Rapidly assessing changes in bone mineral balance using natural stable calcium isotopes

Morgan, JL; Skulan, Joseph L.; Gordon, Gwyneth W.; Romaniello, Stephen J.; Smith, Scott M.; Anbar, Ariel D.

doi:10.1073/pnas.1119587109

Cited by 121 publications

(113 citation statements)

References 36 publications

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“…*Described in ref. 59. including horses, seals, mice, deer, sheep, and Göttingen minipigs (30,(42)(43)(44)(45) and is assumed to be comparable in humans (46,47). The average δ 44/42 Ca value of wisdom tooth enamel that we measured in nine adult individuals (−1.58 ± 0.26 ‰, 2 SD, n = 9) is thus in good agreement with a hypothetical average Western diet composition of −1.00‰.…”

Section: Resultssupporting

confidence: 65%

“…First, compared with the estimated −1.00‰ mean value of diet, the mother's blood has lower δ 44/42 Ca values: a compilation of available data in mammals yields a Ca isotopic shift value from diet to blood, denoted Δ blood-diet and given by the difference between δ 44/42 Ca blood and δ 44/42 Ca diet , of −0.30 ± 0.13 ‰ (1 SD, Table S3). Second, increased bone turnover and possible transient bone loss in pregnant women (49,50) could also involve a decrease in blood δ 44/42 Ca values (46,47). Third, the transfer of Ca from maternal to fetal blood involves an active transport of Ca through the placenta (51), hypothetically responsible for preferential transport of light Ca isotopes (32,33).…”

Section: Resultsmentioning

confidence: 99%

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Assessing human weaning practices with calcium isotopes in tooth enamel

Tacail

Thivichon-Prince

Martin

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Weaning practices differ among great apes and likely diverged during the course of human evolution, but behavioral inference from the fossil record is hampered by a lack of unambiguous biomarkers. Here, we show that early-life dietary transitions are recorded in human deciduous tooth enamel as marked variations in Ca isotope ratios (δ 44/42 Ca). Using a sequential microsampling method along the enamel growth axis, we collected more than 150 enamel microsamples from 51 deciduous teeth of 12 different modern human individuals of known dietary histories, as well as nine enamel samples from permanent third molars. We measured and reconstructed the evolution of 44 Ca/ 42 Ca ratios in enamel from in utero development to first months of postnatal development. We show that the observed variations of δ 44/42 Ca record a transition from placental nutrition to an adult-like diet and that Ca isotopes reflect the duration of the breastfeeding period experienced by each infant. Typically, the δ 44/42 Ca values of individuals briefly or not breastfed show a systematic increase during the first 5-10 mo, whereas individuals with long breastfeeding histories display no measurable variation in δ 44/42 Ca of enamel formed during this time. The use of Ca isotope analysis in tooth enamel allows microsampling and offers an independent approach to tackle challenging questions related to past population dynamics and evolution of weaning practices in hominins.calcium isotopes | tooth enamel | dietary transitions | weaning | breast milk

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Section: Resultssupporting

confidence: 65%

Section: Resultsmentioning

confidence: 99%

Assessing human weaning practices with calcium isotopes in tooth enamel

Tacail

Thivichon-Prince

Martin

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…The isotopic fractionation of medium-mass endogen elements (i.e., Ca, Fe, Cu, and Zn) has recently been shown to have an outstanding potential for biomedical applications (1). Isotopic fractionation measurements have been identified as new biomarkers for the investigation of metabolic processes (2-6) and as powerful tools for the early detection of diseases (7)(8)(9)(10)(11)(12). Studies of Cu and Zn isotopic fractionation are very promising for the early diagnosis of different types of cancer (9,12) and neurodegenerative diseases (8,13), whereas Fe and Ca isotopic fractionation measurements have shown their ability to monitor Fe absorption efficiency (3) and bone mineral balance (7), respectively.…”

