Rapid selection of aptamers based on protein microarray

Fang, Yu; Li, Hui; Sun, Wei; Xu, Danke; He, Fuchu

doi:10.1039/c8ra09232j

“…Secondly, several types of small molecules (metabolites, toxins, and antibiotics) are infeasible targets since they are difficult to conjugate onto a solid carrier [133,134] . To mitigate these issues, many research groups [135–137] have considered the inverse method of heterogeneous SELEX, known as capture‐SELEX, in which the nucleic acid library is immobilized rather than the target. The most popular technique of capture‐SELEX is microarray‐based SELEX.…”

Section: Selex Methodologiesmentioning

confidence: 99%

Development of Better Aptamers: Structured Library Approaches, Selection Methods, and Chemical Modifications

Brown,

Brill,

Amini

et al. 2024

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Systematic evolution of ligands by exponential enrichment (SELEX) has been used to discover thousands of aptamers since its development in 1990. Aptamers are short single‐stranded oligonucleotides capable of binding to targets with high specificity and selectivity through structural recognition. While aptamers offer advantages over other molecular recognition elements in their ease of production, smaller size, extended shelf‐life, and lower immunogenicity, they have yet to show significant success in real‐world applications. By analyzing the importance of structured library designs, reviewing different SELEX methodologies, and the effects of chemical modifications, we provide a comprehensive overview on the production of aptamers for applications in drug delivery systems, therapeutics, diagnostics, and molecular imaging.

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“…[120,132] Secondly, several types of small molecules (metabolites, toxins, and antibiotics) are infeasible targets since they are difficult to conjugate onto a solid carrier. [133,134] To mitigate these issues, many research groups [135][136][137] have considered the inverse method of heterogeneous SELEX, known as capture-SELEX, in which the nucleic acid library is immobilized rather than the target. The most popular technique of capture-SELEX is microarray-based SELEX.…”

Section: Microarray-based Selexmentioning

confidence: 99%

Development of Better Aptamers: Structured Library Approaches, Selection Methods, and Chemical Modifications

Brown,

Brill,

Amini

et al. 2024

Angewandte Chemie

3

0

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Systematic evolution of ligands by exponential enrichment (SELEX) has been used to discover thousands of aptamers since its development in 1990. Aptamers are short single‐stranded oligonucleotides capable of binding to targets with high specificity and selectivity through structural recognition. While aptamers offer advantages over other molecular recognition elements in their ease of production, smaller size, extended shelf‐life, and lower immunogenicity, they have yet to show significant success in real‐world applications. By analyzing the importance of structured library designs, reviewing different SELEX methodologies, and the effects of chemical modifications, we provide a comprehensive overview on the production of aptamers for applications in drug delivery systems, therapeutics, diagnostics, and molecular imaging.

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“…Later the same group reported a novel approach (Figure 2A) that combines Ag10-NPs enrichment and SPR imaging (SPRI) with the SELEX process to evaluate the detection signal (Jia et al, 2018). Subsequently, to solve the problems of complicated manufacturing and instrumentation, this group established a novel microarray SELEX platform (Yu et al, 2019) with a Smart Arrayer and Microarray Scanner to simplify fabrication and experimental operation (Figure 2B).…”

Section: Protein Microarray-based Selectionmentioning

confidence: 99%

The Application of Microfluidic Technologies in Aptamer Selection

Liu¹,

Wang²,

Chan³

et al. 2021

Front. Cell Dev. Biol.

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Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligands by exponential enrichment (SELEX). Numerous SELEX methods have been developed for aptamer selections. However, the conventional SELEX methods still suffer from high labor intensity, low operation efficiency, and low success rate. Thus, the applications of aptamer with desired properties are limited. With their advantages of low cost, high speed, and upgraded extent of automation, microfluidic technologies have become promising tools for rapid and high throughput aptamer selection. This paper reviews current progresses of such microfluidic systems for aptamer selection. Comparisons of selection performances with discussions on principles, structure, operations, as well as advantages and limitations of various microfluidic-based aptamer selection methods are provided.

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Rapid selection of aptamers based on protein microarray

Abstract: We report a novel method for the efficient screening of aptamers from a complex ssDNA library based on a microarray chip, which was named Microarray-SELEX.

Cited by 9 publications

References 36 publications

Development of Better Aptamers: Structured Library Approaches, Selection Methods, and Chemical Modifications

Development of Better Aptamers: Structured Library Approaches, Selection Methods, and Chemical Modifications

Development of Better Aptamers: Structured Library Approaches, Selection Methods, and Chemical Modifications

The Application of Microfluidic Technologies in Aptamer Selection

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