Rapid Volumetric Optoacoustic Tracking of Individual Microparticles <i>In Vivo</i> Enabled by a NIR-Absorbing Gold–Carbon Shell

Nozdriukhin, Daniil; Kalva, Sandeep Kumar; Li, Weiye; Yashchenok, Alexey M.; Gorin, Dmitry A.; Razansky, Daniel; Deán‐Ben, Xosé Luís

doi:10.1021/acsami.1c15509

Cited by 11 publications

(3 citation statements)

References 73 publications

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“…Au/carbon nanocomposite materials are well-known for their excellent photothermal performance, which can be reflected by their efficient absorption in the near infrared (NIR) region. [30][31][32][33] Therefore, the absorption of Au@C-CCM was assessed using UVvisible spectroscopy, which showed a broad NIR absorption band as well as a liner relationship between the absorbance at 808 nm and NP concentration (Fig. 1e and Fig.…”

Section: Preparation and Characterization Of Au@c-ccmmentioning

confidence: 99%

A biomimetic nanoplatform for customized photothermal therapy of HNSCC evaluated on patient-derived xenograft models

Chen

Huang

et al. 2023

Int J Oral Sci

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Cancer cell membrane (CCM) derived nanotechnology functionalizes nanoparticles (NPs) to recognize homologous cells, exhibiting translational potential in accurate tumor therapy. However, these nanoplatforms are majorly generated from fixed cell lines and are typically evaluated in cell line-derived subcutaneous-xenografts (CDX), ignoring the tumor heterogeneity and differentiation from inter- and intra- individuals and microenvironments between heterotopic- and orthotopic-tumors, limiting the therapeutic efficiency of such nanoplatforms. Herein, various biomimetic nanoplatforms (CCM-modified gold@Carbon, i.e., Au@C-CCM) were fabricated by coating CCMs of head and neck squamous cell carcinoma (HNSCC) cell lines and patient-derived cells on the surface of Au@C NP. The generated Au@C-CCMs were evaluated on corresponding CDX, tongue orthotopic xenograft (TOX), immune-competent primary and distant tumor models, and patient-derived xenograft (PDX) models. The Au@C-CCM generates a photothermal conversion efficiency up to 44.2% for primary HNSCC therapy and induced immunotherapy to inhibit metastasis via photothermal therapy-induced immunogenic cell death. The homologous CCM endowed the nanoplatforms with optimal targeting properties for the highest therapeutic efficiency, far above those with mismatched CCMs, resulting in distinct tumor ablation and tumor growth inhibition in all four models. This work reinforces the feasibility of biomimetic NPs combining modular designed CMs and functional cores for customized treatment of HNSCC, can be further extended to other malignant tumors therapy.

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Section: Preparation and Characterization Of Au@c-ccmmentioning

confidence: 99%

A biomimetic nanoplatform for customized photothermal therapy of HNSCC evaluated on patient-derived xenograft models

Chen

Huang

et al. 2023

Int J Oral Sci

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“…Imaging Experiments-Whole-Body Mouse Imaging: Whole-body OPLUS imaging of mice was performed with a scanning approach equivalent to that used in spiral volumetric optoacoustic tomography (SVOT). [97,98 ] Much like for the experiments described above, the OPLUS bundle was inserted into the central aperture of the spherical array transducer with ≈1 cm offset toward the center of the array. A 24-week-old female athymic nude Foxn1nu mouse was anaesthetized with isoflurane in an O 2 -air mixture (100/400 mL mi −1 n, 5% for induction, 2% for maintenance) and placed on a custom-made holder with fore and hind paws fixed.…”

Section: Methodsmentioning

confidence: 99%

Multi‐Scale Volumetric Dynamic Optoacoustic and Laser Ultrasound (OPLUS) Imaging Enabled by Semi‐Transparent Optical Guidance

