Rapid quantitative determination of oleuropein in olive leaves (Olea europaea) using mid-infrared spectroscopy combined with chemometric analyses

Aouidi, Fathia; Dupuy, Nathalie; Artaud, Jacques; Roussos, Sévastianos; Msallem, M.; Gaime, Isabelle Perraud; Hamdi, Moktar

doi:10.1016/j.indcrop.2011.12.024

Cited by 64 publications

(39 citation statements)

References 30 publications

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“…The method validation was performed in accordance to the International Conference on Harmonisation (ICH) Q2 (R1) guidelines by evaluating stability, specificity, linearity, precision, accuracy, quantitation and detection limit, robustness and system suitability parameters [30]. …”

Section: Methodsmentioning

confidence: 99%

“…Thus, OE has been analysed employing HPLC-UV [25], HPLC-fluorescence [26] and HPLC-MS/MS [23,27]. Other methods besides liquid chromatography have also been employed such as GC-MS [28], capillary electrophoresis [29] and FT-IR [30]. However no analytical methodologies have been developed and validated for the simultaneous quantification of OE and its main degradation product HT in HMP’s.…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation of a Combined Methodology for Assessing the Total Quality Control of Herbal Medicinal Products – Application to Oleuropein Preparations

et al. 2013

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Oleuropein (OE) is a secoiridoid glycoside, which occurs mostly in the Oleaceae family presenting several pharmacological properties, including antioxidant, cardio-protective, anti-atherogenic effects etc. Based on these findings OE is commercially available, as Herbal Medicinal Product (HMP), claimed for its antioxidant effects. As there are general provisions of the medicine regulating bodies e.g. European Medicines Agency, the quality of the HMP’s must always be demonstrated. Therefore, a novel LC-MS methodology was developed and validated for the simultaneous quantification of OE and its main degradation product, hydroxytyrosol (HT), for the relevant OE claimed HMP’s. The internal standard (IS) methodology was employed and separation of OE, HT and IS was achieved on a C18 Fused Core column with 3.1 min overall run time employing the SIM method for the analytical signal acquisition. The method was validated according to the International Conference on Harmonisation requirements and the results show adequate linearity (r2 > 0.99) over a wide concentration range [0.1–15 μg/mL (n=12)] and a LLOQ value of 0.1 μg/mL, for both OE and HT. Furthermore, as it would be beneficial to control the quality taking into account all the substances of the OE claimed HMP’s; a metabolomics-like approach has been developed and applied for the total quality control of the different preparations employing UHPLC-HRMS-multivariate analysis (MVA). Four OE-claimed commercial HMP’s have been randomly selected and MVA similarity-based measurements were performed. The results showed that the examined samples could also be differentiated as evidenced according to their scores plot. Batch to batch reproducibility between the samples of the same brand has also been determined and found to be acceptable. Overall, the developed combined methodology has been found to be an efficient tool for the monitoring of the HMP’s total quality. Only one OE HMP has been found to be consistent to its label claim.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Combined Methodology for Assessing the Total Quality Control of Herbal Medicinal Products – Application to Oleuropein Preparations

et al. 2013

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“…The bands at 1710, 1620, and 1530 cm −1 corresponded to C=O and C=C stretching vibrations [35], one of which at 1710 cm −1 strengthened and shifted to high wavenumber, and the other bands shifted after being adsorbed. The endocyclic and exocyclic C-O stretching vibrations of carbohydrates (1080, 1040, and 920 cm −1 ) could be observed in the spectrum of oleuropein [35]. The bands at 1066, 1026, and 991 cm −1 were due to deformation vibration of C-H and stretching vibration of C-O of C-CNT, shifting and overlapping with the bands of C-O stretching vibration of oleuropein after its adsorption by the porous material.…”

Section: Adsorption Mechanismmentioning

confidence: 99%

“…A broad band at about 3400 cm −1 was ascribed to stretching vibration of -OH in oleuropein [35], and the band shifted after being adsorbed. The bands at 1710, 1620, and 1530 cm −1 corresponded to C=O and C=C stretching vibrations [35], one of which at 1710 cm −1 strengthened and shifted to high wavenumber, and the other bands shifted after being adsorbed. The endocyclic and exocyclic C-O stretching vibrations of carbohydrates (1080, 1040, and 920 cm −1 ) could be observed in the spectrum of oleuropein [35].…”

