Rapid RP-HPLC Method for the Quantification of Glabridin in Crude Drug and in Polyherbal Formulation

Abstract

A simple, economic, selective, precise and robust method has been developed and validated for the analysis of glabridin in crude drugs and polyherbal formulations. Reversed-phase chromatography is performed on a C18 column with water and acetonitrile as mobile phase in gradient elution method at a flow rate of 1 mL/min. Detection is performed at 230 nm and a sharp peak is obtained for glabridin at a retention time of 14.9 + + + + + 0.02 min. Linear regression analysis data for the calibration plot showed a good linear relationship between response and concentration in the range of 1-500 mg/mL; the regression coefficient is 0.9992 and the linear regression equation is y 5 26.683x-142.17. The method is validated for accuracy, precision, reproducibility, robustness and detection and quantification limits, in accordance with International Conference on Harmonization guidelines. Statistical analysis proved that the method is precise, reproducible, selective and accurate for the analysis of glabridin. The proposed, developed and validated high-performance liquid chromatography method for the quantification of glabridin can be used for the quality control and standardization of licorice (Glycyrrhiza glabra Linn.) and different herbal formulations in which licorice is present as a constituent.

Simultaneous Detection of Glabridin, (−)-α-Bisabolol, and Ascorbyl Tetraisopalmitate in Whitening Cosmetic Creams Using HPLC-PAD

“…The LOD values of 0.03 μg mL −1 (glabridin), 0.4 μg mL −1 ((−)-α-bisabolol), and 4.02 μg mL −1 (ascorbyl tetraisopalmitate) were satisfactory for analysis. The current LODs were considerably lower than those reported for ascorbyl tetraisopalmitate in cosmetic products (15.05 μg mL −1 [15]), glabridin in crude drug (0.35 μg mL −1 [25]), and (−)-α-bisabolol in particulate systems (0.5 μg mL −1 [28]). Recoveries at three fortification levels were ranged from 89.8 to 103.9 % with RSD < 5 % ( Table 4).…”

“…In this context, Almeida et al used isocratic elution with methanol and isopropyl alcohol (25:75, v/v) to separate tocopheryl acetate and ascorbyl tetraisopalmitate in cosmetic formulations using HPLC [25]. Kamal et al used acetonitrile and deionized water in gradient elution method [15]. In this study, the mobile phase gradient condition described in "Analytical method" was selected to separate the three compounds, as shown in Fig.…”

“…Glabridin has been associated with numerous biological properties such as anticancer, antioxidant, anti-inflammatory, antibacterial, and skin-whitening activities [13][14][15]. Because it is a potent tyrosinase inhibitor, the skin-whitening effect was due to the inhibition of melanin [16].…”

“…Meanwhile, there were a very few literatures for determination of (−)-α-bisabolol in human blood and chamomile flowers, but not cosmetics [29][30][31]. The HPLC method for quantitation of glabridin in polyherbal preparations and crude extracts was evaluated by Kamal et al [15]. Similar to (−)-α-bisabolol, there was no report for detection glabridin in cosmetics, however, it was detected in crude drugs and human plasma [15,32].…”

“…The HPLC method for quantitation of glabridin in polyherbal preparations and crude extracts was evaluated by Kamal et al [15]. Similar to (−)-α-bisabolol, there was no report for detection glabridin in cosmetics, however, it was detected in crude drugs and human plasma [15,32]. So far, no such method is described in the literature neither for simultaneous determination of the three lipophilic compounds (glabridin, (−)-α-bisabolol, and ascorbyl tetraisopalmitate) nor in whitening cosmetic creams.…”