Rapid quantitation of mixed red cell populations using flow cytometry

Nelson, Margaret; Popp, H; Forsyth, Cecily; Gibson, John

doi:10.1046/j.1365-2257.1996.00165.x

Cited by 10 publications

(6 citation statements)

References 2 publications

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“…Wagner and Flegel (84) also used an indirect method to detect chimerism following bone marrow transplantation. Nelson et al (85) used an indirect method using unlabeled Rh antibodies to sensitize RBCs followed by a biotin-conjugated sheep AHG and a streptavidin-phycoerythin conjugate. This was followed by treatment with thiazole orange to detect reticulocytes, which would detect the RBCs presently being produced by the bone marrow of the recipient.…”

Section: Blood Group Chimerismmentioning

confidence: 99%

Applications of flow cytofluorometry to red blood cell immunology

Garratty

Arndt

1999

Cytometry

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Section: Blood Group Chimerismmentioning

confidence: 99%

Applications of flow cytofluorometry to red blood cell immunology

Garratty

Arndt

1999

Cytometry

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“…The analysis of mixed RBC populations occurring as a result of hematopoietic chimerism in twins [42][43][44][45][46] and in bone marrow transplant recipients [47][48][49][50][51][52] is easily performed by flow cytometry. This technique has also been used in studies of individuals with blood group mosaicism [26,[53][54][55].…”

Section: Chimerism and Mosaicismmentioning

confidence: 99%

“…Fluorescence may be enhanced by the use of a biotinylated secondary antibody followed by avidin or streptavidin conjugated to FITC or PE [71]. In addition, a third antibody directed against the secondary immunoglobulin or fluorochrome can be added for enhancement of weak signals [48,72]. Antibodies that have been used to label blood group antigens for analysis of mixed RBC populations are listed in Table II.…”

Section: Secondary Antibodiesmentioning

confidence: 99%

Blood doping in athletes—Detection of allogeneic blood transfusions by flow cytofluorometry

Arndt

Kumpel

2008

American J Hematol

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Athletes may undergo blood transfusion to increase their red cell mass and the oxygen carrying capacity of their blood in order to confer a competitive advantage. Allogeneic transfusions are normally mismatched at one or more minor blood group antigens. The most sensitive and accurate method known to detect this form of blood doping is flow cytometry. Low percentages of antigen-positive and antigen-negative red blood cells (RBCs) can be quantitated using suitable specific alloantibodies and careful analysis. By testing blood samples taken at various times, a reduction in the percentage of a minor population of RBCs will indicate transfusion has occurred. Am. J. Hematol. 83:657-667, 2008. V

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“…FC has been found to be useful when studying the mixed RBC populations found in twin chimeras [118][119][120][121][122] and for studying mosaicism seen in individuals with Tn syndrome [123], in female carriers of the X-linked McLeod gene [119,124,125] and in ABO [99] and Rh mosaics [48]. RBC chimerism that occurs after bone marrow transplantation (BMT) has also been studied by FC [126][127][128][129][130][131]. This type of study requires that there not only be an antigen difference between the bone marrow donor and the recipient but also that transfused units, both pre-and post-transplant, be carefully chosen based on antigen type.…”

Section: Hematopoietic Chimerism and Mosaicismmentioning

confidence: 99%

Flow Cytofluorometric Analysis in Red Blood Cell Immunology

Arndt¹,

Garratty²

2004

Transfus Med Hemother

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Flow cytofluorometry (FC) is primarily used in the clinical laboratory for the analysis of white blood cells but also has applications in the study of red blood cell (RBC) immunology. When studying RBCs by FC it is important to avoid agglutination of the RBCs, which ironically is the endpoint for most immunohematologic assays. When studying low levels of RBC-bound antibody or RBC antigens with low copy number, background noise due to autofluorescence and nonspecific binding need to be taken into account. It is also important to ensure that reagents (e.g. antisera) used by FC have been standardized for use by FC. Applications of FC in RBC immunology include i) detection and quantitation of globulin (RBC-bound or free in serum), ii) detection and quantitation of RBC antigens, and iii) detection and quantitation of mixed cell populations. FC can be used to differentiate the amount of IgG on strongly IgG-coated RBCs, as well as to detect small amounts of RBC-bound IgG. FC has been used to quantitate antibody that is free in serum, e.g. anti-D in a pregnant woman or in immune globulin preparations. Many RBC antigens have been studied by FC to determine the number of antigen sites, the density distribution, and phenotype-related differences (e.g. zygosity). FC has been found to be very useful for studying mixed cell populations; applications include fetomaternal hemorrhage quantitation, determination of survival/ clearance of transfused RBCs, phenotyping of transfused patients’ RBCs, evaluation of bone marrow transplant engraftment, and study of RBC chimerism and mosaicism.

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Rapid quantitation of mixed red cell populations using flow cytometry

Cited by 10 publications

References 2 publications

Applications of flow cytofluorometry to red blood cell immunology

Applications of flow cytofluorometry to red blood cell immunology

Blood doping in athletes—Detection of allogeneic blood transfusions by flow cytofluorometry

Flow Cytofluorometric Analysis in Red Blood Cell Immunology

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