Nozdriukhin,

Kalva,

Özsoy

et al. 2023

Advanced Science

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Major biological discoveries are made by interrogating living organisms with light. However, the limited penetration of un‐scattered photons within biological tissues limits the depth range covered by optical methods. Deep‐tissue imaging is achieved by combining light and ultrasound. Optoacoustic imaging exploits the optical generation of ultrasound to render high‐resolution images at depths unattainable with optical microscopy. Recently, laser ultrasound has been suggested as a means of generating broadband acoustic waves for high‐resolution pulse‐echo ultrasound imaging. Herein, an approach is proposed to simultaneously interrogate biological tissues with light and ultrasound based on layer‐by‐layer coating of silica optical fibers with a controlled degree of transparency. The time separation between optoacoustic and ultrasound signals collected with a custom‐made spherical array transducer is exploited for simultaneous 3D optoacoustic and laser ultrasound (OPLUS) imaging with a single laser pulse. OPLUS is shown to enable large‐scale anatomical characterization of tissues along with functional multi‐spectral imaging of chromophores and assessment of cardiac dynamics at ultrafast rates only limited by the pulse repetition frequency of the laser. The suggested approach provides a flexible and scalable means for developing a new generation of systems synergistically combining the powerful capabilities of optoacoustics and ultrasound imaging in biology and medicine.

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“…More recently, larger microparticles have been suggested for guided drug delivery, and to enhance the resolution and quality of OA images. [22][23][24][25] Overall, contrast agents capable of being detected at a singleparticle level and being safe for human use can greatly impact biomedical research, foster new application fields, such as micro-robotics, and facilitate OA clinical translation. Yet, the development of such agents faces several challenges.…”

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confidence: 99%

Biobased Agents for Single‐Particle Detection with Optoacoustics

Chen

Nozdriukhin

Michel-Souzy

et al. 2023

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Optoacoustic (OA, photoacoustic) imaging capitalizes on the low scattering of ultrasound within biological tissues to provide high-resolution optical contrast at depths not reachable with optical microscopy. [1][2][3] OA has been widely used to visualize vascular networks, quantify oxygen saturation levels, and characterize healthy and diseased tissues based exclusively on the endogenous contrast provided by hemoglobin, melanin, lipids, and other chromophores. [4][5][6] However, the unique advantages of state-of-the-art OA systems stemming from the multi-spectral (multi-wavelength) and dynamic imaging capabilities can only be fully exploited with extrinsically-administered contrast agents. Contrast-enhanced OA imaging can improve the visibility of blood vessels, organs, and tissues, particularly in deep regions where light is significantly attenuated. [7,8] Optoacoustic (OA, photoacoustic) imaging synergistically combines rich optical contrast with the resolution of ultrasound within light-scattering biological tissues. Contrast agents have become essential to boost deep-tissue OA sensitivity and fully exploit the capabilities of state-of-the-art OA imaging systems, thus facilitating the clinical translation of this modality. Inorganic particles with sizes of several microns can also be individually localized and tracked, thus enabling new applications in drug delivery, microrobotics, or super-resolution imaging. However, significant concerns have been raised regarding the low bio-degradability and potential toxic effects of inorganic particles. Bio-based, biodegradable nano-and microcapsules consisting of an aqueous core with clinically-approved indocyanine green (ICG) and a crosslinked casein shell obtained in an inverse emulsion approach are introduced. The feasibility to provide contrast-enhanced in vivo OA imaging with nanocapsules as well as localizing and tracking individual larger microcapsules of 4-5 µm is demonstrated. All components of the developed capsules are safe for human use and the inverse emulsion approach is known to be compatible with a variety of shell materials and payloads. Hence, the enhanced OA imaging performance can be exploited in multiple biomedical studies and can open a route to clinical approval of agents detectable at a single-particle level.

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Rapid Volumetric Optoacoustic Tracking of Individual Microparticles In Vivo Enabled by a NIR-Absorbing Gold–Carbon Shell

Cited by 11 publications

References 73 publications

A biomimetic nanoplatform for customized photothermal therapy of HNSCC evaluated on patient-derived xenograft models

A biomimetic nanoplatform for customized photothermal therapy of HNSCC evaluated on patient-derived xenograft models

Multi‐Scale Volumetric Dynamic Optoacoustic and Laser Ultrasound (OPLUS) Imaging Enabled by Semi‐Transparent Optical Guidance

Biobased Agents for Single‐Particle Detection with Optoacoustics

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