Rapid Separation of Antioxidants in Food Samples by Coelectroosmotic CE

Hernández-Borges, Javier; Borges‐Miquel, Teresa M.; González-Hernández, G.; Rodríguez-Delgado, M. A.

doi:10.1365/s10337-005-0622-8

Cited by 15 publications

(5 citation statements)

References 37 publications

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“…Figure 8 shows a chromatographic separation of an antioxidant mixture containing kaempferol (1), caffeic acid (2) and gallic acid (3) using nano-LC in the HILIC mode. Antioxidants are compounds that prolong the shelf-life of foods by protecting against deterioration caused by oxidation (28). These compounds are not separated well using RPLC with isocratic elution or without use of acidic mobile phases (29).…”

Section: Chromatographic Characterizationmentioning

confidence: 99%

New Silica Gel-Based Monolithic Column for Nano-Liquid Chromatography, used in the HILIC Mode

Silva

Bottoli

2012

Journal of Chromatographic Science

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This paper describes the synthesis and chromatographic and morphologic characterization of two monolithic silica nano-columns (50 mm i.d.) prepared by sol-gel processes, using hydrophilic interaction (HILIC) mode separations to evaluate their performance. Two types of monoliths were prepared by varying the precursors (tetraethoxysilane or a tetraethoxysilane -methyltrimethoxysilane mixture) and by changing the type of catalyst (urea and acetic acid or ammonium hydroxide). The monoliths were characterized by scanning electron microscopy, thermogravimetric analysis, infrared spectroscopy and nitrogen adsorption -desorption isotherms. The columns were tested for the separation of several mixtures, with the organically modified silica (ormosil) column successfully separating two challenging mixtures using HILIC conditions.

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Section: Chromatographic Characterizationmentioning

confidence: 99%

New Silica Gel-Based Monolithic Column for Nano-Liquid Chromatography, used in the HILIC Mode

Silva

Bottoli

2012

Journal of Chromatographic Science

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“…14 A new separation method, based on coelectrosmotic CE, was developed for simultaneous separation of eight phenolic compounds in cereal and beer samples [47]. Coelectrosmotic CE technique requires that the direction of the electrosmotic flow (EOF) to be the same as the electrophoretic mobility of the ions.…”

Section: Phenols Polyphenols and Pigmentsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

Cifuentes¹

2006

Electrophoresis

150

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“…These sample pretreatment steps are time-consuming and can potentially lead to the degradation of bioactive phytochemicals (e.g., amino acid and biomacromolecules), chemical contamination, and material losses. 3,44,45 For example, alliin in garlic is known to readily convert into allicin by allinase when external mechanical force is applied to garlic bulbs, making it difficult to detect and quantify intact alliin from raw garlic tissues. It has been reported that the conversion of alliin to allicin is rapid and can be completed within 10 s at 23 °C.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Direct Assessment of Phytochemicals Inherent in Plant Tissues Using Extractive Electrospray Ionization Mass Spectrometry

Zhang

Zhu

et al. 2013

J. Agric. Food Chem.

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An ambient pressure ionization mass spectrometric strategy called internal extractive electrospray ionization mass spectrometry (iEESI-MS) has been developed and applied for direct profiling of labile phytochemicals inherent in various native plant tissues, including leaves, roots, and fruits. By passing the electrospray solvent through the plant tissue, a variety of phytochemicals, such as amino acids, sugars (e.g., glucose, sucrose, polysaccharides, etc.), and alkaloids, were continuously extracted from the sample interior, driven toward the natural/cut electro-spraying tip, and vaporized into gaseous ions for mass spectrometric interrogation. Phytochemical patterns obtained by iEESI-MS permit a rapid differentiation between various species of ginkgo plant and strawberry maturity stages, as well as characterization of physiological/pathologic conditions of chlorophytum comosum. Our experimental results further demonstrate that the established iEESI-MS approach is potentially useful for direct phytochemomics studies with minimal biodegradation, allowing elucidation of plant metabolism with high speed, specificity, and simplicity of analysis.

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Rapid Separation of Antioxidants in Food Samples by Coelectroosmotic CE

Cited by 15 publications

References 37 publications

New Silica Gel-Based Monolithic Column for Nano-Liquid Chromatography, used in the HILIC Mode

New Silica Gel-Based Monolithic Column for Nano-Liquid Chromatography, used in the HILIC Mode

Recent advances in the application of capillary electromigration methods for food analysis

Direct Assessment of Phytochemicals Inherent in Plant Tissues Using Extractive Electrospray Ionization Mass Spectrometry

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