Rapid Screening of Lipase Inhibitors from Ophiopogonis Radix Using High-Performance Thin Layer Chromatography by Two Step Gradient Elution Combined with Bioautographic Method

Xue, Hua; Hong, Huijie; Zhang, Daiyan; Qiao, Liu; Leong, Fong; Yang, Qi; Y, Hu; Chen, Xiao‐Jia

doi:10.3390/molecules27041155

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…The authors of [ 56 ] elaborated a thorough report on usage of the TLC-DB test to screening the Chinese medicine Ophiopogonis Radix (which is the dried root of Ophiopogon japonicus (L.f) Ker-Gawl.) for its ability to inhibit pancreatic lipase.…”

Section: Thin-layer Chromatography Coupled With Bioassaysmentioning

confidence: 99%

Thin-Layer Chromatography (TLC) in the Screening of Botanicals–Its Versatile Potential and Selected Applications

Kowalska

Sajewicz

2022

Molecules

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The aim of this paper is to present a comprehensive overview of the main aims and scopes in screening of botanicals, a task of which thin-layer chromatography (TLC) is, on an everyday basis, confronted with and engaged in. Stunning omnipresence of this modest analytical technique (both in its standard format (TLC) and the high-performance one (HPTLC), either hyphenated or not) for many analysts might at a first glance appear chaotic and random, with an auxiliary rather than leading role in research, and not capable of issuing meaningful final statements. Based on these reflections, our purpose is not to present a general review paper on TLC in screening of botanicals, but a blueprint rather (illustrated with a selection of practical examples), which highlights a sovereign and important role of TLC in accomplishing the following analytical tasks: (i) solving puzzles related to chemotaxonomy of plants, (ii) screening a wide spectrum of biological properties of plants, (iii) providing quality control of herbal medicines and alimentary and cosmetic products of biological origin, and (iv) tracing psychoactive plants under forensic surveillance.

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Section: Thin-layer Chromatography Coupled With Bioassaysmentioning

confidence: 99%

Thin-Layer Chromatography (TLC) in the Screening of Botanicals–Its Versatile Potential and Selected Applications

Kowalska

Sajewicz

2022

Molecules

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“…In paper [59], a thorough report was given on usage of the TLC-DB test to screening the Chinese medicine Ophiopogonis Radix (which is the dried root of Ophiopogon japonicus (L.f) Ker-Gawl.) for its ability to inhibit pancreatic lipase.…”

Section: Thin-layer Chromatography In Screening Of Botanicals For The...mentioning

confidence: 99%

Thin-Layer Chromatography (TLC) in Screening of Botanicals – its Versatile Potential and Selected Applications

Kowalska¹,

Sajewicz²

2022

Preprint

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The aim of this paper is to present a comprehensive overview of the main aims and scopes in screening of botanicals, a task which thin-layer chromatography (TLC) is on an everyday basis confronted with and engaged in. Stunning omnipresence of this modest analytical technique (both in its standard format (TLC) and the high-performance one (HPTLC), either hyphenated or not) for many analysts might at a first glance appear chaotic and random, with an auxiliary rather than leading role in research, and not capable of issuing meaningful final statements. Based on these reflections, our purpose is not to present a general review paper on TLC in screening of botanicals, but a blueprint rather (illustrated with a selection of practical examples), which highlights a sovereign and important role of TLC in accomplishing the following analytical tasks: (i) solving puzzles related to chemotaxonomy of plants, (ii) screening a wide spectrum of biological properties of plants, (iii) providing quality control of herbal medicines and alimentary and cosmetic products of biological origin, and (iv) tracing psychoactive plants under forensic surveillance.

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“…To date, a series of analytical methods including UV-Vis spectrophotometry [ 1 ], near infrared spectroscopy [ 18 ], TLC [ 19 , 20 ], HPLC-UV [ 21 , 22 ], HPLC-ELSD [ 23 , 24 ] and HPLC-MS [ 2 , 4 , 15 , 16 , 17 , 25 ] have been developed for the quality evaluation of MD and/or SMD. Although HPLC-MS has the advantages of high resolution and high sensitivity, the immense cost hinders its wide use in routine laboratories.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Ophiopogon japonicus and Liriope spicata var. prolifera from Different Origins Based on Multi-Component Quantification and Anticancer Activity

et al. 2023

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The tuberous root of Ophiopogon japonicus (Thunb.) Ker-Gawl. is a well-known Chinese medicine also called Maidong (MD) in Chinese. It could be divided into “Chuanmaidong” (CMD) and “Zhemaidong” (ZMD), according to the geographic origins. Meanwhile, the root of Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma (SMD) is occasionally used as a substitute for MD in the market. In this study, a reliable pressurized liquid extraction and HPLC-DAD-ELSD method was developed for the simultaneous determination of nine chemical components, including four steroidal saponins (ophiopojaponin C, ophiopogonin D, liriopesides B and ophiopogonin D’), four homoisoflavonoids (methylophiopogonone A, methylophiopogonone B, methylophiopogonanone A and methylophiopogonanone B) and one sapogenin (ruscogenin) in CMD, ZMD and SMD. The method was validated in terms of linearity, sensitivity, precision, repeatability and accuracy, and then applied to the real samples from different origins. The results indicated that there were significant differences in the contents of the investigated compounds in CMD, ZMD and SMD. Ruscogenin was not detected in all the samples, and liriopesides B was only found in SMD samples. CMD contained higher ophiopogonin D and ophiopogonin D’, while the other compounds were more abundant in ZMD. Moreover, the anticancer effects of the herbal extracts and selected components against A2780 human ovarian cancer cells were also compared. CMD and ZMD showed similar cytotoxic effects, which were stronger than those of SMD. The effects of MD may be due to the significant anticancer potential of ophiopognin D’ and homoisoflavonoids. These results suggested that there were great differences in the chemical composition and pharmacological activity among CMD, ZMD and SMD; thus, their origins should be carefully considered in clinical application.

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Rapid Screening of Lipase Inhibitors from Ophiopogonis Radix Using High-Performance Thin Layer Chromatography by Two Step Gradient Elution Combined with Bioautographic Method

Cited by 5 publications

References 33 publications

Thin-Layer Chromatography (TLC) in the Screening of Botanicals–Its Versatile Potential and Selected Applications

Thin-Layer Chromatography (TLC) in the Screening of Botanicals–Its Versatile Potential and Selected Applications

Thin-Layer Chromatography (TLC) in Screening of Botanicals – its Versatile Potential and Selected Applications

Comparison of Ophiopogon japonicus and Liriope spicata var. prolifera from Different Origins Based on Multi-Component Quantification and Anticancer Activity

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