Rapid recovery of leydig cell population in rar cryptorchid testes after ethanedime-thanesulfonate injury: Immuno-histochemical studies.

Abstract

Precursor cells in the rapid recovery of Leydig cells after depletion by ethanedimethanesulfonate (EDS) injury to bilateral abdominal testes were examined immunohistochemically 8, 11, and 14 days after an intraperitoneal injection of EDS, 75 mg/kg, 2 weeks after cryptorchidization.Compared with shamoperated rats, the repopulation of Leydig cells in cryptorchid rats was accelerated. Row-like arranged fusiform-shaped mesenchymal cells were found in peritubular regions of abdominal testes 14 days after EDS injection. At 11 days after injection, a significant increase of bromodeoxyuridine-incorporated nuclei was noted in peritubular mesenchymal cells of abdominal testes when compared with scrotal testes. In intertubular tissues, both attenuated mesenchymal cells in earlier stages and fusiform-shaped mesenchymal cells in later stages were actin-, desmin-, and vimentin-negative, and the cells later revealed marked pyronophilia.Therefore, division of peritubular mesenchymal cells seems to contribute somewhat to the rapid recovery of abdominal testes, although differentiation through cytoplasmic pyronophilia from a pool of mesenchymal stem cells give rise to the main part of new Leydig cell populations.

Myoid cell proliferation in rat seminiferous tubules after ischaemic testicular atrophy induced by epinephrine. Morphometric and immunohistochemical (bromo‐deoxyuridine and PCNA) studies*

“…It has been shown that myoid cells in the adult rat proliferate at a low rate (Teerds et al, 1989). However, the myoid cells surrounding damaged seminiferous tubules could undergo proliferation similar to that reported for the Leydig cells in cryptorchid rats subjected to treatment with ethane dimethane sulphonate (Kerr & Donachie, 1986;Sharpe et al, 1990;Naka et al, 1991).…”

“…Both testes were removed and fixed by immersion in methacarn for several minutes. The testicular capsule was then punctured with a thin needle to make small holes to improve infiltration of the fixative and avoid organ deformhtion (Naka et al, 1991). The testes were fixed further in methacarn for 10 h, and then cross-sectioned into 3 mm thick slices which were embedded in paraffin.…”

Immunohistochemical quantitative study of the peritubular lamina propria after induction of testicular atrophy induced by epinephrine

“…Animals were killed by exsanguination under ether anaesthesia. Both testes were removed and fixed by immersion in methacarn for several minutes; the testicular albuginea was then punctured with a thin needle to improve fixative infiltration and avoid organ deformation (Naka et a/., 1991). The testes were further fixed in methacarn for 10 h, and then cross-sectioned into 3-mm thick slices which were embedded in paraffin.…”