Rapid quantitation and identification of the chemical constituents in Danhong Injection by liquid chromatography coupled with orbitrap mass spectrometry

Xu, Lulu; Shang, Zhanpeng; Tao, Bo; Sun, Lei; Guo, Qilei; Qiao, Xue; Ye, Min

doi:10.1016/j.chroma.2019.460378

Cited by 27 publications

(19 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Danhong injection (DHI), as one of the most popular TCM medications for the treatment of cardiovascular and cerebrovascular diseases, is a standardized product extracted from Salvia miltiorrhiza Bunge and Carthamus tinctorius L., and the dose ratio of raw material is 3:1 (Wan et al, 2018;Feng et al, 2019). The major representative components in DHI are danshensu, salvianic acid, 3, 4-dihydroxybenzaldehyde, protocatechuic aldehyde, and rosmarinic acid; these compounds are related to pharmacological effects of DHI (Li et al, 2019;Xu et al, 2019). DHI has been used extensively in the clinical treatment of coronary heart disease and acutely cerebral infarction for many years (He et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Danhong Injection Attenuates Cerebral Ischemia-Reperfusion Injury in Rats Through the Suppression of the Neuroinflammation

Yingjie

Zhao

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Neuroinflammation is one of the major causes of damage of the central nervous system (CNS) and plays a vital role in the pathogenesis of cerebral ischemia, which can result in long-term disability and neuronal death. Danhong injection (DHI), a traditional Chinese medicine injection, has been applied to the clinical treatment of cerebral stoke for many years. In this study, we investigated the protective effects of DHI on cerebral ischemia-reperfusion injury (CIRI) in rats and explored its potential anti-neuroinflammatory properties. CIRI in adult male SD rats was induced by middle cerebral artery occlusion (MCAO) for 1 h and reperfusion for 24 h. Results showed that DHI (0.5, 1, and 2 ml/kg) dose-dependently improved the neurological deficits and alleviated cerebral infarct volume and histopathological damage of the cerebral cortex caused by CIRI. Moreover, DHI (0.5, 1, and 2 ml/kg) inhibited the mRNA expressions of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), intercellular cell adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) in ischemic brains, downregulated TNF-α, IL-1β, and monocyte chemotactic protein-1 (MCP-1) levels in serum, and reduced the neutrophil infiltration (myeloperoxidase, MPO) in ischemic brains, in a dose-dependent manner. Immunohistochemical staining results also revealed that DHI dose-dependently diminished the protein expressions of ICAM-1 and COX-2, and suppressed the activation of microglia (ionized calcium-binding adapter molecule 1, Iba-1) and astrocyte (glial fibrillary acidic protein, GFAP) in the cerebral cortex. Western blot analysis showed that DHI significantly downregulated the phosphorylation levels of the proteins in nuclear factor κB (NF-κB) and mitogen-activated protein kinas (MAPK) signaling pathways in ischemic brains. These results indicate that DHI exerts anti-neuroinflammatory effects against CIRI, which contribute to the amelioration of CNS damage.

show abstract

Section: Introductionmentioning

confidence: 99%

Danhong Injection Attenuates Cerebral Ischemia-Reperfusion Injury in Rats Through the Suppression of the Neuroinflammation

Yingjie

Zhao

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…In its secondary mass spectrum (Figure 5A), excimer ion [M‐H] – loses one molecule of CO 2 (44 Da) to generate m / z 153.0453 fragment ions, and excimer ion [M‐H] – loses one molecule of H 2 O (18 Da) to generate fragment ion at m / z 179.0331 and continue to remove a molecule of CO 2 (44 Da) to generate fragment ion at m / z 135.1045. It is presumed to be Danshensu, and its mass fragmentation pattern is shown in Figure 5B [26–29]. According to the fragment ions of these four compounds, m / z 179 ([C 10 H 11 O 3 ] – or [C 9 H 7 O 4 ] – ) is the characteristic fragment ion of penylpropanoids.…”

