Rapid Self‐Integrating, Injectable Hydrogel for Tissue Complex Regeneration

Hou, Sen; Wang, Xuefei; Park, Sean S.; Jin, Xun; Peter, X.

doi:10.1002/adhm.201500093

Cited by 172 publications

(134 citation statements)

References 29 publications

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“…Chen et al synthesized self-healing hydrogels via dynamic Schiff base reaction between oxidized sodium alginate and N-carboxyethyl chitosan, for delivery of neural stem cells [41]. In addition, there are series of polysaccharide-based self-healing hydrogels reported for culturing or delivering a wide range of cell types, including fibroblasts, HeLa cells, chondrocytes, marrow stem cells, and so on [26,32,36,42,43]. Nonetheless, despite the high cell viability of these self-healing hydrogels, most cells encapsulated in them are observed as invariably rounded shapes without cell-cell communications, even following a long culture period.…”

Section: Introductionmentioning

confidence: 99%

A self-healing hydrogel as an injectable instructive carrier for cellular morphogenesis

2018

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Transplantation of progenitor cells can accelerate tissue healing and regenerative processes. Nonetheless, direct cell delivery fails to support survival of transplanted cells or long-term treatment of vascular related diseases due to compromised vasculature and tissue conditions. Using injectable hydrogels that cross-link in situ, could protect cells in vivo, but their sol-gel transition is time-dependent and difficult to precisely control. Hydrogels with self-healing properties are proposed to address these limitations, yet current self-healing hydrogels lack bio-functionality, hindering the morphogenesis of delivered cells into a tissue structure. Here we establish a gelatin (Gtn)-based self-healing hydrogel cross-linked by oxidized dextran (Odex) as an injectable carrier for delivery of endothelial progenitors. The dynamic imine cross-links between Gtn and Odex confer the self-healing ability to the Gtn-l-Odex hydrogels following syringe injection. The self-healing Gtn-l-Odex not only protects the progenitors from injected shear force but it also allows controllable spatial/temporal placement of the cells. Moreover, owing to the cell-adhesive and proteolytic sites of Gtn, the Gtn-l-Odex hydrogels support complex vascular network formation from the endothelial progenitors, both in vitro and in vivo. This is the first report of injectable, self-healing hydrogels with biological properties promoting vascular morphogenesis, which holds great promise for accelerating the success of regenerative therapies.

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Section: Introductionmentioning

confidence: 99%

A self-healing hydrogel as an injectable instructive carrier for cellular morphogenesis

2018

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“…[15,16] Many forms of SF biomaterials including films, electrospun nanofibers, sponges, and hydrogels have been developed and explored as scaffolds for bone regeneration. [19,20] SF hydrogels were obtained from SF solution based on the transition of the protein conformation from amorphous to intermolecular β-sheet which normally varies from hours to months. [18] Despite of existence of many solid SF scaffolds, injectable SF hydrogels are advantageous in that they can easily fill irregularly shaped bone defects without the need to mold the material shapes in advance.…”

mentioning

confidence: 99%

Self‐Healing Silk Fibroin‐Based Hydrogel for Bone Regeneration: Dynamic Metal‐Ligand Self‐Assembly Approach

Shi

Wang

Zhu

et al. 2017

Adv Funct Materials

229

169

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Despite advances in the development of silk fibroin (SF)-based hydrogels, current methods for SF gelation show significant limitations such as lack of reversible crosslinking, use of nonphysiological conditions, and difficulties in controlling gelation time. In the present study, a strategy based on dynamic metal-ligand coordination chemistry is developed to assemble SF-based hydrogel under physiological conditions between SF microfibers (mSF) and a polysaccharide binder. The presented SF-based hydrogel exhibits shearthinning and autonomous self-healing properties, thereby enabling the filling of irregularly shaped tissue defects without gel fragmentation. A biomineralization approach is used to generate calcium phosphate-coated mSF, which is chelated by bisphosphonate ligands of the binder to form reversible crosslinkages. Robust dually crosslinked (DC) hydrogel is obtained through photopolymerization of acrylamide groups of the binder. DC SF-based hydrogel supports stem cell proliferation in vitro and accelerates bone regeneration in cranial critical size defects without any additional morphogenes delivered. The developed self-healing and photopolymerizable SF-based hydrogel possesses significant potential for bone regeneration application with the advantages of injectability and fit-to-shape molding.

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“…For example, Ma et al presented a self-healing dextran-based hydrogel consisted of multiple-hydrogen-bond units (ureido-pyrimidinone, Upy) modified dextran chains, which can achieve rapid self-integration through the hydrogen bond between Upy units29. Two pieces of self-healed cell-loaded hydrogels which separately loaded with chondrocytes and bone marrow stem cells can form cartilage-bone tissue complex after subcutaneous implantation.…”

mentioning

confidence: 99%

Self-healing polysaccharide-based hydrogels as injectable carriers for neural stem cells

Zhao¹,

Zhao²,

Chen³

et al. 2016

Sci Rep

137

103

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Self-healing injectable hydrogels can be formulated as three-dimensional carriers for the treatment of neurological diseases with desirable advantages, such as avoiding the potential risks of cell loss during injection, protecting cells from the shearing force of injection. However, the demands for biocompatible self-healing injectable hydrogels to meet above requirements and to promote the differentiation of neural stem cells (NSCs) into neurons remain a challenge. Herein, we developed a biocompatible self-healing polysaccharide-based hydrogel system as a novel injectable carrier for the delivery of NSCs. N-carboxyethyl chitosan (CEC) and oxidized sodium alginate (OSA) are the main backbones of the hydrogel networks, denoted as CEC-l-OSA hydrogel (“l” means “linked-by”). Owing to the dynamic imine cross-links formed by a Schiff reaction between amino groups on CEC and aldehyde groups on OSA, the hydrogel possesses the ability to self-heal into a integrity after being injected from needles under physiological conditions. The CEC-l-OSA hydrogel in which the stiffness mimicking nature brain tissues (100~1000 Pa) can be finely tuned to support the proliferation and neuronal differentiation of NSCs. The multi-functional, injectable, and self-healing CEC-l-OSA hydrogels hold great promises for NSC transplantation and further treatment of neurological diseases.

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Rapid Self‐Integrating, Injectable Hydrogel for Tissue Complex Regeneration

Cited by 172 publications

References 29 publications

A self-healing hydrogel as an injectable instructive carrier for cellular morphogenesis

A self-healing hydrogel as an injectable instructive carrier for cellular morphogenesis

Self‐Healing Silk Fibroin‐Based Hydrogel for Bone Regeneration: Dynamic Metal‐Ligand Self‐Assembly Approach

Self-healing polysaccharide-based hydrogels as injectable carriers for neural stem cells

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