Rapid, simultaneous and nanomolar determination of pyroglutamic acid and cis-/trans-urocanic acid in human stratum corneum by hydrophilic interaction liquid chromatography (HILIC)–electrospray ionization tandem mass spectrometry

Joo, Kyung Mi; Han, Jeong‐Won; Son, Eui Dong; Nam, Gnu; Chung, Han Young; Jeong, Hye Jin; Cho, Jun Cheol; Lim, Kyung Min

doi:10.1016/j.jchromb.2012.04.008

Cited by 21 publications

(12 citation statements)

References 20 publications

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“…Data for the skin transcriptome. References (67)(68)(69)(70)(71)(72)(73) Acknowledgments: We acknowledge M. Plaut, NIAID project scientist for ADRN, and W. Davidson, NIAID program officer, for review of this manuscript and valuable advice regarding this project; and the following study coordinators at National Jewish Health for assistance in recruiting human participants and performing procedures: P. Taylor, C. Bronchick, and S. Palombi. We also thank J. Streib who orchestrated processing of the laboratory samples.…”

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confidence: 99%

The nonlesional skin surface distinguishes atopic dermatitis with food allergy as a unique endotype

et al. 2019

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Skin barrier dysfunction has been reported in both atopic dermatitis (AD) and food allergy (FA). However, only one-third of patients with AD have FA. The purpose of this study was to use a minimally invasive skin tape strip sampling method and a multiomics approach to determine whether children with AD and FA (AD FA+) have stratum corneum (SC) abnormalities that distinguish them from AD without FA (AD FA−) and nonatopic (NA) controls. Transepidermal water loss was found to be increased in AD FA+. Filaggrin and the proportion of ω-hydroxy fatty acid sphingosine ceramide content in nonlesional skin of children with AD FA+ were substantially lower than in AD FA− and NA skin. These abnormalities correlated with morphologic changes in epidermal lamellar bilayer architecture responsible for barrier homeostasis. Shotgun metagenomic studies revealed that the nonlesional skin of AD FA+ had increased abundance of Staphylococcus aureus compared to NA. Increased expression of keratins 5, 14, and 16 indicative of hyperproliferative keratinocytes was observed in the SC of AD FA+. The skin transcriptome of AD FA+ had increased gene expression for dendritic cells and type 2 immune pathways. A network analysis revealed keratins 5, 14, and 16 were positively correlated with AD FA+, whereas filaggrin breakdown products were negatively correlated with AD FA+. These data suggest that the most superficial compartment of nonlesional skin in AD FA+ has unique properties associated with an immature skin barrier and type 2 immune activation.

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confidence: 99%

The nonlesional skin surface distinguishes atopic dermatitis with food allergy as a unique endotype

et al. 2019

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“…With purified standards, FBPs, including PCA and cis - and trans -UCA, were quantitated using hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILC-MS/MS) for simultaneous determination, according to methods described previously 42 .…”

Section: Methodsmentioning

confidence: 99%

Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients

Pham

Lim

Joo

et al. 2017

Sci Rep

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Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD patients, and 10 healthy normal controls (NCs) and measured contents of FBPs (pyrrolidone carboxylic acid [PCA] and urocanic acid [UCA]) using UPLC-MS/MS, transepidermal water loss (TEWL) and epidermal pH. Compared to NCs, cis-UCA level was increased in CSU lesions (P < 0.05) and decreased in AD lesions (P < 0.01). The cis-to-trans-UCA ratio in SC specimens from CSU patients was significantly greater than those from AD and NC subjects. AD lesions had lower FBP and PCA contents compared to NC skin (both P < 0.001), and higher TEWL and pH compared to CSU lesions. Moreover, cis-UCA, but not trans-UCA, enhanced the IgE-mediated basophil activation, as well as IgE- and calcium-mediated degranulation of LAD-2 cells, in a dose-dependent manner. These findings suggest that increased cis-to-trans UCA ratio in the epidermis is a distinct feature of CSU, which could enhance mast cell degranulation. Modulation of cis-UCA may be a potential target for skin diseases associated with IgE-mediated mast cell degranulation.

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“…Pyroglutamic acid (PGA), a metabolite of glutamine (Gln), and urocanic acid (UCA), an imidazole‐acrylic acid derivative that is produced from histidine, are major components of natural moisturizing factor (NMF) and commonly used as indicators of NMF level in skin stratum corneum (SC) because they are terminal metabolites of amino acids. Joo et al developed and validated a rapid, sensitive, and specific HILIC‐MS/MS method for the simultaneous determination of PGA, cis ‐ and trans ‐UCA in human SC (Joo et al, ). The analytes were extracted phosphate buffer solution (PBS).…”

Section: Hilic‐ms For Targeted Metabolomic Studiesmentioning

confidence: 99%

HILIC‐MS for metabolomics: An attractive and complementary approach to RPLC‐MS

Tang

Zou

Yin

et al. 2014

Mass Spectrometry Reviews

219

105

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Hydrophilic interaction chromatography (HILIC) is an emerging separation mode of liquid chromatography (LC). Using highly hydrophilic stationary phases capable of retaining polar/ionic metabolites, and accompany with high organic content mobile phase that offer readily compatibility with mass spectrometry (MS) has made HILIC an attractive complementary tool to the widely used reverse-phase (RP) chromatographic separations in metabolomic studies. The combination of HILIC and RPLC coupled with an MS detector expands the number of detected analytes and provides more comprehensive metabolite coverage than use of only RP chromatography. This review describes the recent applications of HILIC-MS/MS in metabolomic studies, ranging from amino acids, lipids, nucleotides, organic acids, pharmaceuticals, and metabolites of specific nature. The biological systems investigated include microbials, cultured cell line, plants, herbal medicine, urine, and serum as well as tissues from animals and humans. Owing to its unique capability to measure more-polar biomolecules, the HILIC separation technique would no doubt enhance the comprehensiveness of metabolite detection, and add significant value for metabolomic investigations. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 35:574-600, 2016.

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Rapid, simultaneous and nanomolar determination of pyroglutamic acid and cis-/trans-urocanic acid in human stratum corneum by hydrophilic interaction liquid chromatography (HILIC)–electrospray ionization tandem mass spectrometry

Cited by 21 publications

References 20 publications

The nonlesional skin surface distinguishes atopic dermatitis with food allergy as a unique endotype

The nonlesional skin surface distinguishes atopic dermatitis with food allergy as a unique endotype

Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients

HILIC‐MS for metabolomics: An attractive and complementary approach to RPLC‐MS

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