Section: Adsorption Mechanismmentioning

confidence: 99%

Adsorption behaviors for oleuropein from olive leaves extracts by porous materials with carbon nanotubes

Liu

Zhang

et al. 2015

Colloid Polym Sci

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Porous materials with carboxyl-modified multiwalled carbon nanotube (C-CNT) were prepared and applied to enrich oleuropein. Surface area, pore volume, and average pore diameter increased with increasing C-CNT content, and adsorption capacity was greatly enhanced. The results indicated that pseudo-second-order kinetics could describe the whole adsorption process, while intraparticle diffusion model fit the experimental data for the second stage (10-300 min). It suggested Freundlich isotherm model could describe the adsorption process. It showed that the porous materials exhibited excellent reusability and during six adsorption-desorption cycles, adsorption capacities showed no obvious loss. The high adsorption capacities, easy desorption, and remarkable reusability made the prepared porous materials as novel adsorbents for the enrichment of oleuropein. This study indicates porous materials with carboxyl group, large surface area, pore volume, and average pore diameter are favorable for oleuropein enrichment, providing an idea to design porous materials with carboxyl groups for oleuropein enrichment.

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“…The application of chemometric is increasing as a need to cover more areas of research (Pomerantsev and Rodionova 2010). The chemometric analyses have been used in food investigations, plant extracts and clinical chemistry (Howells et al 1992;Defernez et al 1995;Vogels et al 1996;Ellis et al 2007;Trygg et al 2007;Lai et al 2010;Aouidi et al 2012). Up to now, a protocol of sample preparations for MALDI analysis of lichen proteins is not reported, as well as no content information about the type of proteins present in lichen species is available.…”

Section: Introductionmentioning

confidence: 99%

Chemotaxonomic Fingerprinting of Chilean Lichens Through Maldi and Electrospray Ionization Mass Spectrometry

Santos

Soriano

Mirabal‐Gallardo

et al. 2015

Braz. arch. biol. technol.

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The aim of this work was to study a fast, new, sensitive, and simple method for the chemotaxonomic classification of Chilean lichens (Teloschistes chrysophthalmus, Ramalina farinacea, Usnea pusilla, Ramalina chilensis and Stereocaulon ramulosum) using MALDI-TOF-MS and UPLC-ESI(-)-MS data. Lichens soluble proteins fingerprints were acquired by MALDI-TOF-MS and they were analyzed by chemometric (PCA). Lichens organic extracts fingerprints were obtained by UPLC-ESI(-)-MS. MALDI-TOF-MS associated with chemometric analysis was used to detect new m/z patterns of soluble proteins that were compared with Protein Data Bank of UnitPro. These data also permitted the satisfactory distinction among the families and species. UPLC-ESI(-)-MS fingerprints analyses of the organic extracts showed the presence of five major lichen compounds (atranorin, parietin, teloschistin, ramalinolic and usnic acids). In contrast to other techniques, MALDI-TOF-MS associated with chemometric analysis and UPLC-ESI(-)-MS provided a new, fast and sensitive method for chemotaxonomic characterization of lichens.

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Rapid quantitative determination of oleuropein in olive leaves (Olea europaea) using mid-infrared spectroscopy combined with chemometric analyses

Cited by 64 publications

References 30 publications

Development and Validation of a Combined Methodology for Assessing the Total Quality Control of Herbal Medicinal Products – Application to Oleuropein Preparations

Development and Validation of a Combined Methodology for Assessing the Total Quality Control of Herbal Medicinal Products – Application to Oleuropein Preparations

Adsorption behaviors for oleuropein from olive leaves extracts by porous materials with carbon nanotubes

Chemotaxonomic Fingerprinting of Chilean Lichens Through Maldi and Electrospray Ionization Mass Spectrometry

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