Rapid screening of aquatic toxicity of several metal-based nanoparticles using the MetPLATE™ bioassay

Pokhrel, Lok R.; Silva, Thilini; Dubey, Brajesh; Badawy, Amro M. El; Tolaymat, Thabet; Scheuerman, Phillip R.

doi:10.1016/j.scitotenv.2012.03.049

Cited by 41 publications

(47 citation statements)

References 49 publications

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“…Different modes of action are supported by other experiments in our laboratory and in other species, where exposure to nTiO 2 and nCeO 2 altered different genes in Arabidopsis thaliana [13]. Similarly, a recent study using 5 different types of metal-based ENMs also noted differential responses in Escherichia coli [2]. Additional research will be necessary to fully identify how these 2 ENMs differ in altering early plant growth and development.…”

Section: Species and Particle Differencessupporting

confidence: 64%

Germination and early plant development of ten plant species exposed to titanium dioxide and cerium oxide nanoparticles

Andersen

King

Plocher

et al. 2016

Enviro Toxic and Chemistry

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134

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Ten agronomic plant species were exposed to different concentrations of nano-titanium dioxide (nTiO2 ) or nano-cerium oxide (nCeO2 ) (0 μg/mL, 250 μg/mL, 500 μg/mL, and 1000 μg/mL) to examine potential effects on germination and early seedling development. The authors modified a standard test protocol developed for soluble chemicals (OPPTS 850.4200) to determine if such an approach might be useful for screening engineered nanomaterials (ENMs) and whether there were differences in response across a range of commercially important plant species to 2 common metal oxide ENMs. Eight of 10 species responded to nTiO2 , and 5 species responded to nCeO2 . Overall, it appeared that early root growth may be a more sensitive indicator of potential effects from ENM exposure than germination. The observed effects did not always relate to the exposure concentration, indicating that mass-based concentration may not fully explain the developmental effects of these 2 ENMs. The results suggest that nTiO2 and nCeO2 have different effects on early plant growth of agronomic species, with unknown effects at later stages of the life cycle. In addition, standard germination tests, which are commonly used for toxicity screening of new materials, may not detect the subtle but potentially more important changes associated with early growth and development in terrestrial plants. Environ Toxicol Chem 2016;35:2223-2229. Published 2016 Wiley Periodicals Inc. on behalf of SETAC. This article is a US Government work and, as such, is in the public domain in the United States of America.

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Section: Species and Particle Differencessupporting

confidence: 64%

Germination and early plant development of ten plant species exposed to titanium dioxide and cerium oxide nanoparticles

Andersen

King

Plocher

et al. 2016

Enviro Toxic and Chemistry

Self Cite

134

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“…01813) and air‐dried before they were imaged using a Philips EM420 microscope operating at 120 kV in brightfield mode . Mean physical diameter and particle size distribution were determined from representative images using ImageJ 1.44 software . Mean physical particle sizes in stock suspensions and diluted suspensions matched (nano‐titania) or were smaller than (nano‐ceria) values indicated by the manufacturer (Figure ).…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and genomic responses to titanium dioxide and cerium oxide nanoparticles in Arabidopsis germinants

Tumburu

Andersen

Rygiewicz

et al. 2014

Enviro Toxic and Chemistry

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The effects of exposure to nanoparticles of titanium dioxide (nano-titanium) and cerium oxide (nano-cerium) on gene expression and growth in Arabidopsis thaliana germinants were studied by using microarrays and quantitative real-time polymerase chain reaction (qPCR), and by evaluating germinant phenotypic plasticity. Exposure to 12 d of either nano-titania or nano-ceria altered the regulation of 204 and 142 genes, respectively. Genes induced by the nanoparticles mainly include ontology groups annotated as stimuli responsive, including both abiotic (oxidative stress, salt stress, water transport) and biotic (respiratory burst as a defense against pathogens) stimuli. Further analysis of the differentially expressed genes indicates that both nanoparticles affected a range of metabolic processes (deoxyribonucleic acid [DNA] metabolism, hormone metabolism, tetrapyrrole synthesis, and photosynthesis). Individual exposures to the nanoparticles increased percentages of seeds with emergent radicles, early development of hypocotyls and cotyledons, and those with fully grown leaves. Although there were distinct differences between the nanoparticles in their affect on molecular mechanisms attributable to enhancing germinant growth, both particles altered similar suites of genes related to various pathways and processes related to enhanced growth.

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“…Ag + dissolution from Ag 2 SO 4 was greater than that observed for the AgNPs over 51 h (Supporting Information http://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12147/suppinfo) and may account for the observed increase in toxicity compared with AgNPs. Several previous studies have also demonstrated that dissolved silver (usually in the form of AgNO 3 ) is more toxic than AgNPs to bacteria (Choi et al ., ; Jin et al ., ; Pokhrel et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…S3) and may account for the observed increase in toxicity compared with AgNPs. Several previous studies have also demonstrated that dissolved silver (usually in the form of AgNO3) is more toxic than AgNPs to bacteria (Choi et al, 2008;Jin et al, 2010;Pokhrel et al, 2012).…”

Section: Interactions Between E Coli Agnps and Ag2so4mentioning

confidence: 98%

Effects of engineered silver nanoparticles on the growth and activity of ecologically important microbes

Beddow

Stolpe

Cole

et al. 2014

Environ Microbiol Rep

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SummaryCurrently, little is known about the impact of silver nanoparticles (AgNPs) on ecologically important microorganisms such as ammonia-oxidizing bacteria (AOB). We performed a multi-analytical approach to demonstrate the effects of uncapped nanosilver (uAgNP), capped nanosilver (cAgNP) and Ag2SO4 on the activities of the AOB: Nitrosomonas europaea, Nitrosospira multiformis and Nitrosococcus oceani, and the growth of Escherichia coli and Bacillus subtilis as model bacterial systems in relation to AgNP type and concentration. All Ag treatments caused significant inhibition to the nitrification potential rates (NPRs) of Nitrosomonas europaea (decreased from 34 to < 16.7 μM NH4 + oxidized day ). Escherichia coli-Ag interactions revealed that the percentage of damaged E. coli cells was 45% greater with Ag2SO4, 39% with cAgNPs and 33% with uAgNPs compared with controls. Generally, the inhibitory effect on AOB NPRs and E. coli/B. subtilis growth was in the following order Ag2SO4 > cAgNP > uAgNP. In conclusion, AgNPs (especially cAgNPs) and Ag2SO4 adversely affected AOB activities and thus have the potential to severely impact key microbially driven processes such as nitrification in the environment.

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Rapid screening of aquatic toxicity of several metal-based nanoparticles using the MetPLATE™ bioassay

Cited by 41 publications

References 49 publications

Germination and early plant development of ten plant species exposed to titanium dioxide and cerium oxide nanoparticles

Germination and early plant development of ten plant species exposed to titanium dioxide and cerium oxide nanoparticles

Phenotypic and genomic responses to titanium dioxide and cerium oxide nanoparticles in Arabidopsis germinants

Effects of engineered silver nanoparticles on the growth and activity of ecologically important microbes

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