Rapid quantitation of three synthetic cathinones in urine by magnetic dispersive solid-phase extraction combined with DART-HRMS

Abstract

For the rapid quantitation of three synthetic cathinones, including 1-(4-Chlorophenyl)-2-(1-pyrrolidinyl)pentan-1-one (4-Cl-α-PVP), 1-(4-Methylphenyl)-2-(methylamino)pentan-1-one (4-MPD), and 1-(5,6,7,8-Tetrahydronaphthalen-2-yl)-2-(1-pyrrolidinyl)pentan-1-one (β-TH-Naphyrone), in urine, a new method was established using magnetic dispersive solid-phase extraction (MDSPE) combined...

Automatic MDSPE Combined with DART–HRMS for the Rapid Quantitation of 21 Synthetic Cathinones in Urine

“…Three matrices from different sources were adjusted by pH 7 buffer, and their pH could be adjusted to the range of pH 6.73 ± 0.04, indicating the strong adjustment ability of the buffer. The previous experiment 27 showed that stable ionization conditions could not be achieved when ethyl acetate was used as the eluent, and the elution ability of methanol and ethanol was not as good as that of acetonitrile for synthetic cathinones in MDSPE. Therefore, the effects of acetonitrile and 0.1% formic acid-acetonitrile as eluents on the analytes were investigated in this experiment.…”

“…MDSPE can also be equipped with an automatic extractor to process batches of samples, which makes the operation simpler, labor-saving, and more efficient. However, no studies have reported MDSPE-based methods for the determination of synthetic cathinones except our previous report …”

“…However, no studies have reported MDSPE-based methods for the determination of synthetic cathinones except our previous report. 27 The purpose of this research was to establish a new method for the rapid quantification of 21 synthetic cathinones in urine by automatic MDSPE combined with DART−HRMS and to strive to obtain the quantitative results of test materials accurately in a very short time. In addition, the quantitative method for analyzing synthetic cathinones in urine by MDSPE combined with LC−MS/MS was planned to establish for comparison with the method of MDSPE combined with DART−HRMS to verify the accuracy of this method in many aspects.…”

“…Direct analysis in real time (DART), a versatile ion source for the analysis of materials in open air under ambient conditions, is proposed by Cody in 2005 . The results could be obtained quickly within 1 min by DART–MS without chromatographic separation, , which was very advantageous for quantification. − And, aside from being cumbersome and time-consuming, the major limitation of sample preparation is that sample extraction sometimes requires solvents that are not compatible with LC/GC systems. The advantage of DART is that the sample is not required to be evaporated unless it is required to be concentrated.…”

Simultaneous Determination of 16 Kinds of Synthetic Cathinones in Human Urine Using a Magnetic Nanoparticle Solid-Phase Extraction Combined with Gas Chromatography-Mass Spectrometry

“…This process is time saving, low cost and environmentally friendly [16]. MSPE has been widely applied in environmental pollutant analysis [17] and drug analysis [18], especially magnetic carbon nanoparticles as the adsorbent [19]. However, there are few reports on the application of MSPE in the determination of synthetic cathinones, especially in biological matrix.…”