Rapidly labeled intermediates in DNA replication in higher plants

Discontinuous DNA Synthesis in Mammalian Cells

“…For the Okazaki fragments of other organisms there have been a wide range of si ze s reported, ranging from 1000-2 000 nucl eoti de s (Kidwell & Mueller, 1969;Mueller, 1969;Painter & Schaefer, 1969;8chandl & Taylor, 19698chandl & Taylor, , 1971Taylor et a1., 1970Taylor et a1., , 1973aBaker, 1971;Bellett & Younghusband, 1972;Cheevers et a1., 1972;Goldstein & Rutman, 1973;Friedman, 1974;Sakamaki et al, 1975;Vlak et a1., 1975), to 300 nucleotides (Nuzzo et a1., 1970) to 50-200 nucleotides (Hyodo et a1. , 1971;Fareed & Salzman & Thoren, 1973;Francke & Hunter, 1974a, b;Kriegstein & Hogness, 1974;Pigiet et a1., 1974;Qasba, 1974b;Friedman et a1., 1975;Gautschi & Clarkson, 1975;Mendelsohn 8t a1., 1975;Tseng & Goulian, 1975a) to 10-20 nucleotides (Schandl & Taylor, 1969Taylor et a1., 1970;Schandl, 1972).…”

“…Such evidence has been obtained both in in vivo studies (Painter & Schaefer, 1969;Schandl & Taylor, 1969;Baker, 1971;Cheevers et aL, 1972;Fareed & Salzman, 1972;Laipis & Levine, 1973;Magnusson, 1973a;Berger & Huang, 1974;Qasba, 1974b;Gautschi & Clarkson, 1975;Mendelsohn et al, 1975;Tseng & Goulian, 1975a;Vlak et al, 1975) and in more rigorous in vitro experiments (Kidwell & Mueller, 1969;Mueller, 1969;Magnusson, 1973;Magnusson et aL, 1973;Francke & Hunter, 1974a, b;Qasba, 1974a;Hunter & Francke, 1975;Otto & Reichard, 1975;Tseng & Goulian, 1975a;Fraser, unpublished sedimenting at a position between the Okazaki and bulk peaks (Painter, 1968;Taylor & Miner, 1968;Habener et al, 1969b;Kidwell & Mueller, 1969;Mueller, 1969;Schandl & Taylor, 1969;Berger & Irvin, 1970;Hyodo et al, 1970Hyodo et al, , 1971Chiu & Rauth, 1972, Gautschi 8t al., 1973Goldstein & Rutman, 1973;Berger & Huang, 1974;Kohn et al, 1974;Friedman et al, 1975;Rajalakshmi & Sarma, 1975;Sak...…”

Differences in timing of replication and maturation of nascent DNA fragments between satellite and main band DNA during the S phase of the first cell cycle in cultured carrot root explants.

“…Single-stranded DNA fragments of 5-6S and 8-9S were detected after a pulse for 5-6 min in Vicia faba embryonic axes. After prolonged labeling for 20 min, a large portion of the labeled DNA was found in the 7-17S region of an alkaline sucrose gradient, with a main peak of 10-11S (Sakamaki et al, 1975).…”

“…When embryonic axes, detached from germinating Vicia faba seeds, were pulse-labeled with 3H-thymidine, short and single-stranded DNA molecules of 5-6S and 8-9S (or lOS) appeared (Sakamaki et al, 1975(Sakamaki et al, , 1976Yakura and Tanifuji, 1980). Such short nascent DNA fragments have been observed also in radicles of germinating cotton seeds (Clay et al, 1976), root tips of pea seedlings (Schvartzman et al, 1981), and cultured soybean cells (Cress et al, 1978).…”

Mechanism of nuclear DNA replication in radicles of germinating cotton

“…4A). Recent evidence also indicates that radioactivity appears first in small-molecular-weight molecules (10S) of DNA after pulse-labeling in Vicia faba embryos (27). With the progression of labeling time, the radioactivity appeared predominantly in a fraction of approximately 25 S and after 90 min, 99% of the label appeared in the larger-molecular-weight 25S peak (Fig.…”