Rapid ultra‐high‐performance liquid chromatography/quadrupole time‐of‐flight tandem mass spectrometry and selected reaction monitoring strategy for the identification and quantification of minor spinacetin derivatives in spinach

Singh, Jashbir; Jayaprakasha, G.K.; Patil, Bhimanagouda S.

doi:10.1002/rcm.7967

Cited by 13 publications

(9 citation statements)

References 39 publications

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“…Thirteen compounds were tentatively identified, including twelve flavonoids (glucuronides and acylated di-and triglycosides of methylated and methylene dioxide derivatives of 6-oxygenated flavanols) and one phenolic acid (p-coumaric acid derivative). All tentatively identified flavonoids have already been extensively described in spinach by other authors [64][65][66][67] at m/z 359) the most characteristics aglycones, while their presence is even considered a determining factor for spinach quality [65]. Two other flavonoids have also been tentatively identified as 5,3 ,4 -trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4 -glucuronide and 5,4 -dihydroxy-3,3 -dimethoxy-6:7-methylenedioxiflavone-4 -glucuronide [64][65][66][67].…”

Section: Phenolic Compositionmentioning

confidence: 67%

“…All tentatively identified flavonoids have already been extensively described in spinach by other authors [64][65][66][67] at m/z 359) the most characteristics aglycones, while their presence is even considered a determining factor for spinach quality [65]. Two other flavonoids have also been tentatively identified as 5,3 ,4 -trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4 -glucuronide and 5,4 -dihydroxy-3,3 -dimethoxy-6:7-methylenedioxiflavone-4 -glucuronide [64][65][66][67]. The presence of quercetin, isorhamnetin, apigenin, luteolin, among others (the most common flavonols found in plants) has been also reported in spinach (in different plant parts and maturity stages), but their presence is in very low amounts, thus they were not identified in the samples studied herein.…”

Section: Phenolic Compositionmentioning

confidence: 67%

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The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.)

et al. 2019

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In the present study, the effect of biostimulants application on the nutritional quality and bioactive properties of spinach cultivated in protected environment under water stress conditions was evaluated. For this purpose, four commercially available biostimulant products (Megafol (MEG), Aminovert (AM), Veramin Ca (V), Twin Antistress (TA), and two spinach genotypes (Fuji F1 and Viroflay) were tested under two irrigation regimes (normal irrigation (W+), and water-holding (W–). Fat and carbohydrates content was favored by water stress when Megafol (MEGW+) and Veramin (VW+) were applied on Fuji plants, while calorific value was also increased by MEGW+ treatment. In contrast, protein and ash content increased when AMW– and TAW+ were applied on Viroflay plants. Raffinose and glucose were the most abundant sugars, followed by sucrose and fructose, with the highest contents recorded for Fuji plants when AMW+ (fructose, glucose and total carbohydrates), CW– (sucrose), and TAW– (raffinose) treatments were applied. Regarding organic acids, oxalic and malic acid which had the highest contents for the TAW– (Viroflay plants) and AMW– (Fuji plants) treatments, respectively. α- and γ-tocopherol were the only isoforms detected with MEGW– and VW– inducing the biosynthesis of α-tocopherol, while AMW+ increased γ-tocopherol content in Fuji plants. The main fatty acids were α-linolenic and linoleic acids which were detected in the highest amounts in AMW–, AMW+, and TAW+ the former and in AMW–, VW–, and CW+ the latter. Regarding phenolic compounds content, peak 12 (5,3′,4′-Trihydroxy-3-methoxy-6:7-methylenedioxyflavone-4′-glucuronide) was the most abundant compound, especially in Viroflay plants under normal irrigation and no biostimulants added (CW–). The antioxidant and cytotoxic activity of the tested samples did not show promising results when compared with the positive controls, while a variable antibacterial activity was recorded depending on the tested biostimulant, irrigation regime and genotype. In conclusion, a variable effect of the tested biostimulants and irrigation regimes was observed on bioactive properties and chemical composition of both spinach genotypes which highlights the need for further research in order to make profound conclusions regarding the positive effects of biostimulants under water stress conditions.

