Rapid quantification of single-nucleotide mutations in mixed influenza A viral populations using allele-specific mixture analysis

Effect of Oseltamivir Carboxylate Consumption on Emergence of Drug-Resistant H5N2 Avian Influenza Virus in Mallard Ducks

“…Other recent research has utilized quantitative real-time or real-time RT-PCR coupled with mathematical models based on differences in Ct values between wild-type and resistant strains for comparison. This can be extremely sensitive for detecting very low levels of a specific mutation (19,37,(54)(55)(56). While we based our site-specific RT-PCR on these models, which correlated with our Sanger sequencing results plus detected one additional sample that Sanger sequencing missed, we recognize that there is a different level of sensitivity between these methods.…”

Emerging oseltamivir resistance in seasonal and pandemic influenza A/H1N1

“…17,18 Assays targeting the H275Y mutation for seasonal or pandemic H1N1 are summarized in Table 2. [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] Comparative data evaluating various PCR genotyping protocols are not readily available, but most assays are highly sensitive. Some can detect minor variant populations of 0.5% with accurate and reproducible quantification.…”

Diagnostic accuracy of an allele-specific reverse transcriptase-PCR assay targeting the H275Y oseltamivir resistant mutation in 2009 pandemic influenza A/H1N1 virus

“…Reverse transcriptase (RT)-PCR protocols have been described for detection of the H275Y mutation in seasonal H1N1 influenza and avian H5N1 influenza but few have been reported for pH1N1 virus. [19][20][21][22][23] Molecular detection of H275Y mutation by RT-PCR in diagnostic laboratories must be robust, rapid and inexpensive.…”