Rapid quantification of cyanamide by ultra-high-pressure liquid chromatography in fertilizer, soil or plant samples

Nagumo, Yoshifumi; Tanaka, Kohei; Tewari, Kaushal; Thiraporn, K.; Tsuchida, Toru; Honma, Toshimitsu; Ohtake, Norikuni; Sueyoshi, Kuni; Ohyama, Takuji

doi:10.1016/j.chroma.2009.05.067

Cited by 14 publications

(15 citation statements)

References 5 publications

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“…The derivatization of amino acids and amino thiols was accomplished by following the Waters AccQ Tag TM Ultra UHPLC amino analysis procedures, as described by Nagumo et al [26] and Salazar et al [25]. Briefly, 350 µl of borate derivatization buffer (0.2 M sodium borate, pH 8.8, with 5 mM calcium disodium EDTA), 50 µl of amino acid standard or jujube extract, and 100 µl of reconstituted AQC (10 mM AQC dry powder in acetonitrile) were placed in a 2-ml propylene vial [27,28].…”

Section: Amino Acidsmentioning

confidence: 99%

Effects of water deficit during maturation on amino acids and jujube fruit eating quality

Collado‐González

Cruz

Medina

et al. 2014

Maced. J. Chem. Chem. Eng.

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<p>Pear-jujube yield and fruit characteristics can be clearly modified by a water deficit imposed during fruit maturation period. One essential (cystine (Cys-cys)) and seven non-essential (4-hydroxyproline (p-Hyp), α-aminoadipic acid (AADA), ornithine (orn), β-aminoisobutyric acid (BAIB), α-amino-n-butyric acid (AABA), cystathionine (Cysta) and homocystine (Hcys-cys)) amino acids were identified for the first time. Fruits from plants exposed to a moderate water deficit during the maturation stage (T1) initiated the ripening phase earlier than control (T0) fruits and showed an improved eating quality. Fruits resulting from severe water deficit (T2) showed changes in their physical characteristics and reached more advanced degree of ripening than T0 and T1 fruits, improving not only most of fruit chemical characteristics that make up the taste but also the nutritional value. The decrease in the asparagine (Asn) content of the fruit as a result of severe water deficit is a positive aspect, which prevents acrylamide formation during heat-processing of the fruit.</p>

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Section: Amino Acidsmentioning

confidence: 99%

Effects of water deficit during maturation on amino acids and jujube fruit eating quality

Collado‐González

Cruz

Medina

et al. 2014

Maced. J. Chem. Chem. Eng.

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“…However, because of chromatographic separation problems and detectability, cyanamide has to be derivatized prior to separation and quantification by analytical system. The 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate was applied for analyses of cyanamide using HPLC with ultraviolet [9] or fluorescence detection [12]. Other derivatizing reagents used for amino acid analyses are sometimes applied to derivatization of cyanamide, namely, succinimidyl esters.…”

Section: Introductionmentioning

confidence: 99%

“…Cyanamide is highly toxic and causes adverse health effects to mammals, including severe irritation of the eyes, skin, and respiratory system, miosis, salivation, lacrimation, and twitching [9]. The Commission of the European Communities decided the noninclusion of cyanamide in Annex I to Council Directive 91/ 414/EEC because of its toxicity [10].…”

Section: Introductionmentioning

confidence: 99%

“…Other derivatizing reagents used for amino acid analyses are sometimes applied to derivatization of cyanamide, namely, succinimidyl esters. The 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate was applied for analyses of cyanamide using HPLC with ultraviolet [9] or fluorescence detection [12]. Other studies have used 2,6-dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester (DMQC-OSu) as derivatizing reagent for aliphatic amines, showing a high sensitivity [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…The traditional sample preparation methods for preconcentration and clean-up for cyanamide analyses include extraction with water and purification using a column containing diatomaceous earth [12], extraction with 2% acetic acid [9], evaporation to dryness under nitrogen steam [3], and purification using a normal-phase extraction column [1]. Microextraction methods, namely, dispersive liquid-liquid microextraction (DLLME), have attracted much attention in recent years as alternatives for classic procedures [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Response surface methodology for optimization of cyanamide analysis by in situ derivatization and dispersive liquid–liquid microextraction

Neves

Melo

Pinho

et al. 2014

Journal of Chemometrics

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Cyanamide is widely used for agricultural purposes; therefore, its residues can be found in water. A new method was developed for its quantification using in situ derivatization with 2,6-dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester followed by dispersive liquid-liquid microextraction (DLLME) and high-performance liquid chromatography/fluorescence analysis. Multivariate chemometric techniques were successfully used to obtain the optimum conditions for direct derivatization and DLLME extraction. Derivatization parameters and DLLME extraction conditions were optimized by a two-step design, 2 k factorial design for screening, and central composite design for optimization. Best derivatization conditions were addition of 600 μL of derivatizing reagent, a temperature of 4 ºC, and pH 8.5, whereas for optimum extraction 800 μL of solvent, 30% NaCl conc. w/v, and pH 3.8 were chosen. The analytical performance of the method for routine analysis was evaluated. Excellent linearity was achieved from 10 to 200 μg L À1 with a correlation factor of 0.9996. Precision ranged from 3.5% to 5.5% for intraday assays and 8.5% to 8.6% for interday assays. The mean recoveries performed on water from different origins (ground, river, sea, tap, and mineral) at three levels of concentration (20, 75, and 200 μg L À1 ) ranged from 90.2% to 110.2%.Abbreviations: DMQC-OSu, 2,6-dimethyl-4-quinolinecarboxylic acid Nhydroxysuccinimide; DLLME, dispersive liquid-liquid microextraction; CCD, central composite design; IS, internal standard.

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Determining Hydrogen Cyanamide in Fruit by Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate and HPLC with Fluorescence Detection

Cai

Zhao

et al. 2012

Chromatographia

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Rapid quantification of cyanamide by ultra-high-pressure liquid chromatography in fertilizer, soil or plant samples

Cited by 14 publications

References 5 publications

Effects of water deficit during maturation on amino acids and jujube fruit eating quality

Effects of water deficit during maturation on amino acids and jujube fruit eating quality

Response surface methodology for optimization of cyanamide analysis by in situ derivatization and dispersive liquid–liquid microextraction

Determining Hydrogen Cyanamide in Fruit by Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate and HPLC with Fluorescence Detection

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