reduction of components and to the consequent elimination of joints between parts. As perceived from the aforementioned references, there is a growing concern towards more integrated design and manufacturing procedures linked to these engineering systems.…”