“…Recently, several new high-throughput sequencing approaches such as 454 pyrosequencing, Illumina (Illumina, San Diego, CA, USA) and SOLiD (Applied Biosystems, Foster City, CA, USA) have been developed (Margulies et al, 2005;Hamady et al, 2008;Smith et al, 2008;Schulte et al, 2010). At present, the 454 pyrosequencing technology has been widely used to reveal microbial diversity and ecology in different natural environments, such as the deep sea (Sogin et al, 2006;Huber et al, 2007), extreme hydrogeological conditions (Edwards et al, 2006) and soil (Leininger et al, 2006;Roesch et al, 2007).…”