Section: Resultsmentioning

confidence: 99%

Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Wang

et al. 2020

J of Separation Science

View full text Add to dashboard Cite

Duzhong Jiangya tablet is a hypotensive drug. In this study, high-performance liquid chromatography-Fourier transform-ion cyclotron resonance-mass spectrometry technology was used to quickly identify its chemical composition. SinoChrom ODS-BP column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile(A)-0.1% formic acid solution(B). The flow rate was 1 mL/min. Extracted ion chromatogram was used to analyze the samples in positive and negative ion modes. Based on the accurate mass spectrometry information (such as quasi-molecular ions and fragment ions) obtained from the instrument, combined with reference compounds and literature, the chemical composition of Duzhong Jiangya Tablets was identified. A total of 131 compounds were identified, including four types of penylpropanoids, six types of phenylethanoid glycosides, 10 types of organic acids, 14 types of iridoids, 12 types of lignans, 18 types of alkaloids, seven types of coumarins, and 60 kinds of flavonoids. This established method can quickly and efficiently identify chemical constituents in Duzhong Jiangya tablet, lay a foundation for the research on the efficacy and quality of this traditional Chinese medicine, and provide a reference for the characterization of the chemical constituents of other traditional Chinese medicine preparations. K E Y W O R D S chemical composition, Duzhong Jiangya Tablet, Fourier transform ion cyclotron resonance, traditional Chinese medicine 1 INTRODUCTION Varieties of medicinal materials in traditional Chinese medicine compound preparations have complex chemical components. The synergy between the chemical components is the material basis for the traditional Chinese medicine preparations to exert their pharmaco-Article Related Abbreviations: ICR, ion cyclotron resonance logical effects [1]. The active ingredients of most Chinese medicines are still unknown, although there are many reports on Chinese medicine research. Therefore, it is very important to establish a fast and efficient method to identify the chemical components in traditional Chinese medicine. This can reasonably explain the material basis of the efficacy of traditional Chinese medicine. Duzhong Jiangya Tablet consists of Eucommia ulmoides Oliv, Leonurus japonicus Houtt, Prunella vulgaris L,

show abstract

“…After deleting duplicate data, a total of 12 major compounds were collected as candidate compounds (Table 1) [52]. And all of the chemical constituents of DHI in Table S1.…”

Section: Compound-putative Target Networkmentioning

confidence: 99%

Investigating the multi-target pharmacological mechanism of danhong injection acting on unstable angina by combined network pharmacology and molecular docking

Guo

Zhou

et al. 2020

BMC Complement Med Ther

View full text Add to dashboard Cite

Background: Danhong injection (DHI), which is one of the most well-known Traditional Chinese Medicine (TCM) injections, widely used to treat unstable angina (UA). However, its underlying pharmacological mechanisms need to be further clarified. Methods: In the present study, network pharmacology was adopted. Firstly, the relative compounds were obtained by a wide-scaled literatures-mining and potential targets of these compounds by target fishing were collected. Then, we built the UA target database by DisGeNET, DigSee, TTD, OMIM. Based on data, protein-protein interaction (PPI) analysis, GO and KEGG pathway enrichment analysis were performed and screen the hub targets by topology. Furthermore, evaluation of the binding potential of key targets and compounds through molecular docking. Results: The results showed that 12 ingredients of DHI and 27 putative known therapeutic targets were picked out. By systematic analysis, identified 4 hub targets (TNF, TLR4, NFKB1 and SERPINE1) mainly involved in the complex treating effects associated with coagulation and hemostasis, cell membrane region, platelet alpha granule, NFkappa B signaling pathway and TNF signaling pathway. Conclusion: The results of this study preliminarily explained the potential targets and signaling pathways of DHI in the treatment of UA, which may help to laid a good foundation for experimental research and further clinical application.

show abstract

Rapid quantitation and identification of the chemical constituents in Danhong Injection by liquid chromatography coupled with orbitrap mass spectrometry

Cited by 27 publications

References 23 publications

Danhong Injection Attenuates Cerebral Ischemia-Reperfusion Injury in Rats Through the Suppression of the Neuroinflammation

Danhong Injection Attenuates Cerebral Ischemia-Reperfusion Injury in Rats Through the Suppression of the Neuroinflammation

Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Investigating the multi-target pharmacological mechanism of danhong injection acting on unstable angina by combined network pharmacology and molecular docking

Contact Info

Product

Resources

About