Rapid Quantitative Method for Salmonella Detection in Polluted Waters

Cheng, Chu Ming; Boyle, William C.; Goepfert, J. M.

doi:10.1128/aem.21.4.662-667.1971

Cited by 8 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salmonella concentrations in wastewater average from a few to 8000 organisms/100 mL (Feachem et al, 1983). Salmonella concentrations can be 3 to 6 orders of magnitude lower than those of the coliform bacteria, making quantification of Salmonella difficult (Cheng et al, 1971). For this reason, experimental treatment systems were seeded with batch cultures of Salmonella choleraesuis (serotype typhimurium) to determine their removal efficiencies.…”

Section: Introductionmentioning

confidence: 99%

Seasonal Pathogen Removal by Alternative On‐Site Wastewater Treatment Systems

Pundsack¹,

Axler²,

Hicks³

et al. 2001

Water Environment Research

View full text Add to dashboard Cite

Subsurface-flow constructed wetlands, sand filters, and peat filters near Duluth, Minnesota, were studied to determine their seasonal performance for removing pathogens from wastewater. Influent was a high-strength septic tank effluent (mean values of 5-day biochemical oxygen demand, total nitrogen, and total phosphorus were 294, 96, and 15 mg/L, respectively) at the Natural Resources Research Institute's alternative treatment system test facility in northern Minnesota. Each treatment system was inoculated with cultures of Salmonella choleraesuis (serotype typhimurium) for 5 to 7 consecutive days in summer and winter during 1998 to 1999. After the seeding, outflow samples were taken until Salmonella counts were sustained at background levels. The removal of Salmonella was calculated for each system, although the exact removal mechanisms were not determined. During the summer, the wetlands removed 99.6 to 99.999 4% (2.4 to 5.3 log 10 reduction) of the culturable Salmonella. The sand filters demonstrated a greater than 7 log 10 removal of Salmonella cells, whereas the peat filters were responsible for a greater than 8 log 10 loss of cells. Fewer Salmonella cells were removed by all of these systems during the winter, although the pattern of removal was similar to their summer operation. During the winter, the wetlands and sand filters removed greater than 1 log 10 of culturable cells, but the peat filters were responsible for a greater than 5 log 10 loss of cells. Fecal coliform removal patterns reflected those for Salmonella by treatment systems for summer and winter periods. Based on Salmonella and fecal coliform removal, the peat filters operated most effectively followed by the sand filters and the constructed wetlands. Water Environ. Res., 73, 204 (2001).

show abstract

Section: Introductionmentioning

confidence: 99%

Seasonal Pathogen Removal by Alternative On‐Site Wastewater Treatment Systems

Pundsack¹,

Axler²,

Hicks³

et al. 2001

Water Environment Research

View full text Add to dashboard Cite

show abstract

“…Enteric bacteria in sludge. The procedure we used for the isolation of salmonellae from sludge is similar to that described by Dudley et al (9), in that we too used Selenite broth as one of our enrichment media and incubated the enrichment at 37°C rather than at 42°C as suggested by various authors (4,14,20,21). Edgar and Soar (10), in a study comparing methods for the isolation of salmonellae, reported that several methods described in the literature produced different results not only because some of the media used were inhibitory for the organisms but also because, in some instances, some were inhibitory when used at higher incubation temperatures.…”

Section: Discussionmentioning

confidence: 99%

Multiyear study of sludge application to farmland: prevalence of bacterial enteric pathogens and antibody status of farm families

Ottolenghi¹,

Hamparian²

1987

Appl Environ Microbiol

View full text Add to dashboard Cite

We describe our experience with the isolation of salmonellae from sewage sludge from four treatment plants in different geographic areas of Ohio. Over 3 years, we isolated salmonellae 50 times from 311 sludge samples. Most isolations were made after enrichment in Selenite broth (BBL Microbiology Systems, Cockeysville, Md.). The largest proportion of isolations came from the plant serving the population of Columbus, a large metropolitan area. A significantly greater number of isolations from this plant were made during the first quarter of the year. Twenty-one different serotypes were isolated, along with five untypable strains. The most frequently isolated serotype was Salmonella infantis. Five of the strains were multiply resistant to antibiotics. We also describe the prevalence of antibodies to salmonellae in members of the families residing on the farms in the study. It was found that antibodies to group C salmonellae predominated.

show abstract

Influence of temperature and time of incubation on the estimation of bacterial numbers in tropical surface waters

Jen

Bell

1982

Water Research

View full text Add to dashboard Cite

Rapid Quantitative Method for Salmonella Detection in Polluted Waters

Cited by 8 publications

References 4 publications

Seasonal Pathogen Removal by Alternative On‐Site Wastewater Treatment Systems

Seasonal Pathogen Removal by Alternative On‐Site Wastewater Treatment Systems

Multiyear study of sludge application to farmland: prevalence of bacterial enteric pathogens and antibody status of farm families

Influence of temperature and time of incubation on the estimation of bacterial numbers in tropical surface waters

Contact Info

Product

Resources

About