Rapidity of RNA synthesis in human cells

Fauris, C.; Danglot, C.; Vilagines, R.

doi:10.1016/0043-1354(85)90112-5

Cited by 28 publications

(8 citation statements)

References 20 publications

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“…These perturbations can be detected in the cell; rapidity of RNA synthesis of viable Hela S3 or Balb/c 3T3 cells is a cellular parameter which has been shown to be very sensitive (Shopsis and Sathe, 1984;Fauris et al, 1985). The 5 efficiency of this cytotoxic assay which is based on the quantitative inhibition of the RNA synthesis rate of the cells exposed to toxic compounds has been demonstrated by comparison of data with those obtained with the more classical physico-chemical determinations (Fauris et al, 1985;Fauris et Vilagines, 1988). This test is already being applied to the detection of pollutants in water using Hela S3 cell line (AFNOR, Association Française de Normalisation, 1996).…”

Section: Introductionmentioning

confidence: 85%

“…The original assay was first described by Fauris et al, (1985), adapted to HepG2 cells by Valentin et al, (2001) and finally automated by Valentin-Séverin et al, (2002). The latest assay was used here.…”

Section: Cytotoxicity Assaysmentioning

confidence: 99%

“…Thus, agents that stimulate or modify cell growth may manifest their actions by perturbations in nutrients uptake rates (Koren, 1980). These perturbations can be detected in the cell; rapidity of RNA synthesis of viable Hela S3 or Balb/c 3T3 cells is a cellular parameter which has been shown to be very sensitive (Shopsis and Sathe, 1984;Fauris et al, 1985). The 5 efficiency of this cytotoxic assay which is based on the quantitative inhibition of the RNA synthesis rate of the cells exposed to toxic compounds has been demonstrated by comparison of data with those obtained with the more classical physico-chemical determinations (Fauris et al, 1985;Fauris et Vilagines, 1988).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the sensitivity of three sublethal cytotoxicity assays in human HepG2 cell line using water contaminants

Jondeau¹,

Dahbi²,

Marie-Hélène³

et al. 2006

Toxicology

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tion of the sensitivity of three sublethal cytotoxicity assays in human HepG2 cell line using water contaminants. Toxicology, Elsevier, 2006. AbstractThe in vitro toxicological index IC50 (the millimolar concentration of compound which inhibits response assay by 50% compared to the solvent control) of 11 water contaminants (acrylamide, atrazine, B[a]P, BPA, 2,4-DAT, 17-αEE, H 2 O 2 , 4-OP, sodium bromate, sodium chlorate, sodium nitrate) was evaluated on the human hepatoma (HepG2) cells using three short-term bioassays related to their morbidity status [radiometric RNA synthesis assay (RNA), luminometric ATP assay (ATP), fluorometric Alamar blue assay (AB)]. Among all substances, we were not able to determine atrazine IC50 value whatever the test used. Furthermore, B[a]P was not cytotoxic in the ATP and AB assays. Statistical analysis revealed a correlation between the IC50 values obtained in the three assays. Except with 4-OP, RNA assay was always inhibited at lower concentrations than those required in the other assays, suggesting that this assay is a very sensitive indicator of the presence of toxic compounds. ATP and AB assays responded to a similar pattern. Due to its higher sensitivity and its reliability, RNA synthesis assay using HepG2 cell line provides the most suitable tool for the screening of water contaminants.3

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Section: Introductionmentioning

confidence: 85%

Section: Cytotoxicity Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the sensitivity of three sublethal cytotoxicity assays in human HepG2 cell line using water contaminants

Jondeau¹,

Dahbi²,

Marie-Hélène³

et al. 2006

Toxicology

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“…Migration of vinyl chloride from polyvinyl chloride bottles into drinking water increased in direct proportion to the storage time at a rate of 1 ng/dm 3 per day [133]. The total dosage of consumption of this monomer with drinking water may reach, in some cases, up to 100 ng per person per day.…”

Section: Effect Of Container Materials Coming Into Contact With Watementioning

confidence: 99%

Biotest as an Evaluation Method for the Quality of Drinking Water

Гончарук

2014

Drinking Water

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The comprehensive critical review of works on drinking water biotests, which is widely applied worldwide as the method to analyse the drinking water quality and risk evaluation for human health. The need to apply complex approach for biotests has been justified. Analysis result of 53 marks of bottled water by biotest methods has been introduced for the first time ever. The use of chemical methods for the assessment of natural water quality does not always yield necessary results, due to the lack of information on the biological components of the chemicals analyzed. Biotesting makes it possible to determine water toxicity, and it does not contain any harmful substances according to the chemical analysis. However, according to the biological criteria, the location of the toxicant agent does not indicate water toxicity. Moreover, the chemical examination does not take into consideration the interaction of contaminants and their transformation both in nature and in organisms. Biotesting methods assess objectively and comprehensively the influence of substances on an organism and its vital processes. In many cases biomonitoring is technically simpler and considerably cheaper than other alternatives. It does not require special instrumentation; it is limited in time; and it is more precise and sensitive in comparison with the chemical analysis. On the whole, biotesting will lead both to a reduction in the number of necessary procedures and to the significant simplification of the examination process. KeywordsMethods of biological assessment to determine the quality of aqueous environments may be divided into the methods of bioindication and biotesting. Biotesting V. V. Goncharuk, Drinking Water,

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“…The original assay was first described by Fauris, Danglot, and Vilagines (1985) and adapted to HepG2 cells by Valentin-Séverin, Laignelet, Lhuguenot, and Chagnon (2002). Briefly, tritiated uridine was added to the culture medium (10 lL, 0.3 lCi/well) and its amount of incorporation into the cellular RNA was measured (after 6, 12, 18, 24 and 30 min uptakes).…”

Section: Cytotoxicity: Rna Synthesis Inhibition Assaymentioning

confidence: 99%

The consequences of physical post-treatments (microwave and electron-beam) on food/packaging interactions: A physicochemical and toxicological approach

Riquet

Breysse²,

Dahbi

et al. 2016

Food Chemistry

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a b s t r a c tThe safety of microwave and electron-beam treatments has been demonstrated, in regards to the formation of reaction products that could endanger human health. An integrated approach was used combining the potential toxicity of all the substances likely to migrate to their chemical characterizations. This approach was applied to polypropylene (PP) films prepared with a selection of additives. Components were identified by liquid and gas chromatography using a mass selective detector system. Their potential toxicity was assessed using three in vitro short-term bioassays and their migrations were carried out using a standards-based approach. After the electron-beam treatment some additives decomposed and there was a significant increase in the polyolefin oligomeric saturated hydrocarbons concentration. PP prepared with Irgafos 168 led to a significantly strong cytotoxic effect and PP prepared with Irganox 1076 induced a dose-dependant estrogenic effect in vitro. Migration values were low and below the detection limit of the analytical method applied.

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Rapidity of RNA synthesis in human cells

Cited by 28 publications

References 20 publications

Evaluation of the sensitivity of three sublethal cytotoxicity assays in human HepG2 cell line using water contaminants

Evaluation of the sensitivity of three sublethal cytotoxicity assays in human HepG2 cell line using water contaminants

Biotest as an Evaluation Method for the Quality of Drinking Water

The consequences of physical post-treatments (microwave and electron-beam) on food/packaging interactions: A physicochemical and toxicological approach

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