Rapid severe sepsis from Pseudomonas fluorescens/putida bacteremia due to skin and soft tissue infection – A case report

Singh, Pratishtha; Montano, Andreas; Bostick, Adam

doi:10.1016/j.amsu.2021.102845

Cited by 11 publications

(4 citation statements)

References 6 publications

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“…A Medline search of ‘( Pseudomonas putida ) AND (bacteremia)’, as well as ‘( Pseudomonas fluorescens ) AND (bacteremia)’ revealed 26 and 34 articles, respectively. These reports included patients with invasive and life-threatening presentations of their respective Pseudomonas species [ 3 , 4 , 5 ].…”

Section: Discussionmentioning

confidence: 99%

An unusual presentation of Pseudomonas citronellolis bacteraemia and Campylobacter gastroenteritis infection in a human – a case report and literature review

Hwang

Rahmati

Gerbino

et al. 2023

Access Microbiology

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Introduction. Pseudomonas citronellolis is an unusual pathogen in humans and has not been extensively described in the scientific literature. Herein, we present a case of bacteremia and septic shock due to Pseudomonas citronellolis following Campylobacter species gastroenteritis in a patient with immunosuppression. Case Presentation. An 80-year-old man with myeloproliferative disorder on ruxolitinib presented with several days of worsening abdominal pain, which rapidly developed into septic shock with multi-organ failure and explosive diarrhea. Gram-negative bacilli observed on Gram staining of his blood culture broth were later identified as Pseudomonas citronellolis and Bacteroides thetaiotaomicron . Repeated abdominal imaging revealed no evidence of intestinal perforation or megacolon. In addition, stool PCR was positive for Campylobacter species. His clinical course improved after 14 days of meropenem with complete resolution of his symptoms and organ failure. Conclusion. P. citronellolis is a rare infection in humans. We postulate that Janus Associated Kinase (JAK) inhibition in myeloproliferative disorders heightened this patient’s risk of bacterial translocation and severe illness in the setting of Campylobacter gastroenteritis. P. citronellolis may be identified more frequently as a pathogen in humans as more advanced diagnostic technologies become increasingly available in clinical microbiology.

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Section: Discussionmentioning

confidence: 99%

An unusual presentation of Pseudomonas citronellolis bacteraemia and Campylobacter gastroenteritis infection in a human – a case report and literature review

Hwang

Rahmati

Gerbino

et al. 2023

Access Microbiology

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“…aeruginosa , Ps. fluorescens , which was observed in 19.3% of the noncompliant samples, can cause acute opportunistic infections in humans and has been reported in clinical samples from the mouth, stomach, lungs and bloodstream as the most common source of infection (Nishimura et al, 2017; Singh et al, 2021). Although it is not suspected of being an aetiological agent of pulmonary disease, a number of reports have identified it in respiratory samples (Dickson et al, 2014; Scales et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

An Italian survey on the microbiological safety of toys containing aqueous media

Briancesco

Paduano

Paradiso

et al. 2022

Journal of Applied Microbiology

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Aims:The purpose of the present investigation is to fill the current gap in information regarding the microbiological quality of toys containing aqueous media and the related risks for users.Methods: Over 18 years, a total of 491 sealed toys containing aqueous media were analysed using conventional microbial culture methods. In addition, molecular methods (PCR/nested RT-PCR, followed by Sanger sequencing) were employed to test for enteric viruses (enteroviruses and adenoviruses) in a subset of toys; subsequently, the infectivity of the positive samples was tested on cell cultures.Results: Of the examined toys, 23.8% were noncompliant with the limits of the European guideline. The most frequently exceeded limits were those for Aerobic bacteria (84.6%), and Pseudomonas aeruginosa (29.9%). Other opportunistic bacterial species that were frequently detected were Stenotrophomonas maltophilia, Pseudomonas fluorescens, Burkholderia cepacia, Sphingomonas paucimobilis and Comamonas acidovorans. In a subset of 28 samples, adenovirus (25%) and enterovirus (11%) genome was also found to be present, although the samples with viral positivity did not show infectivity after inoculation on appropriate cell monolayers. Conclusions:The results indicate a condition of microbial exposure related to the use of toys containing aqueous media. Significance and Impact of Study:The investigation highlights the need for more stringent monitoring during the production, packaging and storage of toys containing aqueous matrices in order to safeguard children's health.

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“…There are several prominent case reports of bacteremia caused by P. aeruginosa in the literature. Most of the patients infected with this bacterium are usually immuno-compromised patients (Singh et al, 2021). Pseudomonas luteola is another species that is now increasingly regarded as a significant contributor to hospital-acquired infections (HAIs).…”

Section: Molecular Methods For Isolationmentioning

confidence: 99%

The efficiency of molecular methods compared to traditional methods in identifying bacteria from blood and cerebrospinal fluid samples

Khaleel¹,

Faisal²,

Altaii³

2023

MJM

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Aims: This study was aimed to directly diagnose bacterial pathogens from clinical specimens by amplifying and sequencing their 16S rRNA gene compared with traditional methods. Methodology and results: A total of 100 specimens, including blood (50 samples) and cerebrospinal fluid CSF (50 samples) were collected from patients hospitalized in Mosul city hospitals for the period from December 2021 to April 2022. Traditional methods based on cultural characteristics and simple biochemical tests were used to detect pathogens from these samples, as followed by hospital laboratories. Bacterial genomic DNA (deoxyribonucleic acid) was extracted directly from the samples and PCR (polymerase chain reaction) was conducted to amplify their 16S rRNA gene using 16S universal primers. PCR products from samples that showed positive results were purified and sent for sequencing. Sequences were compared to counterpart sequences submitted in NCBI to identify the bacterial pathogen. Our results showed that traditional methods for bacterial isolation and identification from CSF detected 5 positive bacterial cultures out of 50 samples tested (10%), while the remaining cultures (90%) did not show any bacterial growth after 7 days of incubation. The 45 negative samples were subjected to molecular identification and it was found that 28/45 (62.2%) successfully amplified 16S rRNA bands indicating the presence of bacterial genomic DNA. Sequencing results showed that the highest prevalent genera in CSF samples were Bacteroides (41.1%), followed by Lactobacillus (17.6%), Pseudomonas (11.7%), Janibacter (11.7%), Achromobacter (11.7%) and Planococcus (5.8%). Traditional methods for bacterial isolation and identification from blood were capable of isolating 2 pathogens from 50 samples tested (4%). When testing the 48 negative samples using molecular methods, 25/48 (52%) successfully amplified 16S rRNA bands. The 16S rRNA sequences belonged to 9 different genera, the most dominant was Pseudomonas (16.6%) and the least dominant was Bacteroides (5.5%). Conclusion, significance and impact of study: Our results clearly show the power of molecular diagnosis compared to traditional methods used at hospitals and show the danger of misdiagnosis. The study showed that the molecular approach is much more efficient than the conventional methods in identifying pathogens as it identified new pathogens that were not previously detected.

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Rapid severe sepsis from Pseudomonas fluorescens/putida bacteremia due to skin and soft tissue infection – A case report

Cited by 11 publications

References 6 publications

An unusual presentation of Pseudomonas citronellolis bacteraemia and Campylobacter gastroenteritis infection in a human – a case report and literature review

An unusual presentation of Pseudomonas citronellolis bacteraemia and Campylobacter gastroenteritis infection in a human – a case report and literature review

An Italian survey on the microbiological safety of toys containing aqueous media

The efficiency of molecular methods compared to traditional methods in identifying bacteria from blood and cerebrospinal fluid samples

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