Rapid, Sensitive and Selective Analysis of trans-Resveratrol in Rat Plasma by LC–MS–MS

Abstract

A rapid, sensitive and highly selective liquid chromatography/tandem mass spectrometry (LC-MS-MS) using an atmospheric pressure chemical ionization (APCI) method was developed to determine the concentration of trans-resveratrol in rat plasma. Samples were extracted using ethyl acetate after daidzein was added as an internal standard (IS). The main advantages of this method were the high sensitivity (a lower limit of quantification, LLOQ, of 0.1 ng mL -1 ) and the short analysis time (total run time 3.2 min per sample). Separation was performed on a ZORBAX ODS column with a mobile phase that consisted of acetonitrile/water (48:52, v/v). trans-Resveratrol and IS were detected using a triple-quadrupole mass spectrometer in the selective reaction monitoring (SRM) mode with the parent-to-product quantifier transitions [M-H] -m/z 226.8 ? 142.9 and [M-H] -m/z 252.9 ? 252.9 for transresveratrol and IS, respectively. The method was confirmed to be accurate and precise with a linearity range of 0.1-500 ng mL -1 with r [ 0.99. Recoveries for transresveratrol and IS were within 90-101%. The accuracy and precision for the assay were determined by calculating the intra-and inter-batch variation for quality control (QC) samples at four concentration levels with a relative standard deviation (RSD) of \15%. This method was successfully applied to determine trans-resveratrol in rat plasma and proved suitable for pharmacokinetic study after intragastric administration of trans-resveratrol in sustained release granules and normal granules, respectively.

Trans-ε-Viniferin Encapsulation in Multi-Lamellar Liposomes: Consequences on Pharmacokinetic Parameters, Biodistribution and Glucuronide Formation in Rats

“…The method validation was carried out in accordance with the United States Food and Drug Administration [52] and the European Medicines Agency Guidelines [53] as previously described in other pharmacokinetic studies [54][55][56][57][58][59].…”

Synthesis, Characterization, and Evaluation of a Novel Molecularly Imprinted Polymer (MIP) for Selective Quantification of Curcumin in Real Food Sample by UV-Vis Spectrophotometry

“…However, curcumin is often adulterated with other highly toxic dyes such as methanyl yellow [ 8 ] and lead chromate (II) [ 9 ]. Therefore, various detection and quantification methods are employed, such as HPLC [ 10 , 11 ], UV-visible spectrophotometry [ 12 , 13 ], fluorescence [ 14 , 15 ], electrochemistry [ 16 ], etc. The most widely used method is UV-visible spectrophotometry due to its simplicity, high efficiency, and good reliability.…”

Rapid, Highly-Sensitive and Ecologically Greener Reversed-Phase/Normal-Phase HPTLC Technique with Univariate Calibration for the Determination of Trans-Resveratrol

“…Standard method performance requirements and validation protocols have also been set by the Association of Official Agricultural Chemists for the determination of TRV in dietary supplements and dietary ingredients [27]. Some liquid chromatography-mass spectrometry (LC-MS) assays were also utilized for TRV analysis in plant materials, wine samples and plasma samples [28][29][30][31]. Different high-performance thin-layer chromatography (HPTLC) techniques have also been utilized for TRV analysis in a variety of samples such as wine samples, plant extracts, pharmaceutical products, herbal products and polyherbal products [3,4,[32][33][34][35].…”