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Section: Phenolic Compositionmentioning

confidence: 67%

Section: Phenolic Compositionmentioning

confidence: 67%

The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.)

et al. 2019

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“…The solvent gradient was 0–2% B (10 min), 2–45% B (12 min), 45–60% B (5 min), 60–90% B (4 min), 90–0% B (3 min) and 5 min isocratic at 100% A. Mass spectral analyses were performed in positive ionization mode according to our previous methodology …”

Section: Methodsmentioning

confidence: 88%

Effect of harvest time on the levels of phytochemicals, free radical‐scavenging activity, α‐amylase inhibition and bile acid‐binding capacity of spinach (Spinacia oleracea)

2018

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“…Flavonoids were identified in onion extract using Agilent 1290 liquid chromatography (Santa Clara, CA, USA) coupled to a maXis Impact high-resolution mass spectrometer (Bruker Daltonics, Billerica, MA, USA) according to our published paper [ 67 ]. Briefly, a chromatographic resolution was achieved by column Zorbax Eclipse plus (1.8 μm; 50 × 2.1 mm) with a gradient solvent system, (A) 0.1% formic acid in water, and (B) 0.1% formic acid in acetonitrile with a flow rate of 0.2 mL/min at 30 °C.…”

Section: Methodsmentioning

confidence: 99%

Comparative Metabolomics Profiling of Polyphenols, Nutrients and Antioxidant Activities of Two Red Onion (Allium cepa L.) Cultivars

Metrani

Singh

Acharya

2020

Plants

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Onion is among the most widely cultivated and consumed economic crops. Onions are an excellent dietary source of polyphenols and nutrients. However, onions phytonutrient compositions vary with cultivars and growing locations. Therefore, the present study involved the evaluation of polyphenol, nutritional composition (proteins, nitrogen, and minerals), sugars, pyruvate, antioxidant, and α-amylase inhibition activities of red onion cultivars, sweet Italian, and honeysuckle grown in California and Texas, respectively. The total flavonoid for honeysuckle and sweet Italian was 449 and 345 μg/g FW, respectively. The total anthocyanin for honeysuckle onion was 103 μg/g FW, while for sweet Italian onion was 86 μg/g FW. Cyanidin-3-(6”-malonoylglucoside) and cyanidin-3-(6”-malonoyl-laminaribioside) were the major components in both the cultivars. The pungency of red onions in honeysuckle ranged between 4.9 and 7.9 μmoL/mL, whereas in sweet Italian onion ranged from 8.3 to 10 μmoL/mL. The principal component analysis was applied to determine the most important variables that separate the cultivars of red onion. Overall results indicated that total flavonoids, total phenolic content, total anthocyanins, protein, and calories for honeysuckle onions were higher than the sweet Italian onions. These results could provide information about high quality and adding value to functional food due to the phytochemicals and nutritional composition of red onions.

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Rapid ultra‐high‐performance liquid chromatography/quadrupole time‐of‐flight tandem mass spectrometry and selected reaction monitoring strategy for the identification and quantification of minor spinacetin derivatives in spinach

Abstract: UHPLC interfaced with HR-QTOFMS in combination with SRM provides a rapid, reliable and highly sensitive method for the identification of flavonoids, and potentially other bioactive compounds, in a complex matrix.

Cited by 13 publications

References 39 publications

The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.)

The Effects of Biostimulants, Biofertilizers and Water-Stress on Nutritional Value and Chemical Composition of Two Spinach Genotypes (Spinacia oleracea L.)

Effect of harvest time on the levels of phytochemicals, free radical‐scavenging activity, α‐amylase inhibition and bile acid‐binding capacity of spinach (Spinacia oleracea)

Comparative Metabolomics Profiling of Polyphenols, Nutrients and Antioxidant Activities of Two Red Onion (Allium cepa L.) Cultivars

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