mentioning

confidence: 99%

Evidence of isotopic fractionation of natural uranium in cultured human cells

Paredes

Avazeri

Malard

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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The study of the isotopic fractionation of endogen elements and toxic heavy metals in living organisms for biomedical applications, and for metabolic and toxicological studies, is a cutting-edge research topic. This paper shows that human neuroblastoma cells incorporated small amounts of uranium (U) after exposure to 10 μM natural U, with preferential uptake of the 235 U isotope with regard to 238 U. Efforts were made to develop and then validate a procedure for highly accurate n( 238 U)/n( 235 U) determinations in microsamples of cells. We found that intracellular U is enriched in 235 U by 0.38 ± 0.13‰ (2σ, n = 7) relative to the exposure solutions. These in vitro experiments provide clues for the identification of biological processes responsible for uranium isotopic fractionation and link them to potential U incorporation pathways into neuronal cells. Suggested incorporation processes are a kinetically controlled process, such as facilitated transmembrane diffusion, and the uptake through a highaffinity uranium transport protein involving the modification of the uranyl (UO 2 2+ ) coordination sphere. These findings open perspectives on the use of isotopic fractionation of metals in cellular models, offering a probe to track uptake/transport pathways and to help decipher associated cellular metabolic processes.isotopic fractionation | uranium | neuronal cells | analytical procedure development | microsamples

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“…[10][11][12][13]. Similarly, it has great value in the early diagnosis and evaluation of therapies [14]. The isotopic abundance of a molecule can be altered in different ways, such as chemical reactions, etc.…”

Section: 4-dichlorobenzene (Para-dichlorobenzene or P-dcbmentioning

confidence: 99%

Determination of Isotopic Abundance Ratio of Biofield Energy Treated 1,4-Dichlorobenzene Using Gas Chromatography-Mass Spectrometry (GC-MS)

Trivedi¹

2016

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Abstract:The objective of the current study was to evaluate the effect of biofield energy treatment on the isotopic abundance ratios of P M+1 /P M , P M+2 /P M , P M+3 /P M and P M+4 /P M in p-DCB using gas chromatography-mass spectrometry (GC-MS). The p-DCB was divided into two parts -one part was control sample, and another part was considered as the treated sample which was subjected to biofield energy treatment (The Trivedi Effect ® ). T1, T2, T3, and T4 were referred the biofield treated p-DCB having analyzed at different time intervals. The GC-MS analysis of both the control and biofield treated p-DCB indicated the presence of the parent molecular ion peak at m/z 146 along with four major fragmentation peaks at m/z 111, 75, 55 and 50. The relative peak intensities of the fragmented ions in the biofield treated p-DCB were notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis using GC-MS revealed that the isotopic abundance ratio of P M+1 /P M at T1, T2, T3, and T4 (biofield energy treated p-DCB) was significantly increased by 10.87, 83.90, 225.16, and 241.15%, respectively as compared to the control sample. Consequently, the percentage change in the isotopic abundance ratio of P M+2 /P M at T1, T2, and T3 (biofield energy treated p-DCB) was enhanced by 4.55, 9.49, and 1.80%, respectively as compared to the control sample. Beside these, another two isotopic molecular ion peaks at m/z 149 and 150 were found in the GS-MS spectra due to arise from the contributions of various combinations of 2 H, 13 C, and 37Cl. The isotopic abundance ratios of P M+3 /P M in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 15.14, 82.57, 192.43, and 218.31%, respectively as compared to the control sample. Similarly, the P M+4 /P M in biofield energy treated sample at T1, T2, T3, and T4 was significantly increased by 13.80, 86.66, 186.13, and 204.29%, respectively as compared to the control sample. Overall, the isotopic abundance ratios of P M+1 /P M ( Cl/ 35 Cl) were significantly enhanced in the biofield energy treated p-DCB. The biofield treated p-DCB has shown improved isotopic abundance ratios that might have altered the physicochemical properties, thermal properties and rate of reaction. Biofield treated p-DCB might be useful in pharmaceutical and chemical industries as intermediates during the manufacturing of pharmaceuticals and chemicals by monitoring the rate of chemical reaction.

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Rapidly assessing changes in bone mineral balance using natural stable calcium isotopes

Cited by 121 publications

References 36 publications

Assessing human weaning practices with calcium isotopes in tooth enamel

Assessing human weaning practices with calcium isotopes in tooth enamel

Evidence of isotopic fractionation of natural uranium in cultured human cells

Determination of Isotopic Abundance Ratio of Biofield Energy Treated 1,4-Dichlorobenzene Using Gas Chromatography-Mass Spectrometry (GC-MS)

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