Rapid replacement of prevailing genotype of human respiratory syncytial virus by genotype ON1 in Beijing, 2012–2014

Cui, Guanglin; Zhu, Rong; Zhao, Linqing; Sun, Yu; Wang, Fang; Qian, Yuan

doi:10.1016/j.meegid.2015.04.025

Cited by 27 publications

(27 citation statements)

References 29 publications

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“…The second cluster of NA1 study sequences grouped with sequences from India [20]. The ON1 study sequences with 72bp duplication in the G protein gene clustered with sequences from China, Canada, South Africa, Japan, South Korea, Spain, Germany, USA, Italy and India [2,5,15,18,26,69,70,71,72]. This is the first report of ON1 genotype of RSV from Saudi Arabia.…”

Section: Discussionmentioning

confidence: 98%

Co-Circulation of 72bp Duplication Group A and 60bp Duplication Group B Respiratory Syncytial Virus (RSV) Strains in Riyadh, Saudi Arabia during 2014

et al. 2016

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Respiratory syncytial virus (RSV) is an important viral pathogen of acute respiratory tract infection (ARI). Limited data are available on molecular epidemiology of RSV from Saudi Arabia. A total of 130 nasopharyngeal aspirates were collected from children less than 5 years of age with ARI symptoms attending the Emergency Department at King Khalid University Hospital and King Fahad Medical City, Riyadh, Saudi Arabia between October and December, 2014. RSV was identified in the 26% of the hospitalized children by reverse transcriptase PCR. Group A RSV (77%) predominated during the study as compared to group B RSV (23%). The phylogenetic analysis of 28 study strains clustered group A RSV in NA1 and ON1 genotypes and group B viruses in BA (BA9) genotype. Interestingly, 26% of the positive samples clustered in genotypes with duplication in the G protein gene (ON1 for group A and BA for group B). Both the genotypes showed enhanced O-linked glycosylation in the duplicated region, with 10 and 2 additional sites in ON1 and BA respectively. Selection pressure analysis revealed purifying selection in both the ON1 and BA genotypes. One codon each in the ON1 (position 274) and BA genotypes (position 219) were positively selected and had high entropy values indicating variations at these amino acid positions. This is the first report describing the presence of ON1 genotype and the first report on co-circulation of two different genotypes of RSV with duplication in the G protein gene from Saudi Arabia. The clinical implications of the simultaneous occurrence of genotypes with duplication in G protein gene in a given population especially in the concurrent infections should be investigated in future. Further, the ongoing surveillance of RSV in this region will reveal the evolutionary trajectory of these two genotypes with duplication in G protein gene from largest country in the Middle East.

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Section: Discussionmentioning

confidence: 98%

Co-Circulation of 72bp Duplication Group A and 60bp Duplication Group B Respiratory Syncytial Virus (RSV) Strains in Riyadh, Saudi Arabia during 2014

et al. 2016

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“…ON1 has a significantly more rapid evolutionary rate compared with NA1, the previous emerging variant, driven by amino acid substitution in the hypervariable region that is thought to contain the antigenic epitopes for neutralising antibodies . This genotype has rapidly spread to different countries in only 4 years . Recent publications reported that the sequences of recently circulating ON1 strains were different when compared with the original ON1 strains from Canada, with at least 3 different ON1 lineages circulating in the world in 2014.…”

Section: Rsv Diversity and Clinical Featuresmentioning

confidence: 99%

Molecular epidemiology of respiratory syncytial virus

Pangesti

Ghany

Walsh

et al. 2018

Reviews in Medical Virology

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Respiratory syncytial virus (RSV) is a major cause of viral acute respiratory tract infections in young children. The virus is characterised by distinct seasonality that is dependent upon the latitude and its ability to cause reinfection. Respiratory syncytial virus demonstrates a complex molecular epidemiology pattern as multiple strains and/or genotypes cocirculate during a single epidemic. Previous studies have investigated the relationship between RSV genetic diversity, reinfection, and clinical features. Here, we review the evidence behind this relationship together with the impact that the advancement of whole genome sequencing will have upon our understanding and the need for reconsidering the classification of RSV genotypes.

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“…To date, 14 RSV-A subtypes (GA1-7, SAA1-2, NA1-4, and ON1 [Peret et al, 1998[Peret et al, , 2000Venter et al, 2001;Shobugawa et al, 2009;Eshaghi et al, 2012;Pretorius et al, 2012;Ren et al, 2014]) and 22 RSV-B subtypes (GB1-5, SAB1-4, URU1-2, BA1-10, and THB [Peret et al, 1998[Peret et al, , 2000Venter et al, 2002;Dapat et al, 2010;Trento et al, 2010;Auksornkitti et al, 2014;Ren et al, 2015]) have been described. The newly identified ON1 subtype of RSV-A, which was first reported in Canada in 2010 [Eshaghi et al, 2012], contains a unique 72-nucleotide duplication in the second hypervariable region of the G gene and may be spreading rapidly, perhaps replacing NA1, another prevalent RSV-A subtype, in countries such as China, South Korea, Germany, and Italy [Kim et al, 2014;Pierangeli et al, 2014;Tabatabai et al, 2014;Cui et al, 2015]. A similar duplication phenomenon in the variable region of the G protein was also observed in the BA subtype of RSV-B in 1999 in Buenos Aries, Argentina [Trento et al, 2003]; this strain spread rapidly to additional countries, resulting in the current prevalence of RSV-B infections [Dapat et al, 2010;Trento et al, 2010].…”

Section: Introductionmentioning

confidence: 99%

Respiratory syncytial virus subtype ON1/NA1/BA9 predominates in hospitalized children with lower respiratory tract infections

Fan

Zhang

et al. 2016

Journal of Medical Virology

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Respiratory syncytial virus (RSV) infection is the leading cause of acute respiratory tract disease in children less than 5 years old. The aim of this study was to further elucidate the molecular properties and clinical characteristics of RSV infection. The study sample included 238 patients <5 years old who were hospitalized with clinical symptoms of upper or lower respiratory tract infection (URTI or LRTI) in the Pediatric Department at the First People's Hospital of Chenzhou, South China in 2014. We subjected nasopharyngeal aspirate (NPA) or nasal swab (NS) samples from the patients to indirect fluorescence assay screens. RSV G genes were amplified by reverse transcription-PCR (RT-PCR) and sequenced. Of the 238 patients screened, 64 (26.8%) were confirmed to have RSV infections. Of those 64 confirmed RSV infection cases, 39 (60.9%) had subtype BA9, 13 (20.3%) had the recently identified subtype ON1, 11 (17.2%) had subtype NA1, and 1 (1.6%) had subtype GB2. The predominant presentation was LRTI with coughing, sputum production, fever, and wheezing. RSV subtype NA1 and BA9 infections were found mostly in infants, whereas the age distribution of subtype ON1 infections was more uniform across the age bands. Phylogenetic analysis indicated that, compared with the prototype strain A2, all ON1 and most NA1 isolates had lost one potential N-glycosylation site at amino acid 251 and 249 due to T251K and N249Y substitution, respectively. These findings suggest that NA1, BA9, and ON1 are the dominant RSV subtypes causing respiratory tract infections in young children presenting to the hospital in South China. J. Med. Virol. 89:213-221, 2017. © 2016 Wiley Periodicals, Inc.

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Rapid replacement of prevailing genotype of human respiratory syncytial virus by genotype ON1 in Beijing, 2012–2014

Cited by 27 publications

References 29 publications

Co-Circulation of 72bp Duplication Group A and 60bp Duplication Group B Respiratory Syncytial Virus (RSV) Strains in Riyadh, Saudi Arabia during 2014

Co-Circulation of 72bp Duplication Group A and 60bp Duplication Group B Respiratory Syncytial Virus (RSV) Strains in Riyadh, Saudi Arabia during 2014

Molecular epidemiology of respiratory syncytial virus

Respiratory syncytial virus subtype ON1/NA1/BA9 predominates in hospitalized children with lower respiratory tract infections

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