Rapid volumetric imaging with Bessel-Beam three-photon microscopy

Chen, Bingying; Huang, Xiaoshuai; Gou, Dongzhou; Zeng, Jianzhi; Chen, Guoqing; Pang, Meijun; Hu, Yanhui; Zhao, Zhe; Zhang, Yunfeng; Zhou, Zhuan; Wu, Haitao; Cheng, Heping; Zhang, Zhigang; Xu, Chris; Li, Yulong; Chen, Liangyi; Wang, Aimin

doi:10.1364/boe.9.001992

Cited by 71 publications

(51 citation statements)

References 23 publications

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“…Since the first demonstrations of 3PM for in vivo brain imaging (Horton et al, 2013; Ouzounov et al, 2017), a number of research groups have successfully adopted and developed the technology (Chen et al, 2018; Escobet-Montalbán et al, 2018; Odríguez, 2018; Perillo et al, 2017; Rowlands et al, 2017; Takasaki et al, 2019b; Weisenburger et al, 2019; Yildirim et al, 2019), propelled by the commercially available lasers and microscopes. To facilitate 3PM applications, here we provide a quantitative characterization of three-photon deep-brain calcium imaging, with a side-by-side comparison to 2PM with 920 nm excitation.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of 1300-nm Three-photon Calcium Imaging in the Mouse Brain

Wang

Ouzounov

et al. 2019

Preprint

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1300-nm three-photon calcium imaging has emerged as a useful technique to allow calcium imaging in deep brain regions. Application to large-scale neural activity imaging entails a careful balance between recording fidelity and tissue heating. We calculated and experimentally verified the excitation pulse energy to achieve the minimum photon count required for the detection of calcium transients in GCaMP6s-expressing neurons for 920-nm two-photon and 1320-nm three-photon excitation, respectively. Brain tissue heating by continuous three-photon imaging was simulated with Monte Carlo method and experimentally validated with immunohistochemistry. We observed increased immunoreactivity with 150 mW excitation power at 1.0- and 1.2-mm imaging depths. Based on the data, we explained how three-photon excitation achieves better calcium imaging fidelity than two-photon excitation in the deep brain and quantified the imaging depth where three-photon microscopy should be applied. Our analysis presents a translatable model for the optimization of three-photon calcium imaging based on experimentally tractable parameters.

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Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of 1300-nm Three-photon Calcium Imaging in the Mouse Brain

Wang

Ouzounov

et al. 2019

Preprint

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“…As for other technical developments, a combination of the techniques described above is currently explored to circumvent these limitations. For instance, combining Bessel beam with 3P absorption offers higher volume rate (Chen et al, 2018;Rodríguez et al, 2018).…”

Section: Mpm Approaches To Probe Deep Cortical and Sub-cortical Brainmentioning

confidence: 99%

Wide. Fast. Deep: Recent Advances in Multiphoton Microscopy ofIn VivoNeuronal Activity

2019

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Multiphoton microscopy (MPM) has emerged as one of the most powerful and widespread technologies to monitor the activity of neuronal networks in awake, behaving animals over long periods of time. MPM development spanned across decades and crucially depended on the concurrent improvement of calcium indicators that report neuronal activity as well as surgical protocols, head fixation approaches, and innovations in optics and microscopy technology. Here we review the last decade of MPM development and highlight how in vivo imaging has matured and diversified, making it now possible to concurrently monitor thousands of neurons across connected brain areas or, alternatively, small local networks with sampling rates in the kilohertz range. This review includes different laser scanning approaches, such as multibeam technologies as well as recent developments to image deeper into neuronal tissues using new, long-wavelength laser sources. As future development will critically depend on our ability to resolve and discriminate individual neuronal spikes, we will also describe a simple framework that allows performing quantitative comparisons between the reviewed MPM instruments. Finally, we provide our own opinion on how the most recent MPM developments can be leveraged at scale to enable the next generation of discoveries in brain function.

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“…pulsed lasers typically have pulse duration and repetition rate below 70 fs and 1.25 MHz, [17][18][19][20][21]23 respectively. The Ti:Sapphire laser source in our system is typically used to generate 2P excitation, therefore, it is not optimal for 3P microscopy.…”

Section: Three-photon Trafixmentioning

confidence: 99%

Wide-field multiphoton imaging with TRAFIX

Escobet-Montalbán

Wijesinghe

Chen

et al. 2019

Multiphoton Microscopy in the Biomedical Sciences XIX

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Optical approaches have broadened their impact in recent years with innovations in both wide-field and superresolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multiphoton images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while significantly reducing photobleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.

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Rapid volumetric imaging with Bessel-Beam three-photon microscopy

Cited by 71 publications

References 23 publications

Quantitative Analysis of 1300-nm Three-photon Calcium Imaging in the Mouse Brain

Quantitative Analysis of 1300-nm Three-photon Calcium Imaging in the Mouse Brain

Wide. Fast. Deep: Recent Advances in Multiphoton Microscopy ofIn VivoNeuronal Activity

Wide-field multiphoton imaging with TRAFIX

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