Rapid quantification of bacterial cells in potable water using a simplified microfluidic device

Sakamoto, Chieko; Yamaguchi, Naohito; Yamada, Masao; Nagase, Hirotsugu; Seki, Minoru; Nasu, Masao

doi:10.1016/j.mimet.2006.11.003

Cited by 36 publications

(37 citation statements)

References 27 publications

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“…uidaho.edu/) and the result was "uncultured bacterium." More than 90% of bacteria in aquatic environments are difficult to culture under conventional conditions [1][2][3] and this result was convincing.…”

Section: Bacterial Community Profiling In Hydroponics Solutionmentioning

confidence: 93%

“…Total bacterial number of a targeted freshwater sample can be first determined by a microchip-based system for rapid quantification of bacteria in freshwater. 2,3) In addition, the bacterial community structure should be analyzed by a microchip-based system for DNA extraction, PCR, and on-chip T-RFLP to determine whether harmful bacteria are increasing when total bacterial number is increasing.…”

Section: Bacterial Community Profiling In Hydroponics Solutionmentioning

confidence: 99%

“…Microchip-based systems have been developed for rapid quantification of bacteria in freshwater. 2,3) These systems enable counting of bacteria in freshwater within 1 h using a microfluidic device following fluorescent staining, without concentration or other time-consuming preparation steps.…”

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confidence: 99%

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Microchip-Based Terminal Restriction Fragment Length Polymorphism for On-Site Analysis of Bacterial Communities in Freshwater

Yamaguchi

Matsukawa

Shintome

et al. 2013

Biological & Pharmaceutical Bulletin

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Assessing microbiological quality assurance by monitoring bacteria in various sources of freshwater used for human consumption, recreation, and food preparation is important for a healthy life. Bacterial number and their community structure in freshwater should be determined as quickly as possible, and "realtime" and "on-site" microbiological methods are required. In this study, we examined the protocol for microchip-based terminal restriction fragment length polymorphism (T-RFLP) analysis, which uses microchip electrophoresis for rapid microbial community analysis. The availability of microchip-based T-RFLP was compared with conventional T-RFLP analysis, which uses a capillary electrophoresis system, with freshwater samples (spring water, river water, groundwater, and hydroponics solution). The detection limit of targeted bacteria by on-chip T-RFLP analysis was 1% (10 3 cells/mL). The fragment sizes determined by the two analysis methods were highly correlated (r 2 =0.98). On-chip T-RFLP analysis was completed within 15 min. T-RFLP profiles of nine hydroponics solution samples were analyzed by multidimensional scaling. Considerable changes and stability in bacterial community structure during hydroponic culture were detected by both analyses. These results show that on-chip T-RFLP analysis can monitor changes in bacterial community structure, as well as conventional T-RFLP analysis. The present results indicate that on-chip T-RFLP analysis is an effective tool for rapid and "on-site" bacterial community profiling in freshwater environments, as well as freshwater used for medical and industrial purposes.

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Section: Bacterial Community Profiling In Hydroponics Solutionmentioning

confidence: 93%

Section: Bacterial Community Profiling In Hydroponics Solutionmentioning

confidence: 99%

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Microchip-Based Terminal Restriction Fragment Length Polymorphism for On-Site Analysis of Bacterial Communities in Freshwater

Yamaguchi

Matsukawa

Shintome

et al. 2013

Biological & Pharmaceutical Bulletin

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“…In fact, this technological platform allows obtaining significant results on over expanding fields, going from medicine 1,2 to biology, and from environmental monitoring 3 to aerospace applications. 4 The development of more complex and effective microfluidic devices strictly depends on the possibility to address challenging needs, such as miniaturized and light devices, low consumption of samples and reagents, complex sample preparation, short time-to-result, and simple readout.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration

et al. 2015

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The flow focusing is a fundamental prior step in order to sort, analyze, and detect particles or cells. The standard hydrodynamic approach requires two fluids to be injected into the microfluidic device: one containing the sample and the other one, called the sheath fluid, allows squeezing the sample fluid into a narrow stream. The major drawback of this approach is the high complexity of the layout for microfluidic devices when parallel streams are required. In this work, we present a novel parallelized microfluidic device that enables hydrodynamic focusing in each microchannel using a single feed flow. At each of the parallel channels, a cross-filter region is present that allows removing fluid from the sample fluid. This fluid is used to create local sheath fluids that hydrodynamically pinch the sample fluid. The great advantage of the proposed device is that, since only one inlet is needed, multiple parallel micro-channels can be easily introduced into the design. In the paper, the design method is described and the numerical simulations performed to define the optimal design are summarized. Moreover, the operational functionality of devices tested by using both polystyrene beads and Acute Lymphoid Leukemia cells are shown. V C 2015 AIP Publishing LLC.

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“…Microfluidic chip-based devices have been employed for many analyses, such as capillary electrophoresis, 10) PCR, 11) and flow cytometry (on-chip flow cytometry). 12) We have applied commercially available on-chip flow cytometry to analyze hybridization results by beads assay for the detection of foodpoisoning bacteria. 13) On-chip flow cytometry has also been applied for the enumeration of freshwater bacteria.…”

Section: Introductionmentioning

confidence: 99%

Rapid On-chip flow Cytometric Detection of Listeria monocytogenes in Milk

Ikeda

Yamaguchi

Nasu

2009

JOURNAL OF HEALTH SCIENCE

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Rapid quantification of bacterial cells in potable water using a simplified microfluidic device

Cited by 36 publications

References 27 publications

Microchip-Based Terminal Restriction Fragment Length Polymorphism for On-Site Analysis of Bacterial Communities in Freshwater

Microchip-Based Terminal Restriction Fragment Length Polymorphism for On-Site Analysis of Bacterial Communities in Freshwater

Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration

Rapid On-chip flow Cytometric Detection of Listeria monocytogenes in Milk

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