Rapid simultaneous quantification of five active constituents in rat plasma by high-performance liquid chromatography/tandem mass spectrometry after oral administration of Da-Cheng-Qi decoction

Simultaneous quantification of multiple active components from Xiexin decoction in rat plasma by LC‐ESI‐MS/MS: application in pharmacokinetics

“…In the previously reported pharmacokinetic studies of XXD, only two to five components of one type or three components (baicalin, rhein, and berberine; Xu et al, 2008) were determined in plasma and the more plasma sample quantity (200 mL) was required, but the LLOQ (155.8 ng/ mL) was higher than that in this experiment. In the present study, a sensitive LC-MS/MS method for simultaneous determination of 11 components from Xiexin decoction in rat plasma after a simple single-step protein precipitation is established.…”

“…These include high-performance liquid chromatography (HPLC) and liquid chromatography/tandem mass spectrometry (LC-MS/MS) methods for the determination of alkaloids (Tan et al, 2007;Kim et al, 2007), flavonoids Zuo et al, 2003;Li et al, 2006;Kim et al, 2006) and anthraquinones Deng et al, 2008) in plasma. Only one study using the HPLC method to simultaneously determine alkaloids, flavonoids and anthraquinones in plasma has been published, but in this study, only three components (baicalin, rhein and berberine) were analyzed (Xu et al, 2008). Currently, no studies on the simultaneous quantification of the three types of multiple components of XXD in vivo exist.…”

Effect of Da-Cheng-Qi decoction on pancreatitis-associated lung injury in patients and anti-inflammatory responses in rat models

“…), Zhishi (Fructus Aurantii Immaturus), and Mangxiao (Natrii Sulfas), with crude herbs mixed in a weight ratio of 1:1:1:1. Although the aqueous extract of the formula contains a large amount of compounds, pharmacokinetic studies from our laboratory and others showed that rhein, emodin, magnolol, honokiol, aloe emodin, naringin, naringenin, hesperidin, rheochrysidin, and chrysophanol are 10 main components detected in serum after oral administration of DCQD (Katakai et al, 2002;Wenfu et al, 2008;Xu et al, 2008;Yu et al, 2009). Several components of DCQD have already been demonstrated to be involved in this effect.…”

Development of liquid chromatography/mass spectrometry methods for the quantitative analysis of herbal medicine in biological fluids: a review

“…The development of analytical methods for the analysis of bioactive components of herbal medicine in biological fl uids such as plasma and urine is not an easy task, primarily due to the complex nature of the biological matrices, numbers of bioactive compounds and their metabolites, and the very low concentration of these compounds present in biological fl uids (Xu et al, 2008).…”

“…In particular pharmacokinetic and pharmacodynamic profi les of bioactive compounds responsible for the therapeutic eff ects need to be established to support the clinical claims. This is a diffi cult and complex task, which becomes more so when multiple herbs are used, as frequently occurs in Chinese herbal medicine formulations (Yu et al, 2007a;Yu et al, 2007b;Xu et al, 2008). Liquid chromatographymass spectrometry (LC-MS) is the most promising technique for the measurement of bioactive compounds and their metabolites in blood/plasma due to the inherent high selectivity aff orded through both of the hyphenated techniques.…”