Rapid Urease Test, Touch Cytology and Histopathologic Assessment in Determining Infection by Helicobacter pylori in Outpatient Setting

Abstract

Helicobacterpylori (HP) is a common cause of gastric infection with serious consequences which is detected by different methods. This study aimed at comparing the diagnostic value of Rapid Urease Test (RUT), Touch Cytology (TC) and histopathologic assessment in outpatients setting. In this cross-sectional study, 51 candidates for upper gastrointestinal endoscopy were recruited in Tabriz Imam Khomeini Teaching Centre in a 24 month period of time. Three biopsy specimens were obtained from gastric antrum during endoscopic intervention. The RUT, TC and histopathologic assessment were performed on each biopsy specimen in each patient. Definite infection by HP was considered when at least 2 out of 3 tests indicated presence of infection. Fifty one patients, 29 females and 22 males with a mean age of 40.10 +/- 12.54 (range: 18-72) years enrolled in this study. Infection by HP was definite in 41 cases (80.4%). The infection rates by RUT, TC and histopathologic examination were 82.4, 82.4 and 76.5%, respectively. The sensitivity, specificity and accuracy of RUT, TC and histopathologic assessment were 92.7, 60 and 66.75%; 100, 90 and 98% and 95.1, 100 and 96.1%, respectively. There were significant agreements between outcomes of the three methods in diagnosis of infection by HP. In conclusion, TC was the most sensitive and histopathologic assessment was the most specific method in diagnosis of infection by HP in outpatient setting. The diagnostic value of RUT was rather low in this regard.

Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis

“…Cryptococcus neoformans was identified by glossy, mucoid, and cream-colored colonies [19] cultured on Agar Niger and Agar Sabouraud (3 days at room temperature). Species confirmation was performed by positive urease test [19]. The protocol was approved by the Institutional Review Board of “Universidade de São Paulo” Medical School and of “Instituto Emílio Ribas.”…”

Methylation Status of CDH1 Gene in Gastric Biopsy Specimens and Its Association with H. Pylori Infection

“…Helicobacter pylori colonize the human gastrointestinal tract and has been acknowledged as contributing to the development of gastro duodenal disorders such chronic active gastritis, peptic ulcers, and gastric cancer. It has been classified by the International Agency for Research on Cancer (IARC) as a grade-I carcinogen (Ohata et al, 2004;Ahsan et al, 2008;Fakhrjou et al, 2011). Numerous publications have revealed a connection between certain gene promoter methylation and H. pylori infection (Perri et al, 2007;Kaise et al, 2008).…”

Investigation of the association between glaucoma andHelicobacter pyloriinfection using the14C-urea breath test

“…Invasive methods include histopathological examination gastric mucosal samples, polymerase chain reaction, culture methods, and the rapid urease test. Noninvasive methods include serological tests, 14 C-urea breath test (14C-UBT), and H. pylori antigen level measurements in stools (12) . Accurate diagnostic rates of 80%-84% have been reported for noninvasive tests by the serological method; however, tests can be positive for 6-12 months after H. pylori eradication; thus, positivity does not necessarily confirm active infection.…”