Rapid thermal lysis of cells using silicon–diamond microcantilever heaters

Privorotskaya, Natalya L.; Liu, Yi Shao; Lee, Jungchul; Zeng, Hongjun; Carlisle, John A.; Radadia, Adarsh D.; Millet, Larry J.; Bashir, Rashid; King, William P.

doi:10.1039/b923791g

Cited by 58 publications

(47 citation statements)

References 27 publications

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“…[22][23][24][25][26][27][28] Yet in the observed event, our approach discussed in this article has a key difference from the commonly used techniques; our technique specifically detects microbial quinones by heating, i.e., the thermal lysis of microbes 29,30 and can acquire accurate information about their respiration mode, as seen in Fig. 4.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Investigation of Isoprenoid Quinone Productions byShewanella oneidensisMR-1 Detected by Its Destructive Adsorption on an Indium-Tin-Oxide Electrode

Morishita

Higashimae

Nomoto

et al. 2016

J. Electrochem. Soc.

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Ubiquinone and menaquinone play important roles in microbial respiratory chains. S. oneidensis is a facultative anaerobe, and it has been known that this microbe requires menaquinone (MK) and/or methylmenaquinone (MMK) to reduce inorganic and organic compounds, such as Fe(III), Mn(IV), fumarate, and nitrate, under anaerobic conditions. In this article, we show that voltammetry is useful to quantify ubiquinone (UQ), MK and MMK located in the cytoplasmic membrane simply by heating the microbe (thermal lysis) deposited on an indium-tin oxide electrode. It was found that the microbe predominantly produced UQ under aerobic conditions, while the production was switched to that of MMK under anaerobic conditions. This transition occurred at a dissolved oxygen concentration of ∼0.3 mg L −1 . It was also found in this study that the total quinone concentration (UQ + MK + MMK) in a single cell was constant at all stages of the culture in a nutrition broth, suggesting that the cytoplasmic membrane was saturated with the quinones. The reduction of Fe(III) citrate with S. oneidensis was also studied. It was found that this microbe decreased the UQ and MMK levels to 45% and 39% of the initial values, respectively, after finishing the reduction.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Investigation of Isoprenoid Quinone Productions byShewanella oneidensisMR-1 Detected by Its Destructive Adsorption on an Indium-Tin-Oxide Electrode

Morishita

Higashimae

Nomoto

et al. 2016

J. Electrochem. Soc.

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“…Although previous literature suggests that shear forces at least an order of magnitude higher might be needed for the highly deformable RBC (27), we propose that it is a combination of these SAW-induced shear forces, coupled with the rapidly changing acoustic pressure fields and radiation pressures, which enables the rapid SAW lysis (which takes less than 3 s). Although the dissipation of acoustic energy in the droplet will generate heat, the temperature of blood droplets during the SAW lysis only rises to 38°C, confirming that the lysis process was not due to an increase in temperature (28).…”

Section: Resultsmentioning

confidence: 72%

Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologies

Reboud

Bourquin

Wilson

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

188

161

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Ultrasonics offers the possibility of developing sophisticated fluid manipulation tools in lab-on-a-chip technologies. Here we demonstrate the ability to shape ultrasonic fields by using phononic lattices, patterned on a disposable chip, to carry out the complex sequence of fluidic manipulations required to detect the rodent malaria parasite Plasmodium berghei in blood. To illustrate the different tools that are available to us, we used acoustic fields to produce the required rotational vortices that mechanically lyse both the red blood cells and the parasitic cells present in a drop of blood. This procedure was followed by the amplification of parasitic genomic sequences using different acoustic fields and frequencies to heat the sample and perform a real-time PCR amplification. The system does not require the use of lytic reagents nor enrichment steps, making it suitable for further integration into lab-ona-chip point-of-care devices. This acoustic sample preparation and PCR enables us to detect ca. 30 parasites in a microliter-sized blood sample, which is the same order of magnitude in sensitivity as lab-based PCR tests. Unlike other lab-on-a-chip methods, where the sample moves through channels, here we use our ability to shape the acoustic fields in a frequency-dependent manner to provide different analytical functions. The methods also provide a clear route toward the integration of PCR to detect pathogens in a single handheld system. phononic crystal | surface acoustic waves | nucleic acid amplification test | mechanical cell lysis A coustic waves contain a mechanical energy that can be used to manipulate fluids, cells, and samples (1). A range of ultrasonic transducers have previously been developed, including those using surface acoustic wave (SAW) devices, as a practical solution to actuate fluids on microfluidic chips (2, 3). SAWs have the advantage that, despite using low powers, the energy is concentrated at the interface between the fluid and the substrate, enabling a range of fluid manipulations on a chip. Despite this ability to implement low power microfluidics, one potential disadvantage of using a SAW chip is the relatively high cost of the piezoelectric wafer. In an alternative configuration, the SAW can be coupled into a disposable superstrate (Fig. 1A) placed on the surface of the piezoelectric chip (4, 5), thus providing a low cost technology.Using such superstrates, we have recently demonstrated an alternative and improved method for performing complex fluid manipulations in which the ultrasonic waves are coupled into phononic lattices. Importantly, the functionality of such phononic structures is dependent upon the acoustic frequency. By using phononics to locally shape the acoustic fields and by switching between different ultrasonic wavelengths, we have designed tools capable of enabling different fluid manipulations on the disposable superstrate (5-7).In this paper we show the implementation of nucleic acid based tests (NATs) on a microfluidic chip to demonstrate the potential of phon...

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“…In some biochemical assays, it is desirable to analyze the content of cells such as intracellular proteins, organelles, chromosomes, RNA, and DNA. To this end, buffers containing chaotropic salts as cell lysing agents can be used, [ 149 ] alternatively to lysing cells using heated surfaces, [ 150 ] centrifugation, [ 151 ] or inductive heating. [ 152 ] …”

Section: Cell Sortingmentioning

confidence: 99%

Microfluidic Chips for Point‐of‐Care Immunodiagnostics

2011

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We might be at the turning point where research in microfluidics undertaken in academia and industrial research laboratories, and substantially sponsored by public grants, may provide a range of portable and networked diagnostic devices. In this Progress Report, an overview on microfluidic devices that may become the next generation of point-of-care (POC) diagnostics is provided. First, we describe gaps and opportunities in medical diagnostics and how microfluidics can address these gaps using the example of immunodiagnostics. Next, we conceptualize how different technologies are converging into working microfluidic POC diagnostics devices. Technologies are explained from the perspective of sample interaction with components of a device. Specifically, we detail materials, surface treatment, sample processing, microfluidic elements (such as valves, pumps, and mixers), receptors, and analytes in the light of various biosensing concepts. Finally, we discuss the integration of components into accurate and reliable devices.

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Rapid thermal lysis of cells using silicon–diamond microcantilever heaters

Cited by 58 publications

References 27 publications

Electrochemical Investigation of Isoprenoid Quinone Productions byShewanella oneidensisMR-1 Detected by Its Destructive Adsorption on an Indium-Tin-Oxide Electrode

Electrochemical Investigation of Isoprenoid Quinone Productions byShewanella oneidensisMR-1 Detected by Its Destructive Adsorption on an Indium-Tin-Oxide Electrode

Shaping acoustic fields as a toolset for microfluidic manipulations in diagnostic technologies

Microfluidic Chips for Point‐of‐Care Immunodiagnostics

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