Rapid purification of uricase from Bacillus fastidiosus

Abstract

A simple, rapid procedure was developed for the purification of uricase from Bacillus fustidiosus. The enzyme was purified to homogeneity in a two-step procedure with the aid of fast-flow column chromatography. In this way high yields (37 %) of pure uricase with a specific activity of 78.3 U/mg were obtained in a short time.

Trends in industrial and environmental biotechnology research in Tanzania

“…There have been efforts to isolate bacteria and screen fungi whose enzymes are of medicinal, industrial and environmental importance with the chief aim of isolating and characterizing them (Muruke et al, 1993(Muruke et al, , 1995(Muruke et al, , 1996Akhmanova et al, 1998). Growth of Bacillus fastidiosus in a medium containing glycerol as a carbon source and urea as a nitrogen source leads to production of glycerol kinase, glycerol 3-phosphate, dehydrogenase, allantoinase, urease and carboligase under batch and continuous culture conditions.…”

“…Growth of Bacillus fastidiosus in a medium containing glycerol as a carbon source and urea as a nitrogen source leads to production of glycerol kinase, glycerol 3-phosphate, dehydrogenase, allantoinase, urease and carboligase under batch and continuous culture conditions. Considering that uricase is widely used in the determination of urate levels in blood and urine for treatment of gout and hyperuricaemia; and also for commercial formulations of hair colouring, an attempt has been made to purify uricase from B. fastidiosus in a two-step procedure with the aid of fastflow column chromatography (Muruke et al, 1996). In this way, high yields (37%) of pure uricase with a specific activity of 78.3 U/mg are obtainable in a short time.…”

Biotechnology research

“…Research on production and purification of enzymes of economic importance has drawn a lot of interest. There have been efforts to isolate bacteria and screen fungi whose enzymes are of medicinal, industrial and environmental importance with the chief aim of isolating and characterizing them (Muruke et al, 1993(Muruke et al, , 1995(Muruke et al, , 1996Akhmanova et al, 1998). Growth of Bacillus fastidiosus in a medium containing glycerol as a carbon source and urea as a nitrogen source leads to production of glycerol kinase, glycerol 3-phosphate, dehydrogenase, allantoinase, urease and carboligase under batch and continuous culture conditions.…”