Rapid screening of amitraz and its metabolite residues in honey using a quick, easy, cheap, effective, rugged, and safe extraction method coupled with UHPLC and Q Exactive

Abstract

A method for determining amitraz and 2,4-dimethylaniline in honey was established by using ultra-high-performance liquid chromatoghaphy and Q Exactive after applying quick, easy, cheap, effective, rugged, and safe extracting process. A suitable extraction method was designed to extract the amitraz and 2,4-dimethylaniline after a suitable amount of honey samples was dissolved. A Thermo Syncronis C 18 column (100 × 2.1 mm, 1.7 μm) was used for chromatographic separation of the samples.Then the two compounds were quantitatively analyzed via a program of Q Exactive. The linearity of amitraz and 2,4-dimethylaniline was good in the concentration range of 0.5-100 μg/L, and the correlation coefficient R 2 was >0.99. The average recovery and relative standard deviation of each component were 81.3-90.0% and 5.1-7.2%. The 24-and 48-h test results showed that the sample needed to be tested within 24 h.The limit of detection was 0.1 μg/kg for amitraz and 2,4-dimethylaniline, whereas for both the limit of quantitation was 0.3 μg/kg. K E Y W O R D Samitraz, 2,4-dimethylaniline, honey, orbital trap 1466

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