Rapid screening of multi-target antitumor drugs by nonimmobilized tumor cells/tissues capillary electrophoresis

Wu, Ruijun; Li, Chen; Liu, Cong; Ren, Jing; Sun, Xiaozhi; Zhang, Sufang; Zou, Juncheng; Ling, Xiaomei

doi:10.1016/j.aca.2018.09.015

Cited by 14 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such a method was employed to use tumor cells/tissues as the interaction phase to allow rapid screening of multi-target antitumor drugs (Fig. 3) [61]. Compared to cell crosslinking, this method can renew the cells every run.…”

Section: T W E N T Y -N I N E P H E N Y L E T H a N O I D G L Y C O S I D E S F R O Mmentioning

confidence: 99%

See 1 more Smart Citation

Recent (2018–2020) development in capillary electrophoresis

Gao

Zhong

2021

Anal Bioanal Chem

View full text Add to dashboard Cite

Development of new capillary electrophoresis (CE) methodology and instrumentation, as well as application of CE to solve new problems, remains an active research area because of the attractive features of CE compared to other separation techniques. In this review, we focus on the representative works about sample preconcentration, separation media or capillary coating development, detector construction, and multidimensional separation in CE, which are judiciously selected from the papers published in 2018-2020.

show abstract

Section: T W E N T Y -N I N E P H E N Y L E T H a N O I D G L Y C O S I D E S F R O Mmentioning

confidence: 99%

“…3 Schematic overflow of non-immobilized tumor cells/tissues CE for rapid screening of multi-target antitumor drugs. For more information, please see [61] Fig. 4 (A [66,69,72] separation channel to the MS (Fig.…”

Section: Mass Spectrometry (Ms)mentioning

confidence: 99%

Recent (2018–2020) development in capillary electrophoresis

Gao

Zhong

2021

Anal Bioanal Chem

View full text Add to dashboard Cite

show abstract

“…As multitarget on cells/tissues, screening multitarget drugs has been explored and being an interesting field . Ling's group introduced a method called immobilized cell CE, which has been employed for rapid screening of antitumor metastasis drugs targeting integrin macrophage antigen‐1 (MAC‐1).…”

Section: Online Screening Of Enzyme Inhibitorsmentioning

confidence: 99%

“…Twenty nine phenylethanoid glycosides from Cistanches Herba were screened, and the binding constants obtained using immobilized cell capillary electrophoresis were well consistent with the order of Glide‐calculated value obtained from the molecular docking study. Thereafter, they expanded the method on the study of drug screening targeting A549 cells . Under the optimum conditions, seven components with binding activity to A549 cells, as well as five of them with specific interaction to tumor tissues, were discovered via the interaction between Aidi injection and tumor cells/tissues.…”

Section: Online Screening Of Enzyme Inhibitorsmentioning

confidence: 99%

See 1 more Smart Citation

Advances in screening enzyme inhibitors by capillary electrophoresis

Wang

Yang

2019

Electrophoresis

View full text Add to dashboard Cite

Capillary electrophoresis (CE) has attracted lots of attention due to its simplicity, low sample consumption, low solvent volume, high resolution, and high speed. Based on these advantages, it has been widely used in enzyme inhibitor screening. There are two main operation modes on enzyme inhibitor screening: off‐line (precapillary enzyme assays) in which process CE was used as an analytical tool; online (in‐capillary enzyme assays) which combined the sample injection, mix, reaction, separation, and detection within a single run. Additionally, diverse of new materials were introduced to immobilize enzyme, which has been coupled with CE for the study of enzyme activity and its inhibitor screening. This review gives an overview of the developments and applications for the CE‐based enzyme inhibitor screening.

show abstract

Multiscale modelling of fluid transport in vascular tumours subjected to electrophoresis anticancer therapies

Fülöp,

Ramírez-Torres,

Penta

2023

Z. Angew. Math. Phys.

View full text Add to dashboard Cite

Electrophoresis facilitated cancer treatment has demonstrated experimental efficacy in enhancing drug delivery within vascularised tumours. However, the lack of realistic mathematical models with direct measurements in the context of electrochemotherapy poses a challenge. We investigate the impact of an applied electric potential on the flow of Darcian-type fluid occurring in two distinct phases: the tumour and healthy regions. We employ the asymptotic homogenisation technique, assuming that the macroscale of the tumour domain is larger than the microscale characterised by vessel heterogeneities. We retain information about the microstructure by encoding information in the homogenised coefficients. We take into account both vascularisation and the microscale variations of the leading order and fine scale electric potential. The resulting effective differential problem reads as a Darcy-type system of PDEs, where the flow is driven by an effective source. The novel model can be used to predict the effect of an applied electric field on cancerous biological tissues, paving a new way of improving current electrochemotherapy protocols.

show abstract

Rapid screening of multi-target antitumor drugs by nonimmobilized tumor cells/tissues capillary electrophoresis

Cited by 14 publications

References 32 publications

Recent (2018–2020) development in capillary electrophoresis

Recent (2018–2020) development in capillary electrophoresis

Advances in screening enzyme inhibitors by capillary electrophoresis

Multiscale modelling of fluid transport in vascular tumours subjected to electrophoresis anticancer therapies

Contact Info

Product

Resources

About