Rapid screening of Musa species for resistance to Fusarium wilt in an in vitro bioassay

Wu, Yuan-li; Peng, Xiaoqun

doi:10.1007/s10658-010-9669-y

Cited by 40 publications

(22 citation statements)

References 22 publications

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“…The mycelia were rinsed three times with 200 mL sterilized distilled water. The conidial density of the suspension in the flask was determined with a hemocytometer (Wu et al, 2010). Before incubation, the conidia suspensions were adjusted by volume with distilled sterilized water in the proportions needed to generate suspensions with the standard concentrations required by the experiments.…”

Section: Plant Materials and Inoculum Suspension Preparationmentioning

confidence: 99%

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A Rapid Seedling Assay for Determining Sweetpotato Resistance to Fusarium Wilt

Yang

Chen

et al. 2018

Crop Science

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Fusarium wilt, caused by Fusarium oxysporum f. sp. batatas (Woolenw.) W. C. Snyder & H. N. Hansen (abbreviated as “Fob”), is a devastating disease that can cause sweetpotato [Ipomoea batatas (L) Lam.] yield losses of up to 50%. A rapid and efficient assay can expedite the selection of Fusarium wilt‐resistant cultivars. A rapid seedling assay was developed to evaluate sweetpotato resistance to Fusarium wilt. Seedlings of three cultivars—JinShan 57 (highly resistant), JinShan 696 (moderately resistant), and XinZhongHua (highly susceptible)—were grown in suspensions containing different conidial concentrations of Fob. Inoculation with a Fob conidial suspension of 1 × 105 conidia mL−1, incubation of inoculated plants at 28°C, and assessment of disease development 12 d after inoculation provided optimal results. Resistance of 28 sweetpotato advanced breeding selections was evaluated in 2014 and 2015 using the rapid method and a conventional field test. These tests confirmed the reliability of the rapid method. This study demonstrates that the new assay is rapid, efficient, and can facilitate screening of large numbers of sweetpotato cultivars and selections for Fob resistance.

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Section: Plant Materials and Inoculum Suspension Preparationmentioning

confidence: 99%

“…A fast, space‐effective, and accurate in vitro bioassay was developed for screening resistance to Fusarium wilt in banana ( Musa spp.) (Wu et al, 2010). Two types of in vitro seedling tests were developed to evaluate the resistance of flax ( Linum usitatissimum L.) against Fusarium wilt.…”

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confidence: 99%

A Rapid Seedling Assay for Determining Sweetpotato Resistance to Fusarium Wilt

Yang

Chen

et al. 2018

Crop Science

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“…A fast and effective method was also developed for screening resistance to Fusarium wilt in banana (Musa spp.) [23]. The effectiveness and the degree of resistance and susceptibility may vary among years or soil type and also can be affected by other soil microbes [20,24].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Sweetpotato (Ipomoea batatas) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea

Paul

Won

Seung-yong

et al. 2020

Plants

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Fusarium wilt and Fusarium surface rot caused by Fusarium oxysporum Schltdl are the major diseases of sweetpotato (Ipomoea batatas) and was surveyed in different locations (Cheongju, Heanam, Iksan, Icheon, Kimje, Nonsan, Yeoungam, and Yeoju) in Korea from 2015 to 2017 in the field, after harvesting and in storehouse. The wilt incidence in the early stage represented 17.9%, 5.9%, and 8.3% in 2015, 2016, and 2017, respectively. Samples were collected, and the causal organism was isolated on potato dextrose agar (PDA). Ten pure cultures were stored at the Sweetpotato Research Laboratory, Bioenergy Crop Research Institute, Muan, Korea. Morphological analysis, along with molecular phylogeny of the sequences of internal-transcribed spacer (ITS) and elongation factor 1-α (EF-1α) genes and their combined phylogenetic analysis, confirmed the isolates as the Fusarium oxysporum Schltdl. Pathogenicity tests were conducted on sweetpotato stems, and storage roots by artificially inoculation methods, and the most virulent isolate was selected as SPL18019. A rapid screening method on 21 selected varieties for resistant variety selection was applied on stems. The Pungwanmi was found resistant to Fusarium wilt, whereas Annobeni was the most susceptible. On the other hand, six varieties were used to test surface rot resistance, and Yulmi and Yesumi were resistant and susceptible, respectively, to Fusarium surface rot.

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“…The first group of assays include the use of fusaric acid to challenge plantlets/explants/multiple shoot clumps under in vitro condition (Wu et al 2010), which has little practical value under present conditions, due to its higher plant material requirement. The biochemical-based assays developed in banana for identification of Foc-resistant lines (Li et al 2011) have not been fully accepted due to reliability issues (Chunyu et al 2011).…”

Section: Discussionmentioning

confidence: 99%

In search of commercially acceptable Panama wilt-resistant natural variants in Ney Poovan banana (MusaAB)

Bohra

Waman

Umesha

et al. 2014

International Journal of Pest Management

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Ney Poovan banana, the most widely cultivated mixed diploid banana, has been reported to be susceptible to Fusarium wilt like many other varieties in the world. The identification of natural variants possessing disease tolerance or resistance is one strategy to prevent losses. The aim of this study was to identify resistant lines in Ney Poovan banana, through extensive field surveys and screening of putative variants using a detached leaf-based challenging technique. The selected lines were screened under field condition to determine their economic feasibility for commercial-scale use. A total of 26 lines were observed to be resistant to the disease, out of which 24 exhibited a commercially acceptable productivity index and five best lines possessing desirable attributes were obtained.

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Rapid screening of Musa species for resistance to Fusarium wilt in an in vitro bioassay

Abstract: In order to accelerate breeding

Cited by 40 publications

References 22 publications

A Rapid Seedling Assay for Determining Sweetpotato Resistance to Fusarium Wilt

A Rapid Seedling Assay for Determining Sweetpotato Resistance to Fusarium Wilt

Occurrence of Sweetpotato (Ipomoea batatas) Wilt and Surface Rot Disease and Determining Resistance of Selected Varieties to the Pathogen in Korea

In search of commercially acceptable Panama wilt-resistant natural variants in Ney Poovan banana (MusaAB)

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