Rapid Single-cell Detection and Identification of Bacteria by Using Surface-enhanced Raman Spectroscopy

Review on SERS of Bacteria

“…Zhou et al [ 38 , 39 , 40 ] prepared an external colloid on bacteria using a different methodology. In their procedure, they added a silver nitrate solution to a sample of bacteria.…”

“…A sample of the suspension was pipetted onto a glass slide, or poly- l -lysine coated glass slide, prior to obtaining SERS spectra. Poly- l -lysine coated glass slides had to be used to immobilize Gram-positive bacteria [ 39 , 40 ]. Figure 6 a shows a TEM image of an E. coli cell showing that the Ag NPs had formed on the cell wall and that they were in intimate contact with the cell wall [ 38 ].…”

Vibrational spectroscopic analysis of blood for diagnosis of infections and sepsis: a review of requirements for a rapid diagnostic test

“…Ⅹ Sensitivity of 81% and specificity of 41% for predicting mortality in ED patients with infections 111 . Volume of blood 20-30mL per set 132 <5mL 40 1-5µL 77 1-10µL 72,133 Culture Time 2-7 days 35,87,134 24 hours 45 6-24 hours 77, 135 6-24 hours 72,73,133 Drying Time N/A N/A <30 minutes 78,135 0-60 minutes 73,133 Analysis Time* <2 hours** 35 4-16 hours 40 <20 minutes 136,137 <30minutes 138,139 Positive Predictive Value <55% for 1 culture, <98% for 2 140 30-57% 56…”

Highly accurate random DNA sequencing using inherent interlayer potential traps of bilayer MoS2nanopores

“…Achieving a good detection rate and accuracy at picomolar DNA concentrations thus requires optimized surface charge interaction around the nanopore. Few works have been performed to improve sensing efficiency at low concentration [11][12][13] . Pathogen identification and DNA oligo sensing by Surface Enhanced Raman Spectroscopy has been performed [11][12] .…”

“…Few works have been performed to improve sensing efficiency at low concentration [11][12][13] . Pathogen identification and DNA oligo sensing by Surface Enhanced Raman Spectroscopy has been performed [11][12] . Low concentration analyte detection has been achieved by employing additional techniques like dielectrophoretic (DEP) trapping to increase the electrophoretic pull exerted on the molecular strand to be sensed 13 .…”