Rapid simultaneous quantification of immunosuppressants in transplant patients by turbulent flow chromatography combined with tandem mass spectrometry

Ceglarek, Uta; Lembcke, Jan; Fiedler, Georg Martin; Werner, M.; Witzigmann, Helmut; Hauss, Johann; Thiery, Joachim

doi:10.1016/j.cccn.2004.03.017

Cited by 116 publications

(52 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Under these simplified chromatographic conditions, CsA and CsD co-eluted with the unspecified, more hydrophilic metabolites of CsA; one single peak was found and integrated in both MRM traces ( Figure 1C, D), both in calibration samples and patient samples. The retention time of this single peak was approximately 2.1 min, which is in agreement with previously described methods for the quantification of CsA employing CsD as the internal standard and the same mass transitions as applied here (2)(3)(4)(5). The quantitative results obtained in this series using limited chromatography differed markedly from those obtained with extended chromatography and from the immunoassay results (Table 1).…”

supporting

confidence: 89%

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry

Vogeser

Spohrer²

2005

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized in vivo; in liquid chromatography-tandem mass spectrometry respective metabolites can give rise to both parent and product ions that are isobaric with ions commonly used for the detection of cyclosporin A and cyclosporin D, respectively. In this article it is demonstrated that limited chromatography with co-elution of such metabolites together with cyclosporin A and cyclosporin D can lead to incorrect results.Keywords: cyclosporin A; cyclosporin D; liquid chromatography-tandem mass spectrometry; specificity.In tandem-mass spectrometry the detection of target analytes is based on their physical decomposition behavior; the molecular mass-specific selection of an intact precursor ion of an analyte and of the respective product ion results in very high analytical specificity. This high degree of specificity can limit the requirements of sample preparation and chromatography and may enable ''high-throughput'' routine methods. However, the specificity of tandem-mass spectrometry should not be assumed to be absolute and unlimited. Given the complexity of human body fluids as a matrix and the extensive metabolization of certain target analytes, the specificity of liquid chromatography-tandem mass spectrometry (LC-MS/MS) may be a critical issue. This is demonstrated by an observation reported here.With the intention of switching the quantification of whole blood cyclosporin A (CsA) from immunoassay technology to LC-MS/MS, we decided to modify the LC-MS/MS method that we currently use for the quan-

show abstract

supporting

confidence: 89%

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry

Vogeser

Spohrer²

2005

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

show abstract

“…Several HPLC methods with ultraviolet (UV) detection or mass spectrometry (MS) are available for measurement of SRL and SDZ-RAD individually (10 -16 ), and liquid chromatography (LC) methods using MS or tandem MS allow simultaneous measurement of these compounds in combination with other immunosuppressants (17)(18)(19)(20)(21)(22). The purported advantage of LC-MS methods over LC-UV methods is the ability to monitor multiple drugs within the same analytical run.…”

Section: Simultaneous Measurement Of Sirolimus and Everolimus In Wholmentioning

confidence: 99%

Simultaneous Measurement of Sirolimus and Everolimus in Whole Blood by HPLC with Ultraviolet Detection

Khoschsorur

2005

Clinical Chemistry

View full text Add to dashboard Cite

146 -254 g/L. As long as more valid data are lacking, serum concentrations of 146 -254 g/L can be considered as a preliminary target range for TDM of aripiprazole. Moreover, it should be stressed that because pure and authentic reference materials for aripiprazole and its metabolite were not available, the accuracy of the method described here remains uncertain.We thank Sandra Heller for skillful technical assistance. Note Added in Proof:The manufacturer (Bristol-Myers Squibb) has subsequently provided us with pure aripiprazole. We prepared samples containing 50, 250, 500, and 1000 g/L and assayed them with our method. The results obtained were 49, 240, 488, and 995 g/L.References

show abstract

“…Recent data indicate that chronic inflammation may lead to atherosclerosis in healthy populations. It has been suggested that systemic markers of inflammation such as C-reactive protein (CRP) and interleukin-6 (IL-6) are important predictors of cardiovascular risk, and measurement of their concentrations may increase the predictive value of traditional lipid screening (8,9 ). CRP assayed by high-sensitivity methods enables the evaluation of low-grade inflammation in early atherosclerosis.…”

Section: Evaluation Of 3 Internalmentioning

confidence: 99%

Evaluation of 3 Internal Standards for the Measurement of Cyclosporin by HPLC–Mass Spectrometry

Taylor

Brown²,

Cooper

et al. 2005

Clinical Chemistry

View full text Add to dashboard Cite

patients, whereas polymyositis, esophageal dysmotility, and scleroderma were found mainly in MCTD patients. In one sample (obtained from a SLE patient), no antibodies to recombinant protein A, protein C, and the 70 kD protein could be identified. In this patient, anti-RNP antibodies were revealed only when a purified antigen was used.Antibodies to (a) RNP-A, RNP-C, and RNP-70 kD; (b) RNP-A and RNP-70 kD; (c) RNP-A and RNP-C; (d) RNP-C and RNP-70 kD; (e) RNP-A only; (f) RNP-C only; and (g) RNP-70 kD only were found in, respectively, 9, 0, 3, 1, 0, 0, and 0 of the 14 SLE patients and in 13, 2, 2, 1, 0, 0, and 2 of the 20 MCTD patients. These distributions were comparable in SLE and MCTD. The patients with chronic idiopathic urticaria, rheumatoid arthritis, or parotitis had antibodies to, respectively, RNP-70 kD only, RNP-A and RNP-C, and RNP-70 kD only. Table 1 contains a survey of

show abstract

Rapid simultaneous quantification of immunosuppressants in transplant patients by turbulent flow chromatography combined with tandem mass spectrometry

Cited by 116 publications

References 25 publications

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry

Simultaneous Measurement of Sirolimus and Everolimus in Whole Blood by HPLC with Ultraviolet Detection

Evaluation of 3 Internal Standards for the Measurement of Cyclosporin by HPLC–Mass Spectrometry

Contact Info

Product

Resources

About