Rapid Salmonella Detection in Foods by Motility Enrichment on a Modified Semi-Solid Rappaport-Vassiliadis Medium

Abstract

Modification of Rappaport-Vassiliadis enrichment broth into a semisolid motility medium (MSRV) provided a sensitive means for detecting Salmonella in contaminated foods. The type of peptone, the concentration of magnesium chloride, the presence of novobiocin and the temperature of incubation were determinants in medium performance. The analytical procedure consists of preenrichment for 20 h, followed by motility enrichment on MSRV for 24 h and, if there is migration, serological tests with the motile culture. The test result is obtained within 48 h from the start of preenrichment. This approach gave 39% more Salmonella-positive samples than enrichment in tetrathionate brilliant green broth with subsequent plating.

Characterization of Salmonella isolates from beef cattle, broiler chickens and human sources on Prince Edward Island

“…al (21) and Desmedt et. al (70). It involved a two step isolation procedure, selective en richment and selective plating.…”

Comparision of the BAX® System with an in-house MSRV method for the detection of Salmonella in chicken carcasses and pork meat

“…This fact is used by several authors (De Smedt et al, 1986) and in the AOAC Official Method 993.07 (AOAC, 1995) to attempt direct serological testing of migrated MSRV cultures, considering them nearly pure cultures (28).…”

“…In the case of development of migration zones equal to or larger than 10 mm, the presence of Salmonella can be expected (12,42).…”

“…In addition, motility-based immunodiffusion tests (modified 1-2 test (35,44) and detection methods based on motility on liquid or semisolid media (6,12,15,23,39) have been used.…”

Purification and Some Properties of a Novel Ethanol Dehydrogenase with High Activity against Dulcitol 1-Phosphate from Salmonella Typhimurium IFO 12529.

“…Rapid detection of Salmonella in foods is important for quality assurance in the food industry. Many methods, such as cultural methods, 2) enzyme immunoassay,3) and DNA-DNA hybridization, 4'5) are available for detecting Estimation of molecular weight by gel filtration. Molecular weight of the purified D1P-EDHwas estimated with;a Shimadzu liquid chromatograph LC-9A with a du Pent Zorbax GF-250 column (9.4 x 250mm).…”