2020
DOI: 10.1016/j.tmaid.2020.101632
|View full text |Cite
|
Sign up to set email alerts
|

Rapid viral diagnosis and ambulatory management of suspected COVID-19 cases presenting at the infectious diseases referral hospital in Marseille, France, - January 31st to March 1st, 2020: A respiratory virus snapshot

Abstract: Total word count: 1,867

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

0
111
0
1

Year Published

2020
2020
2022
2022

Publication Types

Select...
7
2

Relationship

6
3

Authors

Journals

citations
Cited by 118 publications
(112 citation statements)
references
References 19 publications
0
111
0
1
Order By: Relevance
“…Each patient benefited from two naso-pharyngeal swab samplings, one per nostril. VitaPCR™ SARS-CoV-2 was systematically compared to our routine in-house real-time RT-PCR as reference method [ 8 , 9 ].…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Each patient benefited from two naso-pharyngeal swab samplings, one per nostril. VitaPCR™ SARS-CoV-2 was systematically compared to our routine in-house real-time RT-PCR as reference method [ 8 , 9 ].…”
Section: Methodsmentioning
confidence: 99%
“…For our routine assay, automated nucleic acid extraction was performed using a KingFisher™ Flex system (Thermo Fisher Scientific), following the manufacturer's instructions. Our routine SARS-CoV-2 RT-PCR assay, that targets the envelope protein E-encoding gene, was performed as previously reported [ 8 ]. Besides, PCR targeting the human β-actin gene was performed to check the quality of DNA extracts [ 9 ].…”
Section: Methodsmentioning
confidence: 99%
“…Testing of respiratory samples were performed using the FTD Respiratory pathogens 21 (Fast Track Diagnosis, Luxembourg), the Biofire FilmArray Respiratory panel 2 plus (Biomérieux, Marcy-l'Etoile, France), the Respiratory Multi Well System r-gene (Argene, BioMérieux), or the GeneXpert Xpert Flu/RSV (Cepheid, Sunnyvale, CA) assays, or by one-step simplex real-time quantitative RT-PCR amplifications as previously reported ( Hoang et al, 2019 ). Diagnosis by reverse transcription-PCR of SARS-CoV-2 infection was performed as previously described ( Amrane et al, 2020 ). This study retrospectively analyzed patients’ data issued from the hospital information system (RGPD/APHM 2019-73).…”
Section: Methodsmentioning
confidence: 99%
“…Some discharged patients were also sampled during follow-up at our out-patients department when possible. SARS-CoV-2 RNA was assessed by real-time reverse transcription-PCR using a hydrolysis probe-based system that targets the gene encoding the envelope (E) protein [21], as previously described [21,22]. Negative results for viral RNA detection were defined as those with a cycle threshold (Ct) value ≥ 35.…”
Section: Pcr Assaymentioning
confidence: 99%