Rapidly fatal infection with Bacillus cereus/thuringiensis: genome assembly of the responsible pathogen and consideration of possibly contributing toxins

Butcher, Monica; Puiu, Daniela; Romagnoli, Mark; Carroll, Karen C.; Salzberg, Steven L.; Nauen, David W.

doi:10.1016/j.diagmicrobio.2021.115534

Cited by 4 publications

(3 citation statements)

References 46 publications

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“…According to the CAZy data B. thuringiensis Q1 contained one polysaccharide lyase and 155 glycoside hydrolases. Some bacterial extracellular enzymes were identified as involved in algicidal activity, such as glycoside hydrolases and polysaccharide lyases, which can be induced by the presence of microalgae [ [10] , [30] ], while disrupting the cell wall.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Bacillus thuringiensis Q1 compound with algicidal activity

Qiao

Zhang

2023

Heliyon

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Section: Discussionmentioning

confidence: 99%

Identification of a Bacillus thuringiensis Q1 compound with algicidal activity

Qiao

Zhang

2023

Heliyon

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“…The diseases are generally mild and self-limiting, making it difficult for health authorities to determine the actual incidence [ 3 ]. The proportion of B. cereus infections is underestimated, and severe and fatal outbreaks have also been reported [ 4 ]. Despite the optimized combination of sterilization technologies such as heat, irradiation, and chemical reagents, B. cereus can still survive and pose a formidable challenge for food industries worldwide [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis of Stress Resistance of Bacillus cereus SCL10 Strain Based on Whole-Genome Sequencing

Mao,

Yang,

Lin

et al. 2024

Microorganisms

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A Gram-positive, rod-shaped, aerobic, motile, and spore-forming bacterium, designated SCL10, was isolated from Acaudina molpadioides exposure to Co-60 radiation. In this study, whole-genome sequencing was performed to identify the strain as Bacillus cereus and functional characterization, with a focus on stress resistance. The genome of the B. cereus SCL10 strain was sequenced and assembled, revealing a size of 4,979,182 bp and 5167 coding genes. The genes involved in biological functions were annotated by using the GO, COG, KEGG, NR, and Swiss-Prot databases. The results showed that genes related to alkyl hydroperoxide reductase (ahpC, ahpF), DNA-binding proteins from starved cells (dps), spore and biofilm formation (spoVG, spo0A, gerP), cold shock-like protein (cspC, cspE), ATP-dependent chaperone (clpB), and photolyase, small, acid-soluble spore protein (SASP) and DNA repair protein (recA, radD) could explain the stress resistance. These findings suggest that antioxidant activity, sporulation, biofilm formation, and DNA protection may be considered as the main resistance mechanisms under exposure to radiation in the B. cereus SCL10 strain.

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“…On the other hand, when B. cereus is detected in sterile samples of the human body, the spores are extensively distributed in a hospital environment; therefore, the situation is often regarded as contamination. However, an increasing number of studies suggested that B. cereus causes serious septicemia (bacteremia) [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21]. In addition, the administration of peripheral parenteral nutrition containing glucose, amino acids, and electrolytes (PPN) [22][23][24][25] and the summer season [26][27][28] have been indicated as risk factors for B. cereus-related septicemia.…”

Section: Introductionmentioning

confidence: 99%

Peripheral Parenteral Nutrition Solutions and Bed Bath Towels as Risk Factors for Nosocomial Peripheral Venous Catheter-related Bloodstream Infection by Bacillus cereus

Hino¹,

Ozaki²,

Kitahara³

et al. 2023

Int. J. Med. Sci.

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In Japan, China, and Singapore, several studies have reported increased incidences of peripheral venous catheter-related bloodstream infection by Bacillus cereus during the summer. Therefore, we hypothesized that bed bathing with a B. cereus -contaminated “clean” towels increases B. cereus contact with the catheter and increases the odds of contaminating the peripheral parenteral nutrition (PPN). We found that 1) professionally laundered “clean” towels used in hospitals have B. cereus (3.3×10 4 colony forming units (CFUs) / 25cm 2 ), 2) B. cereus is transferable onto the forearms of volunteers by wiping with the towels (n=9), and 3) B. cereus remain detectable (80∼660 CFUs /50cm 2 ) on the forearms of volunteers even with subsequent efforts of disinfection using alcohol wipes. We further confirmed that B. cereus grow robustly (10 2 CFUs /mL to more than 10 6 CFUs /mL) within 24hours at 30°C in PPN. Altogether we find that bed bathing with a towel contaminated with B. cereus leads to spore attachments to the skin, and that B. cereus can proliferate at an accelerated rate at 30°C compared to 20°C in PPN. We therefore highly recommend ensuring the use of sterile bed bath towels prior to PPN administration with catheter in patients requiring bed bathing.

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Rapidly fatal infection with Bacillus cereus/thuringiensis: genome assembly of the responsible pathogen and consideration of possibly contributing toxins

Cited by 4 publications

References 46 publications

Identification of a Bacillus thuringiensis Q1 compound with algicidal activity

Identification of a Bacillus thuringiensis Q1 compound with algicidal activity

Functional Analysis of Stress Resistance of Bacillus cereus SCL10 Strain Based on Whole-Genome Sequencing

Peripheral Parenteral Nutrition Solutions and Bed Bath Towels as Risk Factors for Nosocomial Peripheral Venous Catheter-related Bloodstream Infection by Bacillus cereus

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