Rapid resolution liquid chromatography method for determination of malathion in pesticide formulation

Abstract

This paper presents a new, simple, precise, and accurate rapid resolution liquid chromatography (RRLC) method for determination of an active ingredient malathion in the pesticide formulation. The analysis was performed on a Poroshell EC 120-C18 (50 mm × 3 mm, 2.7 μm) analytical column using isocratic elution with a mobile phase consisted of acetonitrile–water (50:50, v/v), a flow rate of 1 mL/min, a constant column temperature at 25 °C, and ultraviolet-diode array detection (UV-DAD) at 220 nm. The specificity, selectivity, linearity, precision, and accuracy were tested for the method validation according to the Collaborative International Pesticides Analytical Council (CIPAC) and Directorate General Health and Consumer Protection (SANCO) guidelines and all tested parameters were found within acceptance criteria. The obtained results indicated that the proposed method can be used for routine analysis of the active ingredient malathion in the pesticide formulation “Etiol tecni” following the CIPAC and SANCO rules. The run time of analysis under the stipulated chromatographic conditions was about 2.5 min, meaning that the proposed method requires a small volume (< 1.5 mL) of the organic solvent (acetonitrile) making it cost-effective.

Resistensi Insektisida Malation, DDT, Piretreoid, dan Cypermetrin terhadap Nyamuk A.E Aegypti dan A.E Albopictus: Study Literature

“…Insektisida organ ofosfat banyak digunakan karena dekomposisi yang relatif cepat dan akumulasi yang rendah dalam kehidupan organisme. (Velkoska-Markovska and Petanovska-Ilievska, 2020) Penggunaan piretroid merupakan bagian integral dari strategi pengendalian hama serangga global, terhitung 15% dari pangsa pasar di seluruh dunia. (Sparks et al, 2020) Insektisida piretroid menargetkan saluran natrium berpintu tegangan (VGSC) di neuron serangga dan mengubah potensial aksi, sehingga mengakibatkan kelumpuhan dan kematian nyamuk.…”

Determination of malathion and its residues by normal-phase high-performance liquid chromatography method

“…The reference methods for the determination of active substance malathion in different pesticide formulations using gas-liquid chromatography (GLC) [20] and gas chromatography (GC) [21] have been published by CIPAC (Collaborative International Pesticides Analytical Council). In the previous work, reversed-phase Rapid Resolution Liquid Chromatography (RP-RRLC) method with ultraviolet diode-array detection (UV-DAD) for the determination of malathion in pesticide formulation has been developed and validated